Multifunctional lactobacillus capable of relieving PFOA toxic effects and application thereof

Patent metadata

Field	Value
Publication number	US-12364722-B2
Application number	US-202117562081-A
Country	US
Kind code	B2
Filing date	Dec 27, 2021
Priority date	Jun 28, 2019
Publication date	Jul 22, 2025
Grant date	Jul 22, 2025

Abstract

The present disclosure discloses multifunctional lactobacilli capable of relieving a toxic effect of perfluorooctanoic acid (PFOA) and use thereof, and belongs to the technical field of microorganisms. Lactobacillus fermentum CCFM1051, Lactobacillus casei CCFM1052 and Lactobacillus buchneri CCFM1053 of the present disclosure have high adsorption effect on PFOA, can significantly relieve liver oxidative stress damage and serum biochemical indicators caused by PFOA, spleen atrophy caused by PFOA exposure, imbalance of intestinal microorganisms caused by PFOA exposure and metabolic disorder of intestinal flora caused by PFOA exposure, significantly increase the content of acetic acid and propionic acid in the intestinal tract, increase the fecal water content and decrease first black stool defecation time of mice with constipation, improve proliferation and MafA gene expression of INS-1 cells under high glucose, have the potential to relieve PFOA-related diabetes, reduce occurrence of liver diseases, metabolic diseases and potential carcinogenicity, and have broad use prospects.

First claim

What is claimed is:

1. A composition, comprising:
a cytoprotective agent selected from one or more of ethylene glycol, propylene glycol, polyvinylpyrrolidone (PVP), polyethylene glycol, and hydroxyethyl starch, and
one or more of the following lactobacilli:
(a) Lactobacillus fermentum CCFM1051, deposited in Guangdong Microbial Culture Collection Center on Apr. 29, 2019, and having the preservation number of GDMCC No: 60649;
(b) Lactobacillus casei CCFM1052, deposited in Guangdong Microbial Culture Collection Center on Apr. 29, 2019, and having the preservation number of GDMCC No: 60650; and
(c) Lactobacillus buchneri CCFM1053, deposited in Guangdong Microbial Culture Collection Center on Apr. 29, 2019, and having the preservation number of GDMCC No: 60651,
wherein the composition is freeze-dried.

2. The composition according to claim 1 , wherein the fermentation composition is fermented by Lactobacillus fermentum CCFM1051, Lactobacillus casei CCFM1052, or Lactobacillus buchneri CCFM1053.

3. The composition according to claim 1 , wherein the composition comprises the lactobacilli in an amount greater than or equal to 1×10 8 CFU/mL or in an amount greater than or equal to 1×10 8 CFU/g.

4. The composition according to claim 1 , wherein the composition further comprises one or more pharmaceutically acceptable carriers.

5. The composition according to claim 4 , wherein the one or more pharmaceutically acceptable carriers is selected from one or more of: lactose, dextrose, sorbitol, mannitol, starch, arabic gum, calcium phosphate, alginate, tragacanth gum, gelatin, calcium silicate, microcrystalline cellulose, cellulose, water, methylcellulose, saline, and syrup.

Assignees

• Univ Jiangnan

Inventors

• Wang Gang
• Chen Wei
• Zhao Jianxin
• Zhang Hao
• Liang Xi
• Kong Qingmin
• Zhai Qixiao
• Lu Wenwei
• Cui Shumao
• Yang Bo
• Mao Bingyong

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12R2001/225

  Lactobacillus · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• C12N1/205

  Bacterial isolates · CPC title

• C12R2001/245

  Lactobacillus casei · CPC title