System and device

Patent metadata

Field	Value
Publication number	US-12370542-B2
Application number	US-201816613018-A
Country	US
Kind code	B2
Filing date	May 11, 2018
Priority date	May 12, 2017
Publication date	Jul 29, 2025
Grant date	Jul 29, 2025

Abstract

A cassette for use in concentrating matter in a sample suspension, the cassette comprising a housing having a support and an enclosed sample-receiving channel, the enclosed sample-receiving channel having an upper portion and a base connected by at least two walls; in which the upper portion is configured to have a width less than a width of the base and a depth greater than 400 μm.

First claim

The invention claimed is:

1. A cassette ( 1 , 200 , 300 ) for use in concentrating particulate matter in a sample suspension, the cassette ( 1 , 200 , 300 ) comprising a housing ( 2 ) having a support ( 5 ), an inlet port ( 20 ) adapted to receive the sample suspension; and an enclosed sample-receiving channel ( 6 ) in fluid communication with the inlet port ( 20 ), the enclosed sample-receiving channel ( 6 ) having an upper portion ( 7 ) and a base ( 5 a ) connected by at least two walls ( 11 a , 11 b ); in which the upper portion ( 7 ) is configured to have a width less than a width of the base ( 5 a ) and a depth greater than 400 μm; in which the inlet port ( 20 ) is configured to act as a conduit to deliver the sample suspension to the enclosed sample-receiving channel ( 6 ); and in which the walls ( 11 a , 11 b ) of the enclosed sample-receiving channel ( 6 ) have a slope of between 40° and less than 90° with respect to the base ( 5 a ); characterised in that the enclosed sample-receiving channel ( 6 ) further comprises a single raised platform ( 50 ) extending upwards from the base ( 5 a ) and extending along the length of the enclosed sample-receiving channel ( 6 ); in which the raised platform ( 50 ) is separated from the upper portion ( 7 ) and both of the walls ( 11 a , 11 b ), and wherein the depth of the upper portion ( 7 ) of the enclosed sample-receiving channel ( 6 ) is measured from the top of the raised platform ( 50 ); wherein the height of the raised platform ( 50 ) is greater than or equal to the depth of the enclosed sample-receiving channel ( 6 ) measured from the top of the raised platform ( 50 ) and wherein the width of the raised platform ( 50 ) is greater than or equal to the width of the upper portion ( 7 ) of the enclosed sample-receiving channel ( 6 ); and wherein the combination of the enclosed sample-receiving channel ( 6 ) and the raised platform ( 50 ) extending upward from the base ( 5 a ) results in a sample concentrating effect on buoyant particulate matter in the sample suspension in the enclosed sample-receiving channel ( 6 ).

2. The cassette ( 1 , 200 , 300 ) according to claim 1 , in which the base ( 5 a ) is formed by the housing support ( 5 ).

3. The cassette ( 1 , 200 , 300 ) according to claim 1 , in which the housing ( 2 ) further comprises an air release port ( 14 ) in fluid communication with the enclosed sample-receiving channel ( 6 ).

4. The cassette ( 1 , 200 , 300 ) according to claim 3 , in which the air release port ( 14 ) is diametrically opposed to the inlet port ( 20 ) or the air release port ( 14 ) is opposite the inlet port ( 20 ).

5. The cassette ( 1 , 200 , 300 ) according to claim 1 or claim 4 , in which the inlet port ( 20 ) is a non-return valve.

6. The cassette ( 200 ) according to claim 1 , in which the housing ( 2 ) of the cassette ( 1 ) consists of a lower section ( 201 ) configured to accommodate the enclosed sample-receiving channel ( 6 ) and an upper section ( 202 ) configured to reversibly attach to and create a seal with the lower section ( 201 ).

7. The cassette ( 1 , 200 , 300 ) according to claim 1 , wherein the housing further comprises a hole ( 30 ) configured to receive an actuator ( 101 ) connected to an image capturing device ( 100 ).

8. The cassette ( 1 , 200 , 300 ) according claim 1 , in which the cassette ( 1 , 200 , 300 ) is configured to move in one plane.

9. The cassette ( 1 , 200 , 300 ) according to claim 8 , wherein the motion within the plane can be rectilinear-translation or rotation, or a combination thereof.

10. The cassette ( 1 , 200 , 300 ) according claim 1 , in which an internal surface of the walls ( 11 a , 11 b ) of the enclosed sample-receiving channel ( 6 ) is coated with a hydrophobic material or a hydrophilic material to reduce surface tension along the walls of the channel to prevent adhesion of the particulate matter.

11. The cassette ( 1 , 200 , 300 ) of claim 1 , wherein the sample suspension comprises a flotation solution of higher density than the particulate matter in the sample suspension.

12. The cassette ( 1 , 200 , 300 ) of claim 11 , wherein the flotation solution is selected from a saturated NaCl solution, a saturated sugar solution, a Sheather's sugar solution, a saturated zinc sulphate solution, a saturated sodium nitrate solution, or a saturated magnesium sulphate solution.

Assignees

• Univ College Dublin Nat Univ Ireland Dublin

Inventors

• De Waal Theo
• Ebrahimi Roohollah
• Krivoruchko Michael
• Lobaskin Vladimir
• Mcowan Trish
• O'Connor William
• Scholz Dimitri

Classifications

• G01N2015/019

  Biological contaminants; Fouling · CPC title

• G01N15/1433

  using image recognition · CPC title

• G01N2015/1488

  Methods for deciding · CPC title

• G01N2015/1486

  Counting the particles · CPC title

• G01N15/1456 Primary

  without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals · CPC title