Nucleic acid purification method

Patent metadata

Field	Value
Publication number	US-12359189-B2
Application number	US-202017778406-A
Country	US
Kind code	B2
Filing date	Sep 9, 2020
Priority date	Nov 20, 2019
Publication date	Jul 15, 2025
Grant date	Jul 15, 2025

Abstract

The present application relates to a nucleic acid purification method, specifically to a nucleic acid purification method which includes a first step of crystallizing the nucleic acid using a solution containing a hydrophilic organic solvent; and a second step of drying the crystallized nucleic acid with high-humidity hot air.

First claim

The invention claimed is:

1. A nucleic acid purification method, the method comprising:
a first step of crystallizing the nucleic acid using a solution containing a hydrophilic organic solvent; and
a second step of drying the crystallized nucleic acid with air having a temperature of 30° C. or more and 90° C. or less and a relative humidity of 40% or more and 90% or less.

2. The nucleic acid purification method of claim 1 ,
wherein the nucleic acid includes any one or more selected from the group consisting of guanosine 5′-monophosphate disodium salt and inosine 5′-monophosphate disodium salt.

3. The nucleic acid purification method of claim 1 ,
wherein the nucleic acid includes guanosine 5′-monophosphate disodium salt heptahydrate or inosine 5′-monophosphate disodium salt 7.5 hydrate.

4. The nucleic acid purification method of claim 1 ,
wherein the hydrophilic organic solvent is any one or more selected from the group consisting of methanol and ethanol.

5. The nucleic acid purification method of claim 1 ,
wherein the air has a temperature of 30° C. or more and less than 60° C. and a relative humidity of 40% or more and 90% or less.

6. The nucleic acid purification method of claim 1 ,
wherein the air has a temperature of 70° C. or more and 90° C. or less and a relative humidity of 70% or more and 90% or less.

Assignees

• Cj Cheiljedang Corp

Inventors

• Lim Hwayeon
• Kim Min Jong
• Oh Changyub
• Kim Il Chul
• Kim Gyeonghwan
• Kim Yu Shin

Classifications

• C30B7/04

  using aqueous solvents · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12N15/1003 Primary

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• C07H19/167

  with ribosyl as the saccharide radical · CPC title

• C07H19/16

  Purine radicals · CPC title