Single cell encapsulation via pickering emulsion for bio-pesticides application

Patent metadata

Field	Value
Publication number	US-12290068-B2
Application number	US-201917059233-A
Country	US
Kind code	B2
Filing date	May 27, 2019
Priority date	May 27, 2018
Publication date	May 6, 2025
Grant date	May 6, 2025

Abstract

This invention provides a systems and methods for single cell microencapsulation by eco-friendly oil-in-water (o/w) Pickering emulsion.

First claim

The invention claimed is:

1. A method for single cell encapsulation comprising:
providing a water-based solution including amine functionalized silica nanoparticles;
emulsifying the water-based solution by adding an oil solution into the water-based solution to form oil-in-water Pickering emulsions;
adding cells of Metarhizium spore into said solution containing the oil-in-water Pickering emulsions;
generating at least one single cell encapsulation of the cells by vortexing, wherein the content of the nanoparticles is between 0.1 wt % to 10 wt %, and the oil/water ratio is one of: 1:99, 5:95, 10:90, 20:80, 30:70, 40:60 and 50:50 respectively.

2. The method of claim 1 , wherein said oil-based solution is one of: paraffin oil, silicone oil, sunflower oil, and mineral oil.

3. The method of claim 1 , wherein said Metarhizium is one of Metarhizium anisopliae, Metarhizium brunneum, Metarhizium robertsii, Metarhizium frigidum, Metarhizium riley, Metarhizium acridum.

4. The method of claim 3 wherein said Metarhizium brunneum is Metarhizium brunneum Mb7.

5. The method of claim 1 , wherein said nanoparticles are functionalized with silane selected from the group consisting of: Acryloxymethyltrimethoxysilane, (3-acryloxypropyl)trimethoxysilane, 3-aminopropyltrimethoxysilane, 3-aminopropyltriethoxysilane, n-(2-aminoethyl)-3-aminopropyltrimethoxysilane, Ethyltrimethoxysilane, n-propyltriethoxysilane, Isobutyltriethoxysilane, Hexyltrimethoxysilane, Octyltrimethoxysilane, 4-Aminobutyltriethoxysilane, 3-Aminopropyldiisopropylethoxysilane, 3-Mercaptopropyltriethoxysilane, 3-Mercaptopropyltrimethoxysilane, 1-Mercaptoundecyltrimethoxysilane, S-(Octanoyl)Mercaptopropyltriethoxysilane, 1H,1H,2H,2H-Perfluorooctyltrimethoxysilane, (3,3,3-Trifluoropropyl) trimethoxysilane, n-Octylmethyldiethoxysilane, (3,3,3-trifluoropropyl) trimethoxysilane, Undecyltrichlorosilane, Dodecyltriethoxysilane, Docosyltriethoxysilane, Dimethyldimethoxysilane, Dimethyldichlorosilane, n-Butyltriethoxysilane, (3-glycidyloxypropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyltrimethoxysilane, 3-isocyanatopropyltriethoxysilane, bis[3-(triethoxysilyl)propyl]tetrasulfide.

6. A method for pest control comprising:
(i) generating the emulsion comprising encapsulated single cells according to the method defined in any one of the preceding claims and;
(ii) applying the emulsion onto at least one leaf.

7. The method of claim 6 , wherein said at least one leaf is a R. communis Leaf.

Assignees

• The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro
• The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization

Inventors

• Mechrez Guy
• Ment Dana

Classifications

• C12N11/14

  Enzymes or microbial cells immobilised on or in an inorganic carrier · CPC title

• C12N11/04

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• C12N5/0012

  Cell encapsulation · CPC title

• A01N25/28 Primary

  Microcapsules {or nanocapsules} · CPC title