Feed ingredients comprising lysed microbial cells

Patent metadata

Field	Value
Publication number	US-12329183-B2
Application number	US-202217864768-A
Country	US
Kind code	B2
Filing date	Jul 14, 2022
Priority date	Jul 1, 2016
Publication date	Jun 17, 2025
Grant date	Jun 17, 2025

Abstract

The present disclosure relate to feed ingredients and formulated feed, methods for their manufacture, and uses thereof in nutritional applications such as in aquaculture, terrestrial animal feed, and human nutrition. The feed ingredient compositions comprise lysed microbial cells with a small aspect ratio and triglyceride oil.

First claim

What is claimed:

1. A formulated feed comprising edible food and at least 20%, by weight of the formulated feed, of a feed ingredient composition comprising a dispersion of lysed microalgal cells in triglyceride oil, wherein:
a. 5-90% by weight of the feed ingredient composition is lysed microalgal cells from the family Thraustochytriaceae or the genus Crypthecodinium , and
b. 10-95% by weight of the feed ingredient composition is triglyceride oil, wherein the triglyceride oil comprises oil from the lysed microalgal cells and oil from another organism, and
wherein the lysed microalgal cells have a median particle size Dv50 of from 5-50 micrometers.

2. The formulated feed according to claim 1 , wherein the lysed microalgal cells have a median particle size Dv50 of from 5-20 micrometers.

3. The formulated feed according to claim 2 , wherein the lysed microalgal cells have a median particle size Dv50 of from5-15 micrometers.

4. The formulated feed according to claim 1 , wherein greater than half of the lysed microalgal cells remain suspended in the composition for at least a week without settling.

5. The formulated feed according to claim 1 , wherein the triglyceride oil from another organism is oil from a fish, a plant selected from the group consisting of coconut, com, cottonseed, olive, peanut, rapeseed, canola, safflower, sesame, nut, camelina, citrus, and combinations thereof, an oleaginous microbe, an animal, or combinations thereof.

6. The formulated feed according to claim 1 , wherein the feed ingredient composition is comprised of 12-45% by weight of docosahexaenoic acid (DHA).

7. The formulated feed according to claim 1 , wherein the lysed microalgal cells are of the genus Crypthecodinium, Thraustochytriuni, Aurantiochytrium , or Schizochytrium.

8. The formulated feed according to claim 1 , wherein the triglyceride oil has a fatty acid profile of 10-70% DHA by weight of fatty acids.

9. The formulated feed according to claim 1 , wherein the oil from the lysed microalgal cells has a fatty acid profile of 50-70% DHA by weight of fatty acids.

10. The formulated feed according to claim 1 , wherein combustion of the feed ingredient composition does not occur for at least 20 hours when the feed is subjected to 80° C.

11. The formulated feed according to claim 1 , wherein the feed ingredient composition is prepared by:
a. blending microalgal cells from the family Thraustochytriaceae or the genus Crypthecodinium and the oil from another organism to form a blend, and
b. lysing the microalgal cells in the blend to form the composition as a dispersion.

12. The method of claim 11 , wherein the lysing comprises bead milling.

13. The formulated feed according to claim 1 , wherein the edible food is an aquaculture feed or animal feed.

14. The formulated feed according to claim 1 , wherein the edible food is a salmon feed.

15. The formulated feed according to claim 1 , wherein the formulated feed is coated with the feed ingredient composition.

16. A method for preparing the formulated feed of claim 1 , comprising contacting the feed ingredient composition with an edible food.

17. The method according to claim 16 , wherein the contacting comprises coating the edible food with the feed ingredient composition.

18. A method comprising feeding farmed fish or shell fish with the formulated feed of claim 1 .

Assignees

• Corbion Biotech Inc
• Caravan Ingredients Inc

Inventors

• Rakitsky Walter
• Piechocki John
• Lu Wenhua

Classifications

• A23K50/80 Primary

  for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title

• A23K40/30

  by encapsulating; by coating · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title

• A23K10/16

  Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence) · CPC title

• B65D85/00 Primary

  Containers, packaging elements or packages, specially adapted for particular articles or materials (bundles of articles held together by packaging elements for convenience of storage or transport B65D71/00) · CPC title