Dna modifying enzymes and active fragments and variants thereof and methods of use
US-2024301385-A1 · Sep 12, 2024 · US
Urethanases for the enzymatic degradation of polyurethanes
US12351853B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12351853-B2 |
| Application number | US-202217692254-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 11, 2022 |
| Priority date | Jun 21, 2018 |
| Publication date | Jul 8, 2025 |
| Grant date | Jul 8, 2025 |
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- Title
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Abstract
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The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method comprising utilizing a polypeptide for cleaving a urethane group, the polypeptide having an amino acid sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 7, and variants of said polypeptides, characterized in that the polypeptide has urethanase activity in the enzymatic cleavage of urethane linkages, wherein variants is defined as an amino acid sequence having at least 90% sequence identity with a polypeptide to which it refers. 2. The method according to claim 1 , wherein the urethane group is aromatically attached, wherein aromatically attached is defined as a nitrogen atom of the urethane group being directly attached to an aromatic ring. 3. The method according to claim 1 , wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 7, and variants of said polypeptides, wherein variants is defined as an amino acid sequence having at least 95% sequence identity with a polypeptide to which it refers. 4. The method according to claim 1 , wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, and SEQ ID No. 7. 5. The method according to claim 1 , wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10. 6. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 7 or a variant thereof. 7. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 2 or a variant thereof. 8. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 3 or a variant thereof. 9. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 4 or a variant thereof. 10. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 5 or a variant thereof. 11. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 6 or a variant thereof. 12. The method according to claim 1 , wherein the polypeptide has an amino acid sequence in accordance with SEQ ID No. 8 or a variant thereof.
Assignees
Inventors
Classifications
Plastics recycling; Rubber recycling · CPC title
Urethanase (3.5.1.75) · CPC title
polyhydric · CPC title
- C12N9/80Primary
acting on amide bonds in linear amides {(3.5.1)} · CPC title
from polyesters · CPC title
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Frequently asked questions
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- What does patent US12351853B2 cover?
- The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.
- Who is the assignee on this patent?
- Covestro Intellectual Property Gmbh & Co Kg, Covestro Deutschland Ag
- What technology area does this patent fall under?
- Primary CPC classification C12N9/80. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 08 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).