Stevia cultivar ‘320032’ with super high rebaudioside A content

Patent metadata

Field	Value
Publication number	US-12284959-B2
Application number	US-202017761469-A
Country	US
Kind code	B2
Filing date	Sep 23, 2020
Priority date	Sep 24, 2019
Publication date	Apr 29, 2025
Grant date	Apr 29, 2025

Abstract

A stevia cultivar with super high Rebaudioside A content, designated ‘320032’, is disclosed. Another embodiment relates to the plant parts of stevia cultivar ‘320032’, to the plants of stevia ‘320032’ and to methods for producing a stevia plant produced by crossing the cultivar ‘320032’ with itself or another stevia variety, including methods using marker assisted breeding. Another embodiment further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘320032’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘320032’-derived plant materials are also disclosed.

First claim

What is claimed is:

1. A propagated plant of stevia cultivar ‘320032’, wherein a representative sample of plant tissue of said cultivar was deposited under CGMCC No. 16986.

2. The plant part of claim 1 , wherein said plant part is a seed, leaf, pollen, stem, root, an ovule, or a cell.

3. A stevia plant, or part thereof, having all of the molecular, physiological and morphological characteristics of the stevia plant of claim 1 .

4. A food or feed product comprising the plant or part thereof of claim 1 .

5. A tissue or cell culture of regenerable cells of the plant of claim 1 .

6. The tissue or cell culture of claim 1 , comprising tissues or cells from a plant part selected from the group consisting of leaves, pollen, embryos, cotyledons, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, and stems.

7. A stevia plant regenerated from the tissue or cell culture of claim 6 , wherein said plant has all of the molecular, morphological and physiological characteristics of stevia cultivar ‘320032’.

8. A method of vegetatively propagating the plant of claim 1 , comprising the steps of:
a. collecting tissue or cells capable of being propagated from a plant according to claim 1 ;
b. cultivating said tissue or cells of (a) to obtain proliferated shoots; and
c. rooting said proliferated shoots to obtain rooted plantlets; or
d. cultivating said tissue or cells to obtain proliferated shoots, or to obtain plantlets.

9. A stevia plant produced by growing the plantlets or proliferated shoots of claim 8 .

10. A method for producing an F 1 stevia seed, wherein the method comprises crossing the plant of claim 1 with a different stevia plant and harvesting the resultant F 1 stevia seed.

11. A stevia seed produced by the method of claim 10 .

12. A stevia plant produced by growing said seed of claim 11 .

13. A method of determining the genotype of the stevia plant of claim 1 , wherein said method comprises obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms.

14. A method of producing an herbicide resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene wherein the transgene confers resistance to an herbicide chosen from glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, cyclohexanedione, L-phosphinothricin, triazine, benzonitrile, and bromoxynil.

15. An herbicide resistant stevia plant produced by the method of claim 14 , wherein the plant has herbicide resistance and otherwise all of the physiological and morphological characteristics of stevia cultivar ‘320032’.

16. A method of producing an insect resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene that confers insect resistance.

17. An insect resistant stevia plant produced by the method of claim 16 , wherein the plant has insect resistance and otherwise all of the physiological and morphological characteristics of stevia cultivar ‘320032’.

18. The stevia plant of claim 17 , wherein the transgene encodes a Bacillus thuringiensis endotoxin.

19. A method of producing a disease resistant stevia plant, wherein the method comprises transforming the stevia plant of claim 1 with a transgene that confers disease resistance.

20. A disease resistant stevia plant produced by the method of claim 19 , wherein the plant has disease resistance and otherwise all of the physiological and morphological characteristics of stevia cultivar ‘320032’.

21. A method of introducing a desired trait into stevia cultivar ‘320032’, wherein the method comprises:
a. crossing a ‘320032’ plant, wherein a representative sample of plant tissue of said plant was deposited under CGMCC No. 16986, with a plant of another stevia cultivar that comprises a desired trait to produce progeny plants wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, modified carbohydrate metabolism, modified stevioside content, modified Rebaudioside content, modified stevia fiber characteristics, and resistance to bacterial disease, fungal disease or viral disease;
b. selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
c. crossing the selected progeny plants with the ‘320032’ plants to produce backcross progeny plants;
d. selecting for backcross progeny plants that have the desired trait and the physiological and morphological characteristics of stevia cultivar ‘320032’ to produce selected backcross progeny plants; and
e. repeating steps (c) and (d) two or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and otherwise all of the physiological and morphological characteristics of stevia cultivar ‘320032’ listed in Table 1.

22. A stevia plant produced by the method of claim 16 , wherein the plant has the desired trait and otherwise all of the physiological and morphological characteristics of stevia cultivar ‘320032’.

23. A method for developing a stevia plant in a stevia plant breeding program, comprising applying plant breeding techniques comprising recurrent selection, backcrossing, pedigree breeding, marker enhanced selection, haploid/double haploid production, or transformation to the stevia plant of claim 1 , or its parts, wherein application of said techniques results in development of a stevia plant.

24. A method for producing a sweetener composition containing steviol glycosides, which comprises extracting dried leaves of a Stevia variety ‘320032’ plant, produced by vegetative propagation of said plant deposited under CGMCC No. 16986, with water or an aqueous solvent to obtain an extract containing steviol glycosides, and wherein a sweetener composition is produced.

25. A sweetener composition of claim 24 , further combined with at least one acceptable food additive.

Assignees

• Purecircle Usa Inc

Inventors

• Markosyan Avetik
• Jing Runchun
• Ong Seong Siang
• Wang Chunhui
• Bu Yucheng
• Chen Jianning
• Wong Yeen Yee

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01H5/12

  Leaves · CPC title

• A23L27/36

  Terpene glycosides · CPC title