Compositions and methods for accurately identifying mutations
US-2024409996-A1 · Dec 12, 2024 · US
Fungal autoinducible expression system
US12331302B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12331302-B2 |
| Application number | US-202017092031-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 6, 2020 |
| Priority date | Nov 8, 2019 |
| Publication date | Jun 17, 2025 |
| Grant date | Jun 17, 2025 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention provides for a system comprising: (a) a first nucleic acid encoding an α-factor receptor operatively linked to a first promoter, (b) a second nucleic acid encoding a recombinase operatively linked to a promoter which is activated by an α-factor receptor bound to an α-factor, and (c) a third nucleic acid encoding a gene of interest (GOI) flanked by a pair of recombinase recognition sequences, recognized by the recombinase, operatively linked to a second promoter. The present invention provides for a genetically modified fungal cell comprising the system of the present invention.
First claim
Opening claim text (preview).
What is claimed is: 1. A system comprising: (a) a first nucleic acid encoding an α-factor receptor operatively linked to a first promoter, (b) a second nucleic acid encoding a recombinase operatively linked to a promoter which is activated by the α-factor receptor bound to a yeast α-factor, and (c) a third nucleic acid comprising a gene of interest (GOI) flanked by a pair of recombinase recognition sequences, recognized by the recombinase, operatively linked to a second promoter; wherein the yeast α-factor is MFα1 gene product and the α-factor receptor is STE2, and the recombinase is one selected from the following: # Name Host 1 BSu_xerC Bacillus subtilis 2 BSu_xerD Bacillus subtilis 3 BSu_ydcL Bacillus subtilis 4 CBu_tnpA Clostridium butyricum 5 Col1D Escherichia coli 6 CP4-57 Escherichia coli 7 Cre Escherichia coli 8 D29 Mycobacterium smegmatis 9 DLP12 Escherichia coli 10 DNo_int Dichelobacter nodosus 11 ECo_fimB Escherichia coli 12 ECo_fimE Escherichia coli 13 ECo_orf Escherichia coli 14 ECo_xerC Escherichia coli 15 ECo_xerD Escherichia coli 16 HIn_orf Haemophilus influenzae 17 HIn_rci Haemophilus influenzae 18 HIn_xerC Haemophilus influenzae 19 HIn_xerD Haemophilus influenzae 20 HK22 Escherichia coli 21 HP1 Haemophilus influenzae 22 L2 Acholeplasma sp. 23 L5 Mycobacterium tuberculosis 24 L54 Staphylococcus aureus 25 Lambda Escherichia coli 26 LLe_orf Lactobacillus leichmannii 27 LLe_xerC Lactobacillus leichmannii 28 phi10MC Oenococcus oeni 29 MJa_orf Methanococcus jannaschi 30 MLe_xerD Mycobacterium leprae 31 MPa_int Mycobacterium paratuberculosis 32 MTu_int Mycobacterium tuberculosis 33 MTu_xerC Mycobacterium tuberculosis 34 MV4 Lactobacillus delbrueckii 35 MX8 Myxococcus xanthus 36 pAE1 Alcaligenes eutrophus 37 pCL1 Chlorobium limicola 38 pDU1 Nostoc sp. 39 pMEA Amycolatopsis methanolica 40 RSp_EF Rhizobium sp. 41 RSp_GC Rhizobium sp. 42 RSp_QK Rhizobium sp. 43 RSp_RA Rhizobium sp. 44 RSp_RB
Assignees
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Classifications
Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title
inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor · CPC title
for yeast · CPC title
having a known sequence of two or more amino acids, e.g. glutathione · CPC title
from Saccharomyces · CPC title
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- What does patent US12331302B2 cover?
- The present invention provides for a system comprising: (a) a first nucleic acid encoding an α-factor receptor operatively linked to a first promoter, (b) a second nucleic acid encoding a recombinase operatively linked to a promoter which is activated by an α-factor receptor bound to an α-factor, and (c) a third nucleic acid encoding a gene of interest (GOI) flanked by a pair of recombinase rec…
- Who is the assignee on this patent?
- Zargar Amin, Landberg Jenny, Keasling Jay D, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/81. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 17 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).