Genetically engineered microorganisms for the production of chorismate-derived products
US-10174303-B2 · Jan 8, 2019 · US
US12280331B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12280331-B2 |
| Application number | US-202217661523-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 29, 2022 |
| Priority date | Apr 29, 2022 |
| Publication date | Apr 22, 2025 |
| Grant date | Apr 22, 2025 |
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The systems and methods disclosed herein provide for the efficient separation of gas from fluid comprising liquid. In particular, gas separator systems and methods for use in bioreactor systems and operations are disclosed herein providing an efficient means to remove gaseous waste products, unreacted gaseous substrates, and or inert gas from broth of the biological fermentation of carbon substrates using a microorganism biocatalyst.
Opening claim text (preview).
The invention claimed is: 1. A method of separating gas from a liquid comprising: feeding a fluid comprising liquid and gas into at least one inlet of a separator vessel; channeling the fluid from the inlet into a fluid distributor positioned within the separator vessel; directing the fluid within the fluid distributor and uniformly irrigating, through orifices in the fluid distributor, a plurality of inclined plates positioned within the separator vessel and along a length of the fluid distributor; allowing the gas to rise against the plurality of inclined plates; draining the liquid through at least one liquid outlet; and removing the gas through at least one gas outlet. 2. The method of claim 1 , further comprising pressurizing the separator vessel, wherein the separator vessel is cylindrical. 3. The method of claim 2 , further comprising positioning the cylindrical separator vessel in a horizontal plane. 4. The method of claim 1 , wherein the uniformly irrigating the plurality of inclined plate comprises passing the fluid though a plurality of orifices positioned on the sides, a bottom, or both of the fluid distributor. 5. The method of claim 4 , wherein a first volume of fluid passes through orifices located above a top of the plurality of inclined plates and a second volume of fluid passes through orifices located below the top of the inclined plates. 6. The method of claim 5 , wherein the first volume of fluid irrigating the plurality of inclined plates comprises about 30% of a total liquid volume, and the second volume of fluid irrigating the plurality of inclined plates comprises about 70% of the total liquid volume. 7. The method of claim 1 , further comprising maintaining a constant cross-flow velocity and a constant bubble separation velocity across the plurality of inclined plates. 8. The method of claim 1 , further comprising recirculating the drained liquid to a reactor.
Drying gases or vapours · CPC title
Biogas · CPC title
Carbon dioxide · CPC title
Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title
Foam dispersion or prevention (during boiling B01B1/02; during fermentation C12) · CPC title
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