Bioreactor insert and biofilm support, related apparatus and related methods

Patent metadata

Field	Value
Publication number	US-12344830-B2
Application number	US-202418623416-A
Country	US
Kind code	B2
Filing date	Apr 1, 2024
Priority date	Oct 1, 2018
Publication date	Jul 1, 2025
Grant date	Jul 1, 2025

Abstract

The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

First claim

What is claimed:

1. A method for forming a bioreactor product, the method comprising:
providing a bioreactor comprising:
a reaction vessel defining an interior reaction volume; and
a bioreactor insert apparatus mounted within the reaction vessel, the bioreactor insert apparatus comprising:
an inlet volume in fluid communication with a first fluid inlet;
an outlet volume in fluid communication with a first fluid outlet;
a plurality of exit volumes, each exit volume being in fluid communication with the inlet volume and comprising at least one exit biofilm support disposed at a boundary between the exit volume and an external volume outside the bioreactor insert apparatus, wherein: the exit biofilm support is adapted to promote growth, attachment, and metabolism of microorganisms in the form of a biofilm thereon, and the exit biofilm support is adapted to permit fluid and solid transport across the exit biofilm support and between the exit volume and the external volume; and
a plurality of recirculation volumes, each recirculation volume being in fluid communication with the outlet volume and comprising at least one recirculation biofilm support disposed at a boundary between the recirculation volume and the external volume outside the bioreactor insert apparatus, wherein: the recirculation biofilm support is adapted to promote growth, attachment, and metabolism of microorganisms in the form of a biofilm thereon, and the recirculation biofilm support is adapted to permit fluid and solid transport across the recirculation biofilm support and between the recirculation volume and the external volume;

wherein:
the external volume corresponds to a portion of the interior reaction volume outside the bioreactor insert apparatus,
the bioreactor insert apparatus further comprises biofilms adhered to the biofilm supports, the biofilms having biological activity,
an aqueous reaction medium at least partially fills the interior reaction volume and the bioreactor insert apparatus, and
suspended microorganisms are present in the aqueous reaction medium;

feeding an influent stream comprising one or more reactants for conversion to the bioreactor insert apparatus via the first fluid inlet;
circulating the influent stream through the inlet volume, into the exit volumes, through the exit biofilm supports and biofilms thereon, into the external volume, through the recirculation biofilm supports and biofilms thereon, into the recirculation volumes, into the outlet volume, and through the first fluid outlet; and
converting the one or more influent reactants to a product.

2. The method of claim 1 , wherein at least 50 % of total microorganisms in the bioreactor are incorporated into the biofilms.

3. The method of claim 1 , wherein the biofilms have methanogenic activity.

4. The method of claim 3 , wherein aqueous fluid removed through the first fluid outlet comprises dissolved methane at a concentration in a range of 50% to 150% relative to the equilibrium concentration of methane in water.

5. The method of claim 1 , wherein the biofilms comprising a community of anaerobic microorganisms.

6. The method of claim 1 , wherein the biofilms comprising a community of aerobic microorganisms.

7. The method of claim 1 , wherein the biofilms comprising a community of anoxic microorganisms.

8. The method of claim 1 , comprising operating the bioreactor at a temperature in a range of 1° C. to 60° C.

9. The method of claim 1 , where the bioreactor further comprises attachment media for microbial growth suspended in the aqueous reaction medium.

10. The method of claim 1 , comprising operating the bioreactor at a hydraulic retention time (HRT) in a range of 2 hr to 40 hr.

11. The method of claim 1 , wherein:
the influent stream comprises at least one of municipal waste and municipal wastewater; and
the at least one of the municipal waste and the municipal wastewater comprises at least one of organic constituents and microorganism nutrients that are removed from the influent stream.

12. The method of claim 1 , wherein:
the influent stream comprises at least one of agricultural waste and agricultural wastewater; and
the at least one of the agricultural waste and the agricultural wastewater comprises at least one of organic constituents and microorganism nutrients that are removed from the influent stream.

13. The method of claim 1 , wherein:
the influent stream comprises at least one of dairy waste and dairy wastewater; and
the at least one of the dairy waste and the dairy wastewater comprises at least one of organic constituents and microorganism nutrients that are removed from the influent stream.

14. The method of claim 1 , wherein:
the influent stream comprises at least one of food and beverage waste and food and beverage wastewater; and
the at least one of the food and beverage waste and the food and beverage wastewater comprises at least one of organic constituents and microorganism nutrients that are removed from the influent stream.

15. The method of claim 1 , wherein:
the influent stream comprises at least one of paper waste and paper wastewater; and
the at least one of the paper waste and the paper wastewater comprises at least one of organic constituents and microorganism nutrients that are removed from the influent stream.

16. The method of claim 1 , wherein the product comprises a fermentation product.

17. The method of claim 1 , wherein the product comprises one or more organic acids.

18. The method of claim 17 , wherein the one or more organic acids comprises one or more volatile organic acids.

19. The method of claim 17 , wherein the one or more organic acids comprises one or more non-volatile organic acids.

20. The method of claim 1 , wherein the influent stream comprises particulate solid material.

21. The method of claim 1 , wherein the one or more reactants comprises at least one organic constituent selected from the group consisting of proteins, lipids, carbohydrates, and combinations thereof.

22. The method of claim 1 , wherein:
the product comprises a gas product stream comprising (i) 60-95% methane relative to the gas product stream and (ii) at least one of carbon dioxide and hydrogen;
the method further comprises recovering the gas product stream.

23. The method of claim 1 , further comprising:
periodically reversing a flow direction of the influent stream through the bioreactor insert apparatus.

Assignees

• Univ Michigan Regents

Inventors

• Skerlos Steven
• Raskin Lutgarde
• Fairley-Wax Timothy
• Jalgaonkar Nishant

Classifications

• C12P5/023

  Methane · CPC title

• C12M33/14

  with filters, sieves or membranes · CPC title

• C12M27/14

  Rotation or movement of the cells support, e.g. rotated hollow fibers · CPC title

• C12M25/00

  Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings · CPC title

• C12M23/06

  Tubular (C12M23/08, C12M23/16 take precedence) · CPC title