Composition for diagnosis of degenerative neurological diseases

Patent metadata

Field	Value
Publication number	US-12306191-B2
Application number	US-201916982500-A
Country	US
Kind code	B2
Filing date	Mar 21, 2019
Priority date	Mar 21, 2018
Publication date	May 20, 2025
Grant date	May 20, 2025

Abstract

The present invention relates to a composition for diagnosis of degenerative neurological diseases and, more specifically, to a composition for diagnosis of degenerative neurological diseases, comprising an agent for measuring the level of acetylation of COX2.
According to the present invention, since acetylation of COX2 in degenerative neurological diseases is significantly reduced, whether COX2 is acetylated may be utilized as a diagnostic marker for degenerative neurological diseases, and it is possible to diagnose degenerative neurological diseases more rapidly and accurately by using same.

First claim

What is claimed is:

1. A method of treatment, comprising:
(a) providing a biological sample from a patient;
(b) measuring the level of acetylation of COX2 (cyclooxygenase-2) in the sample;
(c) identifying the sample as having a level of acetylation of the COX2 that is lower than that of a normal control sample; and
(d) treating the patient for a degenerative neurological disease using a COX2 acetylating agent based on the results of step (c).

2. The method of claim 1 , wherein the method for measuring the level of acetylation of COX2 is at least one selected from the group consisting of autoradiography, liquid scintillation counting, molecular weight assay, liquid chromatographic mass assay, Western blot, ELISA, radioimmunoassay, radioimmunodiffusion, Ouchterlony immunodiffusion, rocket immunoelectrophoresis, immunofluorescence staining, immunohistochemical staining, immunoprecipitation assay, complement fixation assay, FACS, and protein chip.

3. The method of claim 1 , wherein the sample is selected from the group consisting of blood, blood cells, brain tissue, nerve cells, cerebrospinal fluid, saliva, nasal fluid, sputum, synovial fluid, amniotic fluid, ascites, cervical or vaginal secretions, and urine.

Assignees

• Kyungpook National Univ Industry—Academic Cooperation Foundation
• Kyungpook Nat Univ Ind Academic Coop Found

Inventors

• Bae Jae-Sung
• Jin Hee Kyung
• Lee Ju Youn
• Han Seung Hoon

Classifications

• G01N2333/90241

  acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title

• G01N33/573

  for enzymes or isoenzymes · CPC title

• G01N2440/10

  acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation · CPC title

• G01N33/5008

  for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics · CPC title

• A61K31/164 Primary

  of a carboxylic acid with an aminoalcohol, e.g. ceramides · CPC title