Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-12305178-B2
Application number	US-202218050791-A
Country	US
Kind code	B2
Filing date	Oct 28, 2022
Priority date	Mar 14, 2013
Publication date	May 20, 2025
Grant date	May 20, 2025

Abstract

Recombinant DNA molecules and constructs including sequences derived from Digitaria sanguinalis are provided, which are useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds are also provided, comprising a recombinant DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of their use.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 95 percent sequence identity to the full length of SEQ ID NO:7, wherein the sequence has promoter activity;
b) a DNA sequence comprising SEQ ID NO: 7; and
c) a fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 7, wherein the fragment has promoter activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

2. The recombinant DNA molecule of claim 1 , wherein said DNA sequence has at least 90-97 percent sequence identity to the DNA sequence of SEQ ID NO: 7, wherein the sequence has promoter activity.

3. The recombinant DNA molecule of claim 1 , wherein said DNA sequence has at least 99 percent sequence identity to the DNA sequence of SEQ ID NO:7, wherein the sequence has promoter activity.

4. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

5. The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

6. The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers pest resistance in plants.

7. A transgenic plant cell comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 85-95 percent sequence identity to the full length of SEQ ID NO: 7, wherein the sequence has promoter activity;
b) a DNA sequence comprising SEQ ID NO: 7; and
c) a fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 7, wherein the fragment has promoter activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

8. The transgenic plant cell of claim 7 , wherein said transgenic plant cell is a monocotyledonous plant cell.

9. The transgenic plant cell of claim 7 , wherein said transgenic plant cell is a dicotyledonous plant cell.

10. A transgenic plant, or part thereof, comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 95 percent sequence identity to the full length of SEQ ID NO: 7, wherein the sequence has promoter activity;
b) a DNA sequence comprising SEQ ID NO: 7; and
c) a fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 7, wherein the fragment has promoter activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

11. A progeny plant of the transgenic plant of claim 10 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

12. A transgenic seed of the transgenic plant of claim 10 , wherein the seed comprises the recombinant DNA molecule.

13. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 10 and producing the commodity product therefrom.

14. The method of claim 13 , wherein the commodity product is processed seeds, grains, plant parts, and meal.

15. A method of producing a transgenic plant comprising:
a) transforming a plant cell with the recombinant DNA molecule of claim 1 to produce a transformed plant cell; and
b) regenerating a transgenic plant from the transformed plant cell.

16. The recombinant DNA molecule of claim 1 , wherein the DNA sequence has at least 95 percent sequence identity to the full length of SEQ ID NO: 7, and wherein the sequence has promoter activity.

17. The recombinant DNA molecule of claim 1 , wherein the DNA sequence comprises SEQ ID NO: 7.

18. The recombinant DNA molecule of claim 1 , wherein the DNA sequence comprises the fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 7, and wherein the fragment has promoter activity.

Assignees

• Monsanto Technology Llc

Inventors

• Flasinski Stanislaw
• Zhang Jun
• Zhao Suling

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12N15/8227

  Root-specific · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title