Method for detecting indicator of immune-related disease

Patent metadata

Field	Value
Publication number	US-12306171-B2
Application number	US-201916977918-A
Country	US
Kind code	B2
Filing date	Mar 8, 2019
Priority date	Mar 9, 2018
Publication date	May 20, 2025
Grant date	May 20, 2025

Abstract

Provided is a method whereby an objective indicator of an immune-related disease can be rapidly and easily detected, the method comprising detecting, as an indicator of an immune-related disease, a difference in the result of observing a primary cilium of an immune-related cell between a subject specimen and a normal specimen.

First claim

The invention claimed is:

1. A method for detecting an index of an immune-related disease in a subject specimen collected from a subject, comprising the steps of:
observing primary cilia of immune-related cells in the subject specimen and primary cilia of immune-related cells in a normal specimen,
counting a number of immune-related cells having the primary cilia or measuring a length of the primary cilia,
comparing the number of immune-related cells having the primary cilia or the length of the primary cilia of the immune-related cells in the subject specimen with the number of immune-related cells having the primary cilia or the length of the primary cilia of the immune-related cells in the normal specimen,
detecting a difference in the number of immune-related cells having the primary cilia or the length of the primary cilia between the primary cilia of the immune-related cells in the subject specimen and the primary cilia of the immune-related cells in the normal specimen, and
using the difference as an index of the immune-related disease,
wherein the immune-related disease is atopic dermatitis or asthma, and the immune-related cells are Langerhans cells, keratinocytes, or peripheral blood mononuclear cells, and
wherein the index is a formation rate of primary cilia of immune-related cells in the subject specimen being either higher or lower than a formation rate of primary cilia of immune-related cells in the normal specimen.

2. A method for assisting diagnosis of an immune-related disease in a subject, comprising the steps of:
observing primary cilia of immune-related cells in a subject specimen collected from the subject and primary cilia of immune-related cells in a normal specimen to be compared,
counting a number of immune-related cells having the primary cilia or measuring a length of the primary cilia,
comparing the number of immune-related cells having the primary cilia or the length of the primary cilia of the immune-related cells in the subject specimen with the number of immune-related cells having the primary cilia or the length of the primary cilia of the immune-related cells in the normal specimen to be compared, and
obtaining a comparison result indicating that the number of immune-related cells each having the primary cilia in the subject specimen is larger than the number of immune-related cells each having primary cilia in the normal specimen to be compared, a comparison result indicating that the formation rate of the primary cilia of the immune-related cells in the subject specimen is higher than the formation rate of the primary cilia of the immune-related cells in the normal specimen to be compared, or a comparison result indicating that the length of the primary cilia of the immune-related cells in the subject specimen is longer than the length of the primary cilia of the immune-related cells in the normal specimen to be compared, and
providing the comparison result to a doctor for a diagnosis by the doctor of the presence or absence of infection with the immune-related disease in the subject or information for assisting diagnosis or prognosis of the immune-related disease in the subject, on the basis of the resulting comparison result,
wherein the immune-related disease is atopic dermatitis or asthma, and the immune-related cells are Langerhans cells, keratinocytes, or peripheral blood mononuclear cells, and
wherein the index is a formation rate of primary cilia of immune-related cells in the subject specimen being either higher or lower than a formation rate of primary cilia of immune-related cells in the normal specimen.

3. The method for detecting an index of an immune-related disease in a subject of claim 1 , wherein the immune-related disease is atopic dermatitis.

4. The method for detecting an index of an immune-related disease in a subject of claim 1 , wherein the immune-related disease is asthma.

5. The method for assisting diagnosis of an immune-related disease in a subject of claim 2 , wherein the immune-related disease is atopic dermatitis.

6. The method for assisting diagnosis of an immune-related disease in a subject of claim 2 , wherein the immune-related disease is asthma.

7. The method for detecting an index of an immune-related disease in a subject of claim 1 , wherein the immune-related cells are Langerhans cells.

8. The method for detecting an index of an immune-related disease in a subject of claim 1 , wherein the immune-related cells are keratinocytes.

9. The method for detecting an index of an immune-related disease in a subject of claim 1 , wherein the immune-related cells are peripheral blood mononuclear cells.

Assignees

• Mandom Corp
• Univ Osaka
• Nat Inst Biomedical Innovation Health & Nutrition

Inventors

• TORIYAMA Manami
• Fujita Fumitaka
• Okada Fumihiro
• Ishii Ken

Classifications

• G01N2800/52

  Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis · CPC title

• G01N2800/24

  Immunology or allergic disorders · CPC title

• G01N33/56966

  Animal cells · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title