Method for producing polymer used as base film for cell culture, and cell culture container

Patent metadata

Field	Value
Publication number	US-12319765-B2
Application number	US-202318511083-A
Country	US
Kind code	B2
Filing date	Nov 16, 2023
Priority date	Aug 24, 2018
Publication date	Jun 3, 2025
Grant date	Jun 3, 2025

Abstract

The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I):wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

First claim

The invention claimed is:

1. A method for producing a polymer used as a base film for cell culture, which comprises
a preparation step of preparing a mixture containing
a monomer represented by the following formula (I):

wherein U a1 and U a2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, R a1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and R a2 represents a linear or branched alkylene group having 1 to 5 carbon atoms,
a monomer represented by the following formula (III):

wherein R c and R d each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, R e represents a linear or branched alkylene group having 1 to 5 carbon atoms, and n represents a number of 1 to 50,
a radical polymerization initiator and
an organic solvent, and
a polymerization step of preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer.

2. The method for producing the polymer according to claim 1 , wherein the mixture further contains a monomer represented by the formula (II):

wherein R b represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms.

3. The method for producing the polymer according to claim 2 , wherein a molar ratio of a unit derived from the monomer represented by the formula (I)/a unit derived from a monomer derived from the formula (II) in the polymer is 100/0 to 50/50.

4. The method for producing the polymer according to claim 1 , wherein a weight average molecular weight (Mn) of the polymer is 20,000 to 1,000,000, and a ratio (Mw/Mn) of the weight average molecular weight (Mw) and the number average molecular weight (Mn) of the polymer is 1.01 to 10.00.

5. The method for producing the polymer according to claim 1 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

6. The method for producing the polymer according to claim 2 , wherein a weight average molecular weight (Mn) of the polymer is 20,000 to 1,000,000, and a ratio (Mw/Mn) of the weight average molecular weight (Mw) and the number average molecular weight (Mn) of the polymer is 1.01 to 10.00.

7. The method for producing the polymer according to claim 3 , wherein a weight average molecular weight (Mn) of the polymer is 20,000 to 1,000,000, and a ratio (Mw/Mn) of the weight average molecular weight (Mw) and the number average molecular weight (Mn) of the polymer is 1.01 to 10.00.

8. The method for producing the polymer according to claim 2 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

9. The method for producing the polymer according to claim 3 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

10. The method for producing the polymer according to claim 4 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

11. A method for producing a polymer used as a base film for cell culture, which comprises
a preparation step of preparing a mixture containing
a monomer represented by the following formula (I):

wherein U a1 and U a2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, R a1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and R a2 represents a linear or branched alkylene group having 1 to 5 carbon atoms,
a radical polymerization initiator and
an organic solvent, and
a polymerization step of preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer,
wherein a weight average molecular weight (Mn) of the polymer is 20,000 to 1,000,000, and a ratio (Mw/Mn) of the weight average molecular weight (Mw) and the number average molecular weight (Mn) of the polymer is 1.01 to 10.00.

12. The method for producing the polymer according to claim 11 , wherein the mixture further contains a monomer having two or more carbon-carbon unsaturated bonds.

13. The method for producing the polymer according to claim 11 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

14. The method for producing the polymer according to claim 11 , wherein the mixture further contains a monomer represented by the formula (II):

wherein R b represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms.

15. The method for producing the polymer according to claim 14 , wherein the mixture further contains a monomer having two or more carbon-carbon unsaturated bonds.

16. The method for producing the polymer according to claim 14 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

17. The method for producing the polymer according to claim 14 , wherein a molar ratio of a unit derived from the monomer represented by the formula (I)/a unit derived from a monomer derived from the formula (II) in the polymer is 100/0 to 50/50.

18. The method for producing the polymer according to claim 17 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

19. The method for producing the polymer according to claim 17 , wherein the mixture further contains a monomer having two or more carbon-carbon unsaturated bonds.

20. The method for producing the polymer according to claim 19 , which is a polymer used as a base film for cell culture for obtaining cell aggregates by adhering cells and then detaching them.

Assignees

• Nissan Chemical Corp

Inventors

• Suzuki Kohei
• Hiroi Yoshiomi
• Abe Natsuki

Classifications

• C12N1/00

  Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• C08J5/18

  Manufacture of films or sheets · CPC title

• C08F220/06

  Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof · CPC title

• C08F2/44

  Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers · CPC title