Biomarkers and immunogenic compositions for filarial parasites

Patent metadata

Field	Value
Publication number	US-12311016-B2
Application number	US-202318173288-A
Country	US
Kind code	B2
Filing date	Feb 23, 2023
Priority date	Apr 1, 2016
Publication date	May 27, 2025
Grant date	May 27, 2025

Abstract

Disclosed herein are immunogenic compositions for preventing or treating infection with filarial parasites and biomarkers for diagnosing infection with filarial parasites.

First claim

What is claimed is:

1. A method for determining whether a subject has been infected with Onchocerca volvulus , comprising identifying in a specimen from the subject antibodies to O. volvulus filarial proteins Ov16 (SEQ ID NO:63) and OVOC3261 (SEQ ID NO:4), or an immunoreactive fragment thereof, and optionally one or more of OVOC10469 (SEQ ID NO:1), OVOC11950 (SEQ ID NO:2), OVOC10602 (SEQ ID NO:3), OVOC5127 (SEQ ID NO:5), OVOC8491 (SEQ ID NO:6), OVOC6759 (SEQ ID NO:7), OVOC451 (SEQ ID NO:8), OVOC12329 (SEQ ID NO:9), OVOC3337 (SEQ ID NO:10), OVOC10264 (SEQ ID NO:11), OVOC4230 (SEQ ID NO:12), OVOC10384 (SEQ ID NO:13), OVOC8422 (SEQ ID NO:14), OVOC9988 (SEQ ID NO:31), and OVOC6395 (SEQ ID NO:15), or an immunoreactive fragment thereof, wherein presence of antibodies to at least Ov16 and OVOC3261 in the specimen from the subject indicates that the subject has been infected with O. volvulus.

2. The method of claim 1 , wherein the specimen comprises blood or urine.

3. The method of claim 1 , wherein the immunoreactive fragment comprises the amino acid sequence of OVOC10469_Pep2 (SEQ ID NO:51), OVOC3261_Pep1 (SEQ ID NO:52), OVOC3261_Pep3 (SEQ ID NO:53), OVOC10469_Pep1 (SEQ ID NO:54), OVOC10469_Pep3 (SEQ ID NO:55), OVOC3261_Pep2 (SEQ ID NO:56), OVOC5127_Pep1 (SEQ ID NO:57), OVOC5127_Pep2 (SEQ ID NO:58), OVOC5127_Pep4, (SEQ ID NO:59), OVOC5127_Pep5 (SEQ ID NO:60), or OVOC5127_PepX (SEQ ID NO:61).

4. The method of claim 1 , comprising identifying in the specimen from the subject antibodies to O. volvulus filarial proteins Ov16 and OVOC3261, or an immunogenic fragment thereof, and one or more O. volvulus filarial protein selected from OVOC10469, OVOC5127, OVOC9988, and OVOC4230, or an immunogenic fragment thereof.

5. The method of claim 1 , comprising identifying in the specimen from the subject antibodies to O. volvulus filarial proteins Ov16, OVOC3261, OVOC10469, and OVOC5127, or an immunogenic fragment thereof.

6. The method of claim 1 , wherein the antibodies to the O. volvulus filarial proteins are detected by a method selected from the group consisting of ELISA, dipstick tests, lateral flow, microfluidic devices, luciferase immunoprecipitation systems, luminex, multiplex-formats, and microarrays.

Assignees

• New York Blood Center Inc
• Us Health

Inventors

• Lustigman Sara
• Nutman Thomas B
• Bennuru Sasisekhar

Classifications

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• A61K2039/552

  Veterinary vaccine · CPC title

• A61K2039/6031

  Proteins · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title

• A61K2039/55505

  Inorganic adjuvants · CPC title