Nucleic acid analysis by joining barcoded polynucleotide probes

Patent metadata

Field	Value
Publication number	US-12319957-B2
Application number	US-202117458995-A
Country	US
Kind code	B2
Filing date	Aug 27, 2021
Priority date	Sep 8, 2015
Publication date	Jun 3, 2025
Grant date	Jun 3, 2025

Abstract

Disclosed are compositions, methods and kits for determining the presence, absence, amount, copy number, or other characteristics of one or more polynucleotide sequences in two or more samples and use thereof in genotyping, evaluation of copy number variation, expression analysis, determination of splice variants and fusion genes, and other genetic analyses.

First claim

The invention claimed is:

1. A composition for analyzing a target polynucleotide in a sample, the composition comprising:
a first probe configured to hybridize to a first target sequence of the target polynucleotide, the first probe including
(i) an interrogation site bar code that is non-complementary to the first target sequence,
(ii) a first complementary sequence portion 5′ of the interrogation site barcode, the first complementary sequence portion being complementary to the first target sequence,
(iii) a second complementary sequence portion 3′ of the interrogation site barcode, the second complementary sequence portion being complementary to the first target sequence, and
(iv) a universal sequence that is non-complementary to the first target sequence, the universal sequence being 5′ of the first complementary sequence portion; and
a second probe configured to hybridize to a second target sequence of the target polynucleotide, the second probe including
(i) a complementary sequence portion that is complementary to the second target sequence, and
(ii) a non-complementary sequence that is non-complementary to the second target sequence.

2. The composition of claim 1 , wherein the non-complementary sequence of the second probe and the complementary sequence of the second probe are immediately adjacent one another.

3. The composition of claim 2 , wherein the non-complementary sequence of the second probe is 3′ of the complementary sequence of the second probe.

4. The composition of claim 1 , wherein the non-complementary sequence of the second probe comprises a universal sequence.

5. The composition of claim 4 , wherein the universal sequence of the second probe and the universal sequence of the first probe are the same.

6. The composition of claim 1 , wherein the 3′ end of the first probe is complementary to one form of a single nucleotide polymorphism (SNP) or other genetic variation.

7. The composition of claim 1 , wherein the first and second probes are configured to be adjacent one another when hybridized to the target polynucleotide.

8. The composition of claim 1 , wherein the first and second probes are configured to be spaced by 1 to 500 nucleotides when hybridized to the target polynucleotide.

9. The composition of claim 1 , wherein the interrogation site bar code is 10-16 nucleotides in length.

10. A composition for analyzing a target polynucleotide in a sample, the composition comprising:
a first probe configured to hybridize to a first target sequence of the target polynucleotide, the first probe including
(v) an interrogation site bar code that is non-complementary to the first target sequence,
(vi) a first complementary sequence portion 5′ of the interrogation site barcode, the first complementary sequence portion being complementary to the first target sequence,
(vii) a second complementary sequence portion 3′ of the interrogation site barcode, the second complementary sequence portion being complementary to the first target sequence, and
(viii) a universal sequence that is non-complementary to the first target sequence; and
a second probe configured to hybridize to a second target sequence of the target polynucleotide, the second probe including
(iii) a complementary sequence portion that is complementary to the second target sequence, and
(iv) a non-complementary sequence that is non-complementary to the second target sequence,
wherein the first probe is configured to hybridize to the target polynucleotide 5′ of the second probe.

11. The composition of claim 10 , wherein the non-complementary sequence of the second probe and the complementary sequence of the second probe are immediately adjacent one another.

12. The composition of claim 11 , wherein the non-complementary sequence of the second probe is 3′ of the complementary sequence of the second probe.

13. The composition of claim 10 , wherein the non-complementary sequence of the second probe comprises a universal sequence.

14. The composition of claim 13 , wherein the universal sequence of the second probe and the universal sequence of the first probe are the same.

15. The composition of claim 10 , wherein the 3′ end of the first probe is complementary to one form of a single nucleotide polymorphism (SNP) or other genetic variation.

16. The composition of claim 10 , wherein the first and second probes are configured to be adjacent one another when hybridized to the target polynucleotide.

17. The composition of claim 10 , wherein the first and second probes are configured to be spaced by 1 to 500 nucleotides when hybridized to the target polynucleotide.

18. The composition of claim 10 , wherein the interrogation site bar code is 10-16 nucleotides in length.

19. A kit for analyzing a target polynucleotide in a sample, the kit comprising:
the composition of claim 1 ; and
a set of primers, at least one of which is a universal primer complementary to the universal sequence of the first probe.

20. The kit of claim 19 , further comprising an additional sequence that includes one or more of: a sample index; an adaptor for next generation sequencing; or a capture probe or sequence, wherein the set of primers is configured to add the additional sequence.

Assignees

• Affymetrix Inc

Inventors

• Koshinsky Heather
• Curry John D
• O'Callahan Robert
• Mccoy Adam
• Fitzpatrick Daniel
• Dickinson Philip H
• Schweitzer Anthony C

Classifications

• C12Q1/6862

  Ligase chain reaction [LCR] · CPC title

• C12Q1/6853

  using modified primers or templates · CPC title

• C12Q2525/204

  specific length of the oligonucleotides · CPC title

• C12Q2525/161

  incorporating target specific and non-target specific sites · CPC title

• C12Q2525/155

  incorporating/generating a new priming site · CPC title