Method and an apparatus for an enzymatic hydrolysis, a liquid fraction and a solid fraction

Patent metadata

Field	Value
Publication number	US-12319950-B2
Application number	US-202117326442-A
Country	US
Kind code	B2
Filing date	May 21, 2021
Priority date	Jun 3, 2016
Publication date	Jun 3, 2025
Grant date	Jun 3, 2025

Abstract

In a method and an apparatus for an enzymatic hydrolysis in which plant based raw material is hydrolysed by means of enzymes in at least one enzymatic hydrolysis stage. A plant based feed ( 1 ) is fed to the enzymatic hydrolysis stage ( 2 ) in which the plant based feed is hydrolysed. A liquid fraction ( 3 ) comprising carbohydrates is separated from a solid fraction ( 4 ) in a solid-liquid separation stage ( 11 ). At least a part ( 5 ) of the solid fraction ( 4 ) comprising enzymes is recirculated to the plant based feed ( 1 ) of the enzymatic hydrolysis stage ( 2 ) or to the enzymatic hydrolysis stage ( 2 ), and a rest part ( 6 ) of the solid fraction ( 4 ) is recovered. Further, the invention relates to the liquid fraction and the solid fraction and their use.

First claim

The invention claimed is:

1. A method for an enzymatic hydrolysis in which plant based material is hydrolysed using enzymes in at least two enzymatic hydrolysis stages, the method comprising:
pretreating wood based raw material using at least an acid hydrolysis and a steam explosion to form a plant based feed, the wood based material including cellulose;
feeding the plant based feed to a first enzymatic hydrolysis stage, which is a continuous stage, and in which the plant based feed is hydrolysed, and the hydrolysis time of the first hydrolysis stage is below 24 hours;
separating a first liquid fraction comprising carbohydrates from a first solid fraction in the hydrolysed plant based feed in a first solid-liquid separation stage, wherein most of the enzymes are attached to the solid fraction;
recovering the first liquid fraction;
recirculating a first part of the first solid fraction comprising enzymes back to the plant based feed or to at least one of the enzymatic hydrolysis stages such that the feed of the first enzymatic hydrolysis stage after the recirculating comprises 20-60% by weight of the plant based feed and 40-80% by weight of the recirculated first part of the first solid fraction; and
feeding a second part of the first solid fraction to the next enzymatic hydrolysis stage, in which the second part of the first solid fraction is hydrolysed;
separating a second liquid fraction comprising carbohydrates from a second solid fraction in the hydrolysed second part of the first solid fraction in a second solid-liquid separation stage, wherein the second solid-liquid separation is carried out by a pressure filtration; and
recovering at least one of the second liquid fraction and the second solid fraction.

2. The method according to claim 1 , further comprising recirculating at least a portion of the second solid fraction to the plant based feed or to at least one of the enzymatic hydrolysis stages.

3. The method according to claim 1 , further comprising recirculating at least a portion of the first part of the first solid fraction to the plant based feed or to the same enzymatic hydrolysis stage.

4. The method according to claim 1 , wherein an average residence time of at least one of the enzymatic hydrolysis stages is 4-72 hours.

5. The method according to claim 1 , wherein the consistency of the plant based feed or the plant based raw material is 4-40 weight % in at least one of the enzymatic hydrolysis stages.

6. The method according to claim 1 , wherein the consistency of the plant based feed or the solid fraction is 10-40 weight % in the second or any later enzymatic hydrolysis stage.

7. The method according to claim 1 , wherein the plant based feed, plant based raw material, or solid fractions or its parts is diluted with liquid before the enzymatic hydrolysis.

8. The method according to claim 1 , wherein the liquid fractions are separated from the solid fractions using filtration, centrifugal treatment, or any combination thereof.

9. The method according to claim 1 , wherein at least one liquid fraction is recovered after the solid-liquid separation stages.

10. The method according to claim 1 , wherein an enzyme is added in connection with a first enzymatic hydrolysis stage or a second or later enzymatic hydrolysis stage or the enzymatic hydrolysis stage to which the first or second part of the first solid fraction is recirculated.

11. The method according to according to claim 1 , wherein the second or any later enzymatic hydrolysis stage is carried out without an enzyme addition.

12. The method according to claim 1 , wherein the first part of the solid fractions comprising enzymes are recirculated to the plant based feed.

Assignees

• Upm Kymmene Corp

Inventors

• Tamper Juha
• Turunen Sami

Classifications

• C13K1/02

  obtained by saccharification of cellulosic materials (manufacture of fodder A23K10/32) · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• Y02P20/582

  Recycling of unreacted starting or intermediate materials · CPC title

• C12P19/00 Primary

  Preparation of compounds containing saccharide radicals (ketoaldonic acids C12P7/58) · CPC title

• C12M21/18 Primary

  Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title