Human tissue derived compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-12310993-B2
Application number	US-201816229823-A
Country	US
Kind code	B2
Filing date	Dec 21, 2018
Priority date	Jun 24, 2016
Publication date	May 27, 2025
Grant date	May 27, 2025

Abstract

Disclosed are compositions comprising a non-homogenized chorionic matrix, a homogenized amniotic matrix and a homogenized UC (UC) matrix, wherein the non-homogenized chorionic matrix comprises viable cells. Disclosed are methods of making the compositions disclosed herein comprising preparing a non-homogenized chorionic matrix, preparing a homogenized amniotic matrix, preparing a homogenized UC matrix, and combining the non-homogenized chorionic matrix, the non-homogenized chorionic matrix, and the homogenized UC matrix. Disclosed are methods of treating a tissue injury or chronic pain comprising administering any of the disclosed compositions to an area of a subject comprising a tissue injury.

First claim

We claim:

1. A composition comprising a minced chorionic matrix and a devitalized homogenized umbilical cord (UC) matrix,
wherein the minced chorionic matrix comprises native cells,
wherein the composition does not comprise trophoblasts or an amniotic matrix,
wherein the composition is lyophilized,
wherein at least 70% of the native cells of the minced chorionic matrix are viable after storage at room temperature in the lyophilized state,
wherein the minced chorionic matrix and the devitalized homogenized UC matrix are each in particulate form,
wherein the minced chorionic matrix has a larger average particle size than the devitalized homogenized UC matrix, and
wherein the devitalized homogenized UC matrix does not include Wharton's jelly.

2. The composition of claim 1 , wherein the composition is formulated as a cream, gel, oil, ointment, or lotion.

3. The composition of claim 1 further comprising viable, isolated amniotic cells.

4. The composition of claim 1 , wherein the homogenized UC matrix is not decellularized.

5. The composition of claim 1 , wherein the minced chorionic matrix is not homogenized.

6. The composition of claim 1 , wherein the composition comprises viable chorionic stem cells, amniotic stem cells, fibroblasts, epithelial cells or a combination thereof.

7. The composition of claim 1 , wherein the minced chorionic matrix and homogenized UC matrix are from the same donor.

8. The composition of claim 1 , wherein the homogenized UC matrix comprises de-veined UC tissue.

9. The composition of claim 1 , wherein the minced chorionic matrix comprises greater than or equal to 100,000 viable cells/ml.

10. The composition of claim 1 , wherein at least 75% of the native cells of the minced chorionic matrix are viable after storage at room temperature in the lyophilized state.

11. The composition of claim 1 , wherein the composition further comprises a pharmaceutically acceptable excipient.

12. The composition of claim 1 , wherein the homogenized UC matrix is 10 nm to 1 mm in size.

13. A pharmaceutical composition comprising the composition of claim 1 and a pharmaceutically acceptable carrier.

Assignees

• Osiris Therapeutics Inc

Inventors

• Sinclair Steven Michael
• Danilkovitch Alla
• Sathyamoorthy Malathi
• Kuang Jin-Qiang
• Dhall Sandeep
• Liu Yishan
• Melchiorri Anthony John
• Moorman Matthew Robert
• Schiano Lo Moriello Mena
• Allgood Lerch Anne

Classifications

• A61K35/51

  Umbilical cord; Umbilical cord blood; Umbilical stem cells · CPC title

• A61L26/0057

  Ingredients of undetermined constitution or reaction products thereof · CPC title

• A61L27/3834

  Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells · CPC title

• A61L27/3604

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61P19/02

  for joint disorders, e.g. arthritis, arthrosis · CPC title