Modular assemblies and systems for cell cultures and methods thereof
US-10988723-B1 · Apr 27, 2021 · US
Open-top microfluidic devices and methods for simulating a function of a tissue
US12325846B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12325846-B2 |
| Application number | US-202418604469-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 13, 2024 |
| Priority date | Dec 4, 2015 |
| Publication date | Jun 10, 2025 |
| Grant date | Jun 10, 2025 |
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- Title
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- Abstract
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- First independent claim
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Abstract
Official abstract text for this publication.
A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of seeding cells, comprising: (a) providing a microfluidic device comprising a first chamber, the first chamber including a three-dimensional gel matrix disposed therein, and a second chamber, said second chamber separated from the first chamber by a membrane, the gel matrix positioned under a removable cover that comprises a fluidic chamber, wherein said fluidic chamber of said removable cover substantially aligns with all of an open top region of the first chamber; (b) removing the removable cover thereby exposing the gel matrix; and (c) seeding cells within the gel matrix. 2. The method of claim 1 , wherein the gel matrix has a patterned surface. 3. The method of claim 1 , wherein the membrane includes endothelial cells. 4. The method of claim 1 , wherein the second chamber includes fluidics configured to supply the cells with culture fluid. 5. The method of claim 1 , further comprising (d) culturing the cells submerged in culture fluid. 6. The method of claim 5 , further comprising (e) culturing the cells in air without culture media. 7. The method of claim 1 , wherein said cells are intestinal cells.
Assignees
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Classifications
Vascular Endothelial cells · CPC title
Cells of the gastro-intestinal tract · CPC title
Adult fibroblasts · CPC title
Keratinocytes; Whole skin · CPC title
of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title
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Frequently asked questions
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- What does patent US12325846B2 cover?
- A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes …
- Who is the assignee on this patent?
- Harvard College
- What technology area does this patent fall under?
- Primary CPC classification C12M21/08. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 10 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).