Compositions and Methods for Enhancing the Stability of Transgenes in Poxviruses
US-2020061174-A1 · Feb 27, 2020 · US
Method for producing artificial recombinant RNA virus stably retaining foreign gene
US12305192B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12305192-B2 |
| Application number | US-202017619899-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 19, 2020 |
| Priority date | Jun 21, 2019 |
| Publication date | May 20, 2025 |
| Grant date | May 20, 2025 |
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- Title
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Abstract
Official abstract text for this publication.
Provided is a method for producing an artificial recombinant RNA virus stably expressing a foreign gene, comprising the steps of:(1) obtaining a foreign gene having a modified codon composition similar to that of an RNA virus gene;(2) inserting the foreign gene obtained in step (1) into an RNA virus genome; and(3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing an artificial recombinant RNA virus stably retaining a foreign gene, comprising the steps of: (1) obtaining a foreign gene having a codon composition modified to such an extent that the difference in the codon composition between the foreign gene and an RNA virus gene is within +30%; (2) inserting the foreign gene obtained in step ( 1 ) into an RNA virus genome; and (3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics, wherein the RNA virus is a virus belonging to the family Reoviridae. 2. The method according to claim 1 , wherein the virus belonging to the family Reoviridae is a virus belonging to the genus Rotavirus or Orthoreovirus. 3. A method for enabling stable retention of an introduced foreign gene in an artificial recombinant RNA virus artificially synthesized using reverse genetics, comprising modifying the codon composition of a foreign gene to such an extent that the difference in the codon composition between the foreign gene and an RNA virus gene is within +30%, wherein the RNA virus is a virus belonging to the family Reoviridae. 4. The method according to claim 3 , wherein the virus belonging to the family Reoviridae is a virus belonging to the genus Rotavirus or Orthoreovirus.
Assignees
Inventors
Classifications
viral genome or elements thereof as genetic vector · CPC title
New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title
Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title
Vectors comprising a coding region that has been codon optimised for expression in a respective host · CPC title
- C12N15/867Primary
Retroviral vectors · CPC title
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- What does patent US12305192B2 cover?
- Provided is a method for producing an artificial recombinant RNA virus stably expressing a foreign gene, comprising the steps of:(1) obtaining a foreign gene having a modified codon composition similar to that of an RNA virus gene;(2) inserting the foreign gene obtained in step (1) into an RNA virus genome; and(3) artificially synthesizing an artificial recombinant RNA virus using reverse genet…
- Who is the assignee on this patent?
- Univ Osaka
- What technology area does this patent fall under?
- Primary CPC classification C12N15/867. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 20 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).