Apparatus for localizing single fluorescent molecules comprised in a sample using single molecule localization microscopy
US-2024393250-A1 · Nov 28, 2024 · US
US12366530B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12366530-B2 |
| Application number | US-202118253016-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 12, 2021 |
| Priority date | Nov 18, 2020 |
| Publication date | Jul 22, 2025 |
| Grant date | Jul 22, 2025 |
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A method for determining UV coverage on an irradiation target in real time comprises the steps of irradiating an irradiation target ( 10 ) with light including UV light ( 16 ) immediately after applying a sunscreen ( 12 ); detecting an amount of fluorescent light ( 20 ) emitted from the irradiation target in response to the light irradiation and storing the amount of the fluorescent light as reference data; irradiating the irradiation target ( 10 ) with the light including UV light ( 16 ) after a predetermined passage of time, or after applying a predetermined stress on the sunscreen ( 12 ); detecting an amount of fluorescent light ( 20 ) emitted from the irradiation target in response to the light irradiation and storing the amount of the fluorescent light as measurement data; and determining UV coverage by dividing the amount of the fluorescent light of the reference data by the amount of the fluorescent light of the measurement data, wherein the irradiation target ( 10 ) includes at least one substance emitting fluorescent light in response to the light irradiation.
Opening claim text (preview).
The invention claimed is: 1. A method for determining UV coverage on an irradiation target in real time, comprising steps of: irradiating an irradiation target with light immediately after applying a sunscreen; detecting an amount of fluorescent light emitted from the irradiation target in response to the light irradiation and storing the amount of the fluorescent light as reference data; irradiating the irradiation target with light after a predetermined passage of time, or after applying a predetermined stress on the sunscreen; detecting an amount of fluorescent light emitted from the irradiation target in response to the light irradiation and storing the amount of the fluorescent light as measurement data; and determining a decrease of UV coverage by obtaining a value by dividing the amount of the fluorescent light of the reference data with the amount of the fluorescent light of the measurement data, and subtracting the value from 1, wherein the irradiation target includes at least one substance emitting fluorescent light in response to the light irradiation. 2. The method according to claim 1 , wherein the reference data and the measurement data are raw data output from a sensor or normalized data with root mean square of amounts of the fluorescent light detected over a predetermined wavelength range. 3. The method according to claim 1 , wherein the light irradiating the irradiation target is ultraviolet light in a wavelength range of UV-A or UV-B. 4. The method according to claim 1 , further comprising a step of providing the determined UV coverage to a user as a color indication. 5. The method according to claim 1 , further comprising a step of providing an alert of reapplying the sunscreen to a user when the UV coverage is less than a predetermined threshold value. 6. The method according to claim 1 , wherein the irradiation target is a dummy sample including at least one substance included in human skin and emitting fluorescent light in response to irradiation of ultraviolet light, or wherein the irradiation target is human skin. 7. A device for determining UV coverage in real time, comprising: a light source for irradiating an irradiation target with light, wherein a sunscreen is applied to the irradiation target; an optical sensor for detecting an amount of fluorescent light emitted from the irradiation target in response to the light irradiation; and a control unit for controlling the light source and the optical sensor, wherein the control unit is configured to store the amount of the fluorescent light emitted from the irradiation target in response to the light irradiation immediately after applying the sunscreen on the irradiation target as reference data, wherein the control unit is configured to store the amount of the fluorescent light emitted from the irradiation target in response to the light irradiation after a predetermined passage of time from the application of the sunscreen or after applying a predetermined stress on the sunscreen as measurement data, wherein the control unit is configured to determine a decrease of UV coverage by obtaining a value by dividing the amount of the fluorescent light of the reference data with the amount of the fluorescent light of the measurement data, and subtracting the value from 1, and wherein the irradiation target comprises at least one substance emitting fluorescent light in response to the light irradiation. 8. The device according to claim 7 , wherein the control unit is configured to use raw data output from the optical sensor as the reference data and the measurement data, or configured to normalize the reference data and the measurement data with root mean square of the amounts of the fluorescent light detected over a predetermined wavelength range. 9. The device according to claim 7 , wherein the light source is configured to emit ultraviolet light within a wavelength range of UV-A or UV-B. 10. The device according to claim 7 , further comprising a display unit configured to provide the determined UV coverage to a user as a color indication. 11. The device according to claim 7 , wherein the control unit is configured to provide an alert of reapplying the sunscreen to a user when the UV coverage is less than a predetermined threshold value. 12. The device according to claim 7 , wherein the irradiation target is a dummy sample comprising at least one substance included in human skin and emitting fluorescent light in response to irradiation of ultraviolet light, or wherein the irradiation target is human skin.
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