Method for determining and optimizing the content of at least one plant substance of at least one part of a plant

Patent metadata

Field	Value
Publication number	US-12298246-B2
Application number	US-202017778532-A
Country	US
Kind code	B2
Filing date	Nov 20, 2020
Priority date	Nov 22, 2019
Publication date	May 13, 2025
Grant date	May 13, 2025

Abstract

Described and represented is a method for determining the content of at least one plant substance of at least one part of a plant. In order for the content of plant substances, in particular secondary plant substances, of at least one part of a plant to be determined and optimized more expediently, it is provided that the at least one part of the plant is irradiated successively with light of different wavelengths and/or wavelength ranges and that, in response to the irradiation of the at least one part of the plant with light of each wavelength and/or at each wavelength range, the chlorophyll fluorescence of at least substantially the same wavelength and/or at least substantially the same wavelength range is measured in each case.

First claim

The invention claimed is:

1. A method for determining the content of at least one plant substance of at least one part of a plant, comprising:
successively irradiating the at least one part of the plant with light of different wavelengths and/or wavelength ranges and
in response to irradiating the at least one part of the plant with light of each wavelength and/or at each wavelength range, measuring the chlorophyll fluorescence of at least the same wavelength and/or at least the same wavelength range in each case.

2. The method according to claim 1 , further comprising:
comparing the measured values of the chlorophyll fluorescence with one another and/or with reference values.

3. The method according to claim 1 , further comprising:
successively irradiating the at least one part of the plant with light of at least three different wavelengths and/or wavelength ranges and
in response to irradiating the at least one part of the plant with light of the at least three different wavelengths and/or wavelength ranges, measuring the chlorophyll fluorescence of at least the same wavelength and/or at least the same wavelength range in each case.

4. The method according to claim 1 , further comprising:
selecting at least one of the different wavelengths and/or wavelength ranges at least in the range of an absorption maxima of at least one plant substance.

5. The method according to claim 1 , further comprising:
selecting at least one of the different wavelengths and/or wavelength ranges at least in the range of an absorption maxima of a chlorophyll.

6. The method according to claim 1 , further comprising:
from the measured values of the chlorophyll fluorescence, recording a response function as a function of the wavelengths and/or wavelength ranges used for irradiating the at least one part of the plant.

7. The method according to claim 1 , further comprising:
in response to irradiating the at least one part of the plant with light of each wavelength and/or at each wavelength range, measuring the chlorophyll fluorescence of at least the same wavelength and/or at least the same wavelength range in each case at different locations of the at least one part of the plant.

8. The method according to claim 7 , further comprising:
separately comparing the measured values of the chlorophyll fluorescence for each location with one another and/or with reference values and/or
from the measured values of the chlorophyll fluorescence for each location, separately recording response functions as a function of the wavelengths and/or wavelength ranges used for irradiating the at least one part of the plant.

9. The method according to claim 1 , further comprising:
determining the at least one plant substance of at least one part of a leaf of a plant, of a leaf of a plant, of a plurality of leaves of a plant, of all leaves of a plant, of an entire plant, of at least parts of a plurality of plants or of a plurality of plants in total,
irradiating at least one part of a leaf of a plant, a leaf of a plant, a plurality of leaves of a plant, all leaves of a plant, an entire plant, at least parts of a plurality of plants or a plurality of plants with light of different wavelengths and/or wavelength ranges, and
measuring the chlorophyll fluorescence of a certain wavelength and/or of a certain wavelength range of the at least one part of a leaf of a plant, of a leaf of a plant, of a plurality of leaves of a plant, of all leaves of a plant, of an entire plant, of at least parts of a plurality of plants or of a plurality of plants.

10. The method according to claim 1 , further comprising:
determining the values of the chlorophyll fluorescence from grey-scale values of images and/or pixels recorded by means of a camera.

11. The method according to claim 1 , further comprising:
determining the at least one plant substance of at least one part of a plant in-vivo and/or
determining a concentration of the at least one plant substance.

12. The method according to claim 1 , further comprising:
successively irradiating the at least one part of the plant with pulsed light of different wavelengths and/or wavelength ranges and/or
measuring the chlorophyll fluorescence in transmission and/or reflection in relation to the irradiation of the at least one part of the plant.

13. A method for optimizing the content of at least one plant substance of at least one plant at the time of harvesting the at least one plant, comprising:
determining the content of the at least one plant substance of at least one part of the at least one plant using the method according to claim 1 ; and
selecting the time of harvest according to the determined content of the at least one plant substance of the at least one part of the at least one plant.

14. A method for optimizing the content of at least one plant substance of at least one plant at the time of harvesting the at least one plant, comprising:
determining the content of the at least one plant substance of at least one part of the at least one plant using the method according to claim 1 ; and
controlling at least one growth condition of the at least one plant according to predefined criteria on the basis of the determined content of the at least one plant substance of the plant.

15. A method for optimizing the content of at least one plant substance of at least one plant at the time of harvesting the at least one plant, comprising:
determining the content of the at least one plant substance of at least one part of the at least one plant using the method according to claim 1 ; and
regulating at least one growth condition of the at least one plant according to predefined criteria on the basis of the determined content of the at least one plant substance of the plant.

Assignees

• Fraunhofer Ges Forschung

Inventors

• Keuter Volkmar
• Schlehuber Dennis
• Somborn Annette
• Wack Holger
• Deckert Stephan
• Katayama Victor Takazi
• Thoma Felix

Classifications

• G01N2201/0627

  Use of several LED's for spectral resolution · CPC title

• G01N2021/635

  Photosynthetic material analysis, e.g. chrorophyll · CPC title

• G01N2201/06193

  Secondary in situ sources, e.g. fluorescent particles · CPC title

• G01N21/6486 Primary

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• A01G7/00 Primary

  Botany in general · CPC title