Human and non-human animal use of microbial anaplerotic oil

Patent metadata

Field	Value
Publication number	US-12285034-B2
Application number	US-202318364153-A
Country	US
Kind code	B2
Filing date	Aug 2, 2023
Priority date	Dec 22, 2017
Publication date	Apr 29, 2025
Grant date	Apr 29, 2025

Abstract

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

First claim

What is claimed is:

1. A method of incorporating saturated odd-chain fatty acids (OCFA) into food for human and non-human animal consumption, the method comprising:
replacing saturated fat of the food with microbial anaplerotic oil comprising the OCFA.

2. The method of claim 1 , wherein replacing the saturated fat comprises:
(a) identifying a unit amount of the saturated fat present in the food;
(b) determining an amount of the saturated fat for removal from the food;
(c) determining an amount of the microbial anaplerotic oil to incorporate into the food to replace the saturated fat to be removed from the food,
wherein determining the amount of the microbial anaplerotic oil comprises:
(i) identifying an amount of the OCFA present in the microbial anaplerotic oil as a percent of the microbial anaplerotic oil; and
(ii) determining the amount of the microbial anaplerotic oil to incorporate into the food based on the percent identified in step (c) (i) and the amount of the saturated fat to be removed from the food;
(d) removing the determined amount of the saturated fat from the food; and
(e) adding the determined amount of the microbial anaplerotic oil to the food.

3. The method of claim 2 , wherein the saturated fat is removed by a process selected from the group consisting of separation with a solvent, separation via filtration, and molecular distillation.

4. The method of claim 1 , wherein the saturated fat comprises even-chain fatty acids (ECFA).

5. The method of claim 4 , wherein the ECFA comprise tetradecanoic acid (C14:0), hexadecenoic acid (C16:0), octadecanoic acid (C18:0), or a combination thereof.

6. The method of claim 1 , wherein the OCFA comprise tridecanoic acid (C13:0), pentadecanoic acid (C15:0), heptadecanoic acid (C17:0), or a combination thereof.

7. The method of claim 1 , wherein the microbial anaplerotic oil further comprises docosahexaenoic acid (DHA).

8. The method of claim 1 , wherein the microbial anaplerotic oil further comprises eicosapentaenoic acid (EPA).

9. The method of claim 1 , wherein the food is a dairy product.

10. The method of claim 9 , wherein the dairy product is selected from the group consisting of milk, cheese, cream, yogurt, butter, and powder milk.

11. The method of claim 1 , wherein the food is an infant formula.

12. The method of claim 1 , wherein the microbial anaplerotic oil is derived from a microorganism from the class Labyrinthulomycetes.

13. The method of claim 12 , wherein the microorganism is a thraustochytrid.

14. A method of incorporating saturated odd-chain fatty acids (OCFA) into food for human and non-human animal consumption, the method comprising: identifying a target amount of the OCFA for incorporation into the food; and adding an amount of microbial anaplerotic oil containing the target amount of the OCFA to the food.

15. The method of claim 14 , wherein the OCFA comprise tridecanoic acid (C13:0), pentadecanoic acid (C15:0), heptadecanoic acid (C17:0), or a combination thereof.

16. The method of claim 14 , wherein the food is a naturally occurring food.

17. The method of claim 14 , wherein the food is a plant-based food.

18. The method of claim 17 , wherein the food is a substitute for a naturally occurring food, wherein the target amount of the OCFA is equal to an amount of the OCFA in the naturally occurring food.

19. The method of claim 17 , wherein the food is a substitute for a naturally occurring food, wherein the target amount of the OCFA exceeds an amount of the OCFA in the naturally occurring food.

20. The method of claim 14 , wherein the target amount of the OCFA is a targeted daily amount of the OCFA.

Assignees

• Heliae Dev Llc

Inventors

• Lamont Micheal
• Amezquita Ernult Magdalena
• Ganuza Taberna Eneko

Classifications

• C12R2001/89

  Algae {; Processes using algae} · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title

• C12P7/6409

  Fatty acids · CPC title

• C12N1/12

  Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title

• A23L13/43

  Addition of vegetable fats or oils; Addition of non-meat animal fats or oils; Addition of fatty acids · CPC title