Application of heptoglycan-chain-containing oligosaccharide compounds in preparation of vaccine against Helicobacter pylori

Patent metadata

Field	Value
Publication number	US-12324832-B2
Application number	US-202117391061-A
Country	US
Kind code	B2
Filing date	Aug 2, 2021
Priority date	Apr 13, 2020
Publication date	Jun 10, 2025
Grant date	Jun 10, 2025

Abstract

Disclosed is the application of heptoglycan-chain-containing oligosaccharide compounds in preparation of a vaccine against Helicobacter pylori , belonging to the field of medicine. Different antigen oligosaccharides were obtained by chemical synthesis and fixed onto a chip surface to prepare a synthetic oligosaccharide chip. With animal immunization experiments and synthetic oligosaccharide chip analysis, the antibodies in antiserum produced by Helicobacter pylori polysaccharide immunization are used to determine the structure-activity relationship between the synthetic oligosaccharides and immunogenicity. It is found that the heptoglycan chain in the oligosaccharide compounds is an important immune epitope, and oligosaccharides containing α-(1→3) heptoglycan chain can be used in preparation and development of vaccines for prevention and treatment of Helicobacter pylori infection.

First claim

What is claimed is:

1. A method for preparing a vaccine against Helicobacter pylori , comprising the step of incorporating a heptoglycan-chain-containing oligosaccharide of the structure (I) as an active component in the vaccine formulation

2. The method of claim 1 , wherein a synthesis process of the heptoglycan-chain-containing oligosaccharide is as follows:

3. An oligosaccharide chip, wherein the oligosaccharide chip is prepared by fixing the heptoglycan-chain-containing oligosaccharide of claim 1 onto a chip surface through an amino linker of the oligosaccharide.

4. A vaccine for the prevention and treatment of Helicobacter pylori infection, comprising the heptoglycan-chain-containing oligosaccharide of claim 1 as an active component.

Assignees

• Hu Jing
• Yin Jian
• Univ Jiangnan

Inventors

• Hu Jing
• Yin Jian

Classifications

• C07H3/06 Primary

  Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages · CPC title

• C07H1/00

  Processes for the preparation of sugar derivatives · CPC title

• A61K39/105 Primary

  Delta proteobacteriales, e.g. Lawsonia; Epsilon proteobacteriales, e.g. campylobacter, helicobacter · CPC title

• A61P1/04

  for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants · CPC title

• A61P31/04

  Antibacterial agents · CPC title