Resonance Raman spectroscopy for evaluating mitochondrial function

Patent metadata

Field	Value
Publication number	US-12298247-B2
Application number	US-202017772085-A
Country	US
Kind code	B2
Filing date	Oct 27, 2020
Priority date	Oct 28, 2019
Publication date	May 13, 2025
Grant date	May 13, 2025

Abstract

Apparatuses and methods for using Raman Resonance Spectroscopy to evaluate metabolic and oxygenation status of the eye are disclosed herein. In some embodiments, metabolic mapping of the eye may be performed by aligning a Raman spectrum and a recorded spatial image of the eye.

First claim

What is claimed is:

1. A device comprising:
a Raman spectrometer arranged to collect inelastic light;
a Raman probe in communication with the Raman spectrometer, the Raman probe arranged to transmit light to a portion of a subject and collect the inelastic light back to the spectrometer; and
a first imager arranged to capture a spatial image of the portion of the subject,
wherein the device is configured to generate a Raman spectrum from the collected inelastic light, and wherein the device is configured to calculate an oxidized ratio, a reduced ratio, and a total quantity of a first molecule using the Raman spectrum.

2. The device of claim 1 , further comprising one or more processors configured to generate the Raman spectrum from the collected inelastic light, and wherein the one or more processors are configured to align the Raman spectrum with the spatial image of the portion of the subject.

3. The device of claim 1 , further comprising an objective lens, the objective lens being in communication with each of the Raman probe and the first imager.

4. The device of claim 1 , further comprising a scanning mirror arranged to adjust a scanning area of the Raman probe.

5. The device of claim 1 , further comprising an excitation source arranged to supply an excitation laser to the Raman probe.

6. The device claim 1 , further comprising one or more beam splitters.

7. The device of claim 1 , wherein the first imager includes one of a fundus camera and an anterior segment camera.

8. The device of claim 1 , wherein the Raman spectrometer includes a filter and a charge coupled device detector.

9. The device of claim 1 , further comprising one or more processers configured to generate the Raman spectrum from the collected inelastic light, and wherein the one or more processors are configured to calculate the oxidized ratio, the reduced ratio, and the total quantity of the first molecule using the Raman spectrum.

10. The device of claim 1 , wherein the light transmitted by the Raman probe has a wavelength of one of 441 nm and 430 nm.

11. The device of claim 1 , wherein the device is configured to align the Raman spectrum with the spatial image of the portion of the subject to associate a histological change of the subject with the Raman spectrum.

Assignees

• Childrens Medical Center
• Pendar Tech Llc

Inventors

• Smith Lois
• Cakir Bertan D
• Romfh John Padraic
• Chen Peili
• Vakhshoori Daryoosh

Classifications

• G01J3/44

  Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} · CPC title

• A61B3/12

  for looking at the eye fundus, e.g. ophthalmoscopes (A61B3/13 takes precedence) · CPC title

• A61B3/102

  for optical coherence tomography [OCT] · CPC title

• G01N21/65 Primary

  Raman scattering · CPC title

• A61B3/10 Primary

  Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions · CPC title