DNA ligase variants
US-10837009-B1 · Nov 17, 2020 · US
US12344872B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12344872-B2 |
| Application number | US-201815972919-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 7, 2018 |
| Priority date | May 8, 2017 |
| Publication date | Jul 1, 2025 |
| Grant date | Jul 1, 2025 |
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The present invention provides engineered ligase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered ligase polypeptides. The invention also provides methods for use of the compositions comprising the engineered ligase polypeptides for diagnostic and other purposes.
Opening claim text (preview).
What is claimed is: 1. An engineered T4 DNA ligase comprising a polypeptide sequence, wherein the polypeptide sequence consists of SEQ ID NO:38.
DNA ligase (ATP) (6.5.1.1) · CPC title
Polynucleotides, e.g. nucleic acids, oligoribonucleotides · CPC title
Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title
Massive parallel sequencing · CPC title
incorporating an adaptor · CPC title
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