Assay with rapid temperature change

Patent metadata

Field	Value
Publication number	US-12350680-B2
Application number	US-201816484998-A
Country	US
Kind code	B2
Filing date	Feb 15, 2018
Priority date	Feb 15, 2017
Publication date	Jul 8, 2025
Grant date	Jul 8, 2025

Abstract

The present invention provides devices, systems, and methods for rapid and easy-to-use in sample thermal cycling or temperature changes for the facilitation of reactions such as but not limited to PCR.

First claim

The invention claimed is:

1. A device for changing temperature of a thin fluidic sample, comprising:
a first plate, a second plate, spacers, and a clamp, wherein:
(i) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
(ii) each of the plates comprises, on its respective inner surface, a sample contact area for contacting a fluidic sample;
(iii) one or both of the plates comprise the spacers on the inner surface thereof; and
(iv) the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, wherein at least one of the spacers is inside the sample contact area
wherein in an open configuration, the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers;
wherein in a closed configuration, the two plates are operable to compress at least a part of the sample into a layer of substantially uniform thickness that is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
wherein the inner surface of the first plate faces the inner surface of the second plate in the closed configuration;
wherein the clamp compresses the first plate and the second plate to fix the two plates together in the closed configuration, wherein the pressure of the clamp applied on the plates is 0.01 kg/cm 2 or higher;
wherein at least one of the first and second plates comprise a radiation absorbing layer that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s; and
wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array.

2. A device for changing temperature of a thin layer of a fluidic sample, comprising:
a first plate, a second plate, and spacers, wherein:
(i) the first plate has a thickness of 100 um (micron) or less;
(ii) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
(iii) each of the plates comprises, on its respective inner surface, a sample contact area for contacting a fluidic sample;
(iv) one or both of the plates comprise the spacers that are fixed to the respective inner surface thereof;
(v) the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns;
(vi) at least one of the spacers is inside the sample contact area;
wherein in an open configuration, the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers; and
wherein in a closed configuration, which is configured after the sample is deposited in the open configuration, at least a part of the sample is compressed by the two plates into a layer of substantially uniform thickness and is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
wherein at least one of the first and second plates comprise a radiation absorbing layer that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s; and
wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array.

3. A device for changing temperature of a thin layer of a fluidic sample, comprising:
a first plate, a second plate, and a clamp, wherein:
(i) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
(ii) each of the plates comprises, on its respective surface, a sample contact area for contacting a fluidic sample to be assayed; and
(iii) the clamp compresses the first plate and the second plate to fix the plates at the closed configuration, wherein the pressure of the clamp applied on the plates is 0.01 kg/cm 2 or higher,
wherein at least one of the first and second plates comprise a radiation absorbing layer that that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s;
wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array;
wherein in an open configuration, the two plates are partially or completely separated apart, the average spacing between the plates is 250 μm or larger; and
wherein in a closed configuration, which is configured after the sample is deposited in the open configuration, at least a part of the sample is compressed by the two plates into a layer of substantially uniform thickness and is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is 200 μm thick or less.

4. The device of claim 1 , wherein the radiation absorbing layer is near the at least part of the sample, whereas the area of the at least part of the sample and the radiation absorbing layer are substantially larger than the layer.

5. The device of claim 4 , wherein the area of the at least part of the sample and the radiation absorbing layer are substantially larger than the uniform thickness of the sample.

6. The device of claim 1 , wherein one of the first and second plates has a thickness of 100 μm or less.

7. The device of claim 1 , wherein the radiation absorbing layer is near the at least part, wherein one of the first and second plates of a thickness of 100 μm or less.

8. The device of claim 2 , wherein the clamp compresses the first plate and the second plate together in the closed configuration, wherein the pressure of the clamp applied on the plates is 0.01 kg/cm 2 or higher.

9. The device of claim 2 , wherein the clamp that compresses the first plate and the second plate together in the closed configuration, and wherein the pressure of the clamp applied on the plates is 0.01 kg/cm 2 or higher.

10. A system for changing temperature of a thin fluidic sample layer, comprising:
(i) the device of claim 1 ,
(ii) a radiation source, wherein the radiation source is configured to radiate electromagnetic waves that the radiation absorbing layer absorbs significantly; and
(iii) a controller that is configured to control the radiation source and change the temperature of the sample.

11. A method for changing temperature of a layer of a fluidic sample, comprising:
(i) providing the system of claim 10 ;
(ii) depositing the fluid sample on one or both of the plates of the device when the plates are in the open configuration;
(iii) after (ii), pressing the plates into the closed configuration wherein the plates compress at least a part of the sample into the layer of a substantially uniform thickness less than 200 μm; and
(iv) changing and maintaining the temperature of the layer by changing the presence, intensity, wavelength, frequency, and/or angle of the electromagnetic waves from the radiation source.

12. The device of claim 1 , wherein the clamp is configured to comprise a heat insulator layer to reduce the heat conduction between the clamp and the plates, and wherein the heat insulat

Assignees

• Essenlix Corp

Inventors

• Chou Stephen Y
• Ding Wei
• Zhang Yufan
• Qi Ji

Classifications

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• B01L2300/1883

  using thermal insulation · CPC title

• B01L2300/1811

  using electromagnetic induction heating · CPC title

• B01L2300/12

  Specific details about materials · CPC title

• B01L2300/0809

  rectangular shaped · CPC title