Sample analysis with mirrors

Patent metadata

Field	Value
Publication number	US-12287350-B2
Application number	US-201917056142-A
Country	US
Kind code	B2
Filing date	May 17, 2019
Priority date	May 18, 2018
Publication date	Apr 29, 2025
Grant date	Apr 29, 2025

Abstract

A device is disclosed, including: a plate including a sample deposition tube adapted to receive a sample comprising one or more analytes, the sample deposition tube defining a vertical axis substantially perpendicular to a main surface of the plate; and a mirror positioned to project, substantially parallel to the main surface of the plate, an image of the sample deposition tube along its vertical axis, so as to allow determination of the vertical location of the one or more analytes inside the sample deposition tube. Methods for determining the density of one or more analytes using various embodiments of the device are also described.

First claim

The invention claimed is:

1. A device, comprising:
a plate comprising a sample deposition tube adapted to receive a sample comprising one or more analytes, the sample deposition tube defining a vertical axis substantially perpendicular to a main surface of the plate; and
a mirror positioned to project, substantially parallel to the main surface of the plate, an image of the sample deposition tube along its vertical axis, so as to allow determination of the vertical location of the one or more analytes inside the sample deposition tube.

2. The device of claim 1 , wherein the mirror is positioned at about 40° to about 50° with respect to the vertical axis substantially perpendicular to the main surface of the plate.

3. The device of claim 2 , wherein the mirror is positioned at about 45° with respect to the vertical axis substantially perpendicular to the main surface of the plate.

4. The device of claim 1 , further comprising a plurality of said sample deposition tubes and one or more said mirrors, wherein each one of the plurality of mirrors is positioned to project, substantially parallel to the main surface of the plate, an image of one or more of the plurality of the sample deposition tubes along the vertical axis substantially perpendicular to the main surface of the plate.

5. The device of claim 1 , further comprising an image-capturing device.

6. The device of claim 5 , wherein the image-capturing device comprises a flatbed scanner, a camera, or a combination thereof.

7. The device of claim 1 , further comprising one or more relay lenses configured to focus the projected image.

8. The device of claim 1 , wherein the main surface of the plate is defined by an x-axis and a y-axis, and wherein the plate comprises m sample deposition tubes arranged in a row along the x-axis, n sample deposition tubes arranged in a row along the y-axis, and a plurality of said mirrors each positioned to project, substantially parallel to the main surface of the plate, an image of one of the sample deposition tubes along its vertical axis.

9. The device of claim 8 , wherein m and n are 2 and 3, 3 and 4, 4 and 6, 6 and 8, 8 and 12, 16 and 24, or 32 and 48, respectively.

10. The device of claim 1 , wherein the plate comprises 6, 12, 24, 48, 96, 384, or 1536 said sample deposition tubes.

11. The device of claim 10 , wherein the plate comprises 96 said sample deposition tubes.

12. The device of claim 1 , further comprising a first magnet and a second magnet disposed adjacent to the sample deposition tube to impart a magnetic field that is inside the sample deposition tube; wherein each magnet comprises a first pole and a second pole, and the first pole of the first magnet faces the first pole of the second magnet.

13. The device of claim 12 , further comprising a third magnet disposed underneath the first magnet and a fourth magnet disposed underneath the second magnet; wherein each of the third and fourth magnets comprises a first pole and a second pole, and the second pole of the third magnet faces the second pole of the second magnet.

14. The device of claim 12 , further comprising a plurality of said sample deposition tubes and a plurality of said first and second magnets.

15. The device of claim 1 , wherein the sample comprises a paramagnetic medium.

16. The device of claim 15 , wherein the paramagnetic medium comprises a paramagnetic salt, a paramagnetic hydrophobic metal chelate, or a paramagnetic ionic liquid.

17. The device of claim 16 , wherein the paramagnetic salt comprises MnCl 2 , GdCl 3 , DyCl 3 , MnBr 2 , CuSO 4 , FeCl 2 , or HoCl 3 .

18. The device of claim 16 , wherein the metal chelate comprises gadopentetate dimeglumine (Gd⋅DTPA), gadodiamide, gadoversetamide, gadobenate dimeglumine, gadoxetate disodium, gadofosveset trisodium, gadoteridol, gadobutrol, or gadoterate meglumine.

19. The device of claim 16 , wherein the paramagnetic ionic liquid comprises [methyltrioctylammonium] 2 [MnCl 4 ], [methyltrioctylammonium] 3 [GdCl 6 ], [methyltrioctylammonium] 3 [HoCl 6 ], [methyltrioctylammonium] 3 [HoBr 6 ], [1-butyl-3-methyl imidazolium]3 [HoCl 6 ], [1-butyl-3-methyl imidazolium][FeCl 4 ], [1-butyl-2,3-dimethyl imidazolium]2 [MnCl 4 ], [1-butyl-2,3-dimethyl imidazolium] 3 [DyCl 6 ], [1-butyl-3-methyl imidazolium]3 [DyCl 6 ], [alanine methylester][FeCl 4 ], [alanine methylester] 2 [MnCl 4 ], [alanine methylester]3 [GdCl 6 ], [alanine methylester] 3 [HoCl 6 ], or [alanine methylester] 3 [DyCl 6 ].

20. The device of claim 1 , wherein the one or more analytes comprise a diamagnetic material.

21. The device of claim 1 , wherein the one or more analytes comprise molecules, particles, powders, emulsions, biological samples, or biological cells.

22. A method comprising:
providing a device comprising:
a plate comprising one or more sample deposition tubes adapted to receive one or more samples comprising one or more analytes, each of the one or more sample deposition tubes defining a vertical axis substantially perpendicular to a main surface of the plate; and
one or more mirrors positioned to project, substantially parallel to the main surface of the plate, an image of each of the one or more sample deposition tubes along its vertical axis, so as to allow determination of the vertical location of the one or more analytes inside each of the one or more sample deposition tubes;
depositing a sample comprising one or more analytes into one or more of the sample deposition tubes;
allowing the one or more analytes to migrate to a position at least partially determined by its density; and
projecting, substantially parallel to the main surface of the plate, an image of each of the one or more sample deposition tubes along its vertical axis to determine the vertical location of the one or more analytes inside each of the one or more sample deposition tubes.

Assignees

• Harvard College

Inventors

• Ge Shencheng
• Wang Yunzhe
• Deshler Nicolas J
• Preston Daniel J
• Whitesides George M

Classifications

• G01N2021/1727

  Magnetomodulation · CPC title

• G01N21/1717

  with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance · CPC title

• G01N9/18 Primary

  Special adaptations for indicating, recording, or control · CPC title

• G01N21/253 Primary

  for batch operation, i.e. multisample apparatus (analytical automats G01N35/00) · CPC title