Detection device and detection method

Patent metadata

Field	Value
Publication number	US-12352694-B2
Application number	US-202117523583-A
Country	US
Kind code	B2
Filing date	Nov 10, 2021
Priority date	Jul 3, 2019
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

A detection device is for detecting a detection target substance using a metal material modified with a first substance having a property of specifically binding to the detection target substance and a fluorescent material modified with a second substance having a property of specifically binding to the detection target substance. The detection device includes: a light source configured to emit light for exciting the fluorescent material; a photodetector configured to detect fluorescence emitted by the fluorescent material over time for a specific period from when emission of the light by the light source is stopped; and a processor configured to detect the detection target substance in a complex formed of the metal material, the detection target substance, and the fluorescent material binding to each other.

First claim

What is claimed is:

1. A detection device for detecting a detection target substance using a metal material modified with a first substance having a property of specifically binding to the detection target substance and a fluorescent material modified with a second substance having a property of specifically binding to the detection target substance, the detection device comprising:
a light source configured to emit light for exciting the fluorescent material;
a photodetector configured to detect fluorescence emitted by the fluorescent material over time for a specific period from when emission of the light by the light source is stopped; and
a processor configured to:
(i) determine whether an intensity of the fluorescence detected by the photodetector is greater than or equal to a threshold intensity, wherein when it is determined that the intensity of the fluorescence is greater than or equal to the threshold intensity, the effect of fluorescence caused by nonspecific adsorption of the fluorescent material is configured to be reduced; and
(ii) detect the detection target substance found within a complex formed of the metal material, the detection target substance, and the fluorescent material modified with the second substance.

2. The detection device according to claim 1 , wherein:
the processor is configured to detect the detection target substance found within the complex formed of the metal material, the detection target substance, and the fluorescent material modified with the second substance based on information which indicates whether a time, from when the light source stops emitting the light to when a ratio of the intensity of the fluorescence emitted by the fluorescent material to a maximum intensity of the fluorescence is equal to a specific ratio, is less than or equal to a threshold time.

3. The detection device according to claim 1 , wherein;
the processor is configured to detect the detection target substance found within the complex formed of the metal material, the detection target substance, and the fluorescent material modified with the second substance based on information which indicates whether a ratio of an amount of attenuation of the intensity of the fluorescence to a maximum intensity of the fluorescence during a period from when the light source stops emitting the light to when a specific time elapses is greater than or equal to a threshold.

4. The detection device according to claim 1 , wherein
the metal material is in a form of particles.

5. The detection device according to claim 1 , wherein
the metal material is a metal microstructure on a substrate.

6. The detection device according to claim 1 , wherein
the metal material contains, as a main component, metal on which localized surface plasmon resonance corresponding to a frequency of the light emitted by the light source or a fluorescence frequency of the fluorescent material occurs.

7. A detection device comprising:
a light source configured to emit light to a sample liquid containing a fluorescent material modified with a second substance;
a photodetector configured to detect first and second intensities of fluorescence emitted by the fluorescent material excited by the light, for a specific period after emission of the light is stopped; and
a processor configured to:
(i) determine whether the first intensity is greater than or equal to a threshold intensity, wherein when it is determined that the first intensity is greater than or equal to the threshold intensity, the effect of fluorescence caused by nonspecific adsorption of the fluorescent material is configured to be reduced; and
(ii) determine whether the sample liquid contains a detection target substance based on the second intensity,
wherein the detection target substance directly binds to the second substance, and the detection target substance binds to a metal material with a first substance therebetween.

8. The detection device according to claim 7 , wherein
the processor is configured to determine whether the first intensity is greater than or equal to the threshold intensity, wherein when it is determined that the first intensity is greater than or equal to the threshold intensity, the effect of fluorescence emitted by the fluorescent material directly binding to the metal material is configured to be reduced.

9. The detection device according to claim 1 , wherein
the processor is configured to determine whether the intensity of the fluorescence detected by the photodetector is greater than or equal to the threshold intensity, wherein when it is determined that the intensity of the fluorescence is greater than or equal to the threshold intensity, the effect of fluorescence emitted by the fluorescent material directly binding to the metal material is configured to be reduced.

10. The detection device according to claim 4 , wherein:
the metal material is neither directly nor indirectly fixed on a sensor cell which houses the detection target substance and the fluorescent material; and
the metal material is neither directly nor indirectly fixed on a substrate.

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Takeda Eiji

Classifications

• G01N33/56983

  Viruses · CPC title

• G01N21/6408 Primary

  with measurement of decay time, time resolved fluorescence · CPC title

• G01N33/582

  with fluorescent label · CPC title

• G01N2021/6439

  with indicators, stains, dyes, tags, labels, marks · CPC title

• G01N21/648 Primary

  using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title