Method for making superparamagnetic iron oxide nanocarrier

Patent metadata

Field	Value
Publication number	US-12280020-B2
Application number	US-202318520628-A
Country	US
Kind code	B2
Filing date	Nov 28, 2023
Priority date	Aug 6, 2018
Publication date	Apr 22, 2025
Grant date	Apr 22, 2025

Abstract

Silica nanocarriers hybridized with superparamagnetic iron oxide nanoparticles (“SPIONs”) and curcumin through equilibrium or enforced adsorption technique. Methods for dual delivery of SPIONs and curcumin to a target for diagnosis or therapy, for example, for SPION-based magnetic resonance imaging or for targeted delivery of curcumin to a cell or tissue. The technique can be extend to co-precipitation of mixed metal oxide involving Ni, Mn, Co and Cu oxide. The calcination temperature can be varied from 500-900° C. The nanocombination is functionalized with chitosan, polyacrylic acid, PLGA or another agent to increase its biocompatibility in vivo.

First claim

The invention claimed is:

1. A method for making a superparamagnetic iron oxide nanocarrier, comprising:
drying a nanoporous structured silica under vacuum to form a platform of nanoporous structured silica;
impregnating nanostructured pore surfaces of the platform of nanoporous structured silica with superparamagnetic iron oxide nanoparticles (SPIONs) to form an impregnated platform of structured silica, wherein the impregnating includes:
mixing the platform of nanoporous structured silica with iron nitrate to form an iron precursor;
drying the iron precursor to form the impregnated platform of structured silica; and
calcining the impregnated platform of structured silica between 500 and 900° C. to form a calcined platform of structured silica and magnetic particles, and

adsorbing a curcuminoid onto the calcined platform of structure silica and magnetic nanoparticles to form the superparamagnetic iron oxide nanocarrier, wherein the adsorbing includes:
mixing the calcined platform of structured silica and magnetic nanoparticles with an aqueous solution of the curcuminoid,

wherein the superparamagnetic iron oxide nanocarrier comprises at least one nanoporous structured silica selected from the group consisting of SiSBA-16, Q-10 silica, mesocellular foam, silicalite, mesosilicalite, SiKIT-6, ULPFDU-12, SiMCM-41, ZSM-5, USY, Mordenite, ZSM-11, ZSM-12, ZSM-22, ZSM-23, and mixtures thereof,
wherein the superparamagnetic iron oxide nanoparticles are present in an amount ranging from about 5 wt % to about 30 wt % based on total weight of the superparamagnetic iron oxide nanocarrier, and
wherein the curcuminoid is adsorbed on the surface of the nanoporous structured silica.

2. The method of claim 1 , wherein the platform of nanoporous structured silica comprises at least one of MSU-foam, SiSBA-16, or Q-10; and wherein the curcuminoid is curcumin.

3. The method of claim 1 , wherein the platform of nanoporous structured silica comprises MSU-foam.

4. The method of claim 1 , wherein the platform of nanoporous structured silica comprises SiSBA-16.

5. The method of claim 1 , wherein the platform of nanoporous structured silica comprises Q-10.

6. The method of claim 1 , wherein the SPIONs comprise Fe 2 O 3 or a mixture of NiFe 2 O 4 , CuFe 2 O 4 , MnFe 2 O 4 and CoFe 2 O 4 .

7. The method of claim 1 , wherein the SPIONs comprise γ-Fe 2 O 3 .

8. The method of claim 1 , wherein the SPIONs have an average particle size ranging from about 7 to about 18 nm when the platform of nanoporous structured silica is MSU-foam; about 9 to 21 nm when the platform of nanoporous structured silica is SiSBA-16; or about 10 to about 25 nm when the platform of nanoporous structured silica is Q-10.

9. The method of claim 1 , wherein the curcuminoid is present in an amount ranging from 50 to 70 wt %.

10. The method of claim 1 , wherein the superparamagnetic iron oxide nanocarrier further comprises a polymer, wherein the SPIONs and/or the curcuminoid is covered with or incorporated into the polymer; and/or wherein one or more components of the composition is functionalized with chitosan, polyacrylic acid, PLGA, or another agent to increase its biocompatibility in vivo.

11. The method of claim 1 , wherein the superparamagnetic iron oxide nanocarrier further comprises at least one antibody or other targeting agent that binds to cancer cells, neoplasm cells, or tumor cells.

12. The method of claim 1 , wherein the superparamagnetic iron oxide nanocarrier has a degree of magnetization (M, emu/g) as measured by vibrating sample magnetometry (VSM) greater than an otherwise identical composition wherein the platform of nanoporous structured silica consists of SiSBA-16, Q-10 silica, mesocellular foam, silicalite, mesosilicalite, SiKIT-6, ULPFDU-12 or SiMCM-41.

13. The method of claim 1 , wherein the superparamagnetic iron oxide nanocarrier has a percentage of cumulative curcuminoid release, in phosphate buffered saline (PBS) at pH 5.6 and 37° C. over 72 hours, greater than an otherwise identical composition wherein the platform of nanoporous structured silica consists of SiSBA-16, Q-10 silica, mesocellular foam, silicalite, mesosilicalite, SiKIT-6, ULPFDU-12 or SiMCM-41.

Assignees

• Univ Imam Abdulrahman Bin Faisal

Inventors

• Jermy B Rabindran
• Ravinayagam Vijaya

Classifications

• A61K47/6883

  Polymer-drug antibody conjugates, e.g. mitomycin-dextran-Ab; DNA-polylysine-antibody complex or conjugate used for therapy · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61K47/6929

  the form being a nanoparticle, e.g. an immuno-nanoparticle · CPC title

• A61K47/6923

  the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title