Use of natural attapulgite as natural nano mineral enzyme

Patent metadata

Field	Value
Publication number	US-12337299-B2
Application number	US-202117608333-A
Country	US
Kind code	B2
Filing date	Jan 15, 2021
Priority date	Jan 16, 2020
Publication date	Jun 24, 2025
Grant date	Jun 24, 2025

Abstract

A method for using a natural attapulgite is disclosed. The method includes using the natural attapulgite as a natural nano mineral enzyme. The results of the examples show that the natural attapulgite has peroxidase-like activity, catalase-like activity or superoxide dismutase-like activity, and good biocompatibility. Compared with protease, the natural attapulgite has the advantages such as large reserves, easy to obtain, low cost, high temperature resistance and wide range of pH value. Compared with a developed artificial nano enzymes, the natural attapulgite further has the advantages such as multi-function, natural non-toxic (from nature, no heavy metals), good biocompatibility, easy to obtain, no complex processing, and huge surface area which provides a place for cell growth and proliferation.

First claim

What is claimed is:

1. A method for using a natural attapulgite comprising:
mixing a natural attapulgite dispersion, an H 2 O 2 solution, a substrate solution and a buffer solution to obtain a mixture, and subjecting the mixture to enzyme catalysis; wherein
the natural attapulgite dispersion consists of the natural attapulgite and water, and the natural attapulgite dispersion has a concentration of the natural attapulgite of 1-5 mg/mL; and
the natural attapulgite is a one-dimensional fibrous hydrated magnesium aluminosilicate clay mineral.

2. The method of claim 1 , wherein the buffer solution is HAc-NaAc or a phosphate buffer solution, and has a pH value of 2-10.

3. The method of claim 1 , wherein the H 2 O 2 solution has a mass concentration of 30%.

4. The method of claim 1 , wherein the buffer solution has a concentration of 0.1 M and a pH value of 7.0.

5. A method for using a natural attapulgite comprising:
adding 0.4 mL of a natural attapulgite dispersion with a concentration of 2.5 mg/mL into 5.0 mL of a buffer solution, and adding 100 μL of an H 2 O 2 solution thereto, and measuring a solubility of O 2 produced at different reaction time; wherein
the natural attapulgite dispersion consists of the natural attapulgite and water; and
the natural attapulgite is a one-dimensional fibrous hydrated magnesium aluminosilicate clay mineral.

Assignees

• Univ China Geosciences Beijing

Inventors

• Zhang Yihe
• Liang Minmin
• Feng Feng
• Wang Peixia
• Tong Wangshu
• An Qi

Classifications

• B01J35/27

  in a liquid or molten state · CPC title

• C01B13/0207

  Water · CPC title

• B01J37/343

  of ultrasonic wave energy · CPC title

• B01J37/0036

  Grinding · CPC title

• B01J35/615

  100-500 m2/g · CPC title