Use of ganoderma fungal RNA molecules, drug, drug composition and delivery carrier

Patent metadata

Field	Value
Publication number	US-12351801-B2
Application number	US-202218260789-A
Country	US
Kind code	B2
Filing date	Nov 17, 2022
Priority date	Mar 3, 2022
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

A use of Ganoderma fungal RNA molecules, a drug, a drug composition and a delivery carrier are provided. It has been proved that the tRFs derived from the Ganoderma traditional Chinese medicine has an obvious inhibiting effect on airway smooth muscle cell phenotype change (proliferation, abnormal secretion and the like) induced by TGF-β1 and AHR, inflammation and remodeling of asthma mice induced by Dermatophagoides pteronyssinus in the experiments of the present disclosure, which suggests that the tRFs derived from the Ganoderma traditional Chinese medicine and a pharmaceutical composition can be a brand new medicine for treating asthma. The drug comprises the RNA molecule and a pharmaceutically acceptable carrier, diluent and/or excipient. The present disclosure is helpful for the research and development of asthma treatment drugs and provides reference for the research and development of other respiratory system disease treatment drugs.

First claim

What is claimed is:

1. A Ganoderma fungal RNA molecule, which is used in preparation of asthma prevention or treatment drugs,
wherein the Ganoderma fungal RNA molecule originates from transfer RNA molecule fragments (tRNA fragment, tRFs) of Ganoderma traditional Chinese medicine,
wherein a sequence of the tRFs of Ganoderma traditional Chinese medicine comprises a sequence selected from SEQ ID NO. 4 to NO. 6.

2. The Ganoderma fungal RNA molecule according to claim 1 , wherein the tRFs of Ganoderma traditional Chinese medicine are double stranded RNA molecules, the tRFs of Ganoderma traditional Chinese medicine comprise an antisense sequence selected from SEQ ID NO. 4 to NO. 6 and a complementary sense sequence selected from SEQ ID NO. 10 to NO. 12.

3. The Ganoderma fungal RNA molecule according to claim 2 , wherein the double stranded RNA molecules further comprise a 3′ suspension.

4. The Ganoderma fungal RNA molecule according to claim 3 , wherein a nucleotide sequence of an antisense and/or sense strand of the double stranded RNA molecules comprises at least one chemically modified nucleotide; wherein the chemical modification is selected from at least one of 1-methyl, 2-methyl, 5-methyl, 7-methyl, N2-methyl, N6-methyl, N2,N2-dimethyl, 2′-O-methyl, N6-isopentenyl, 2-methylthio-N6-isopentenyl, N6-threonic carbamoyl, N6-methyl-N6-threonic carbamoyl, 2-thio, 4-thio, N4-acetyl, 5-formyl, 3-(3-amino-3-carboxypropyl), 5-methoxy, 5-oxyacetic acid, 5-oxyacetic acid methyl ester, 5-methoxycarbonylmethyl, 5-methoxycarbonylmethyl-2′-O-methyl, 5-methoxycarbonylmethyl-2-thio, 5-aminomethyl-2-thio, 5-methylaminomethyl, 5-methylaminomethyl-2-thio, 5-aminoformylmethyl, 5-aminoformylmethyl-2′-O-methyl, 5-carboxymethylaminomethyl, 5-carboxymethylaminomethyl-2′-O-methyl, 5-carboxymethylaminomethyl-2-methyl, 5-taurine methyl, and 5-taurinemethyl-2-thio.

5. A drug for preventing or treating asthma, wherein an active ingredient comprises at least one of the transfer RNA molecule fragments of Ganoderma traditional Chinese medicine according to claim 1 , and an optionally a pharmaceutically acceptable carrier, a diluent and/or an excipient.

6. The drug according to claim 5 , wherein the drug further comprises a nucleic acid stabilizer.

7. A delivery carrier for preventing and treating asthma, comprising the double stranded RNA molecules according to claim 3 , and an optionally a pharmaceutically acceptable carrier, a diluent and/or an excipient.

Assignees

• Univ Macau Sci & Tech

Inventors

• Jiang Zhihong
• Ma Yuan
• Ren Fei

Classifications

• A61K36/074

  Ganoderma · CPC title

• A61P11/06

  Antiasthmatics · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title

• C12N2320/30

  Special therapeutic applications · CPC title

• C12N2310/18

  acting by a non-sequence specific mechanism · CPC title