Biconjugatable labels and methods of use

Patent metadata

Field	Value
Publication number	US-12312377-B2
Application number	US-201916510794-A
Country	US
Kind code	B2
Filing date	Jul 12, 2019
Priority date	Jul 13, 2018
Publication date	May 27, 2025
Grant date	May 27, 2025

Abstract

Aspects of the disclosure provide biconjugatable labels, labeled biomolecules, and methods of using and making the same.

First claim

What is claimed is:

1. A labeled biomolecule of the formula:

wherein:
Q 1 and Q 2 are independently monomeric or oligomeric biomolecules;
L 1 and L 2 are independently optionally substituted alkylene; and
X − is a counterion or is absent.

2. The labeled biomolecule of claim 1 , wherein Q 1 and Q 2 are independently nucleosides, nucleotides, oligonucleotides, nucleic acids, or derivatives or fragments thereof.

3. The labeled biomolecule of claim 2 , wherein Q 1 and/or Q 2 form a first oligonucleotide strand.

4. The labeled biomolecule of claim 3 , further comprising a second oligonucleotide strand hybridized to the first oligonucleotide strand.

5. The labeled biomolecule of claim 1 , wherein a nucleoside polyphosphate is attached through a terminal phosphate to Q 1 and/or Q 2 .

6. The labeled biomolecule of claim 1 , wherein the labeled biomolecule is of the formula:

wherein n is independently an integer from 1-20, inclusive.

7. The labeled biomolecule of claim 6 , wherein the labeled biomolecule is of the formula:

8. The labeled biomolecule of claim 7 , wherein the labeled biomolecule is of the formula:

9. The labeled biomolecule of claim 1 , wherein L 1 and L 2 are independently optionally substituted C 1-20 alkylene.

10. The labeled biomolecule of claim 9 , wherein L 1 and L 2 are independently optionally substituted C 1-10 alkylene.

11. The labeled biomolecule of claim 3 , wherein the first oligonucleotide strand comprises at least 10 and fewer than 200 nucleotides.

12. The labeled biomolecule of claim 11 , wherein the first oligonucleotide strand comprises at least 10 and fewer than 100 nucleotides.

13. The labeled biomolecule of claim 12 , wherein the first oligonucleotide strand comprises at least 10 and fewer than 50 nucleotides.

14. The labeled biomolecule of claim 1 , wherein Q 1 and Q 2 are independently oligonucleotides, nucleic acids, or derivatives or fragments thereof, and wherein Q 1 and Q 2 form a first oligonucleotide strand.

15. The labeled biomolecule of claim 14 , further comprising a second oligonucleotide strand hybridized to the first oligonucleotide strand.

Assignees

• Quantum Si Inc

Inventors

• Rothberg Jonathan M
• Lackey Jeremy
• Nani Roger
• Dodd David

Classifications

• C09B57/00

  Other synthetic dyes of known constitution · CPC title

• C09B23/0075

  the polymethine chain being part of an heterocyclic ring · CPC title

• C09B11/24

  Phthaleins containing amino groups {; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes} · CPC title

• C07F5/022

  without C-boron linkages · CPC title

• A61K49/0032

  Methine dyes, e.g. cyanine dyes · CPC title