System for washing red blood cells to reduce hemolysis

Patent metadata

Field	Value
Publication number	US-12279609-B2
Application number	US-202217589454-A
Country	US
Kind code	B2
Filing date	Jan 31, 2022
Priority date	Jun 1, 2015
Publication date	Apr 22, 2025
Grant date	Apr 22, 2025

Abstract

A system for washing red blood cells, comprising a separator configured to separate a quantity of blood into concentrated red blood cells having a hematocrit of at least 60% and a volume of 150-250 mL, and a supernatant component. The system comprises a flow controller configured to remove the supernatant component to provide an initial red blood cell concentrate; combine 50-500 mL of an additive solution with the red blood cell concentrate to provide an intermediate red blood cell product intended for storage for 42 days or less; and wash the intermediate red blood cell product with a washing solution.

First claim

The invention claimed is:

1. A system for washing red blood cells, comprising:
a separator configured to separate a quantity of blood into concentrated red blood cells and a supernatant component;
a flow controller in communication with the separator, wherein the flow controller is configured to:
remove said supernatant component to provide an initial red blood cell concentrate;
combine an additive solution with said red blood cell concentrate to provide an intermediate red blood cell product that is intended for storage for a period of time; and
wash the intermediate red blood cell product that has been stored for a period of time with a washing solution.

2. The system of claim 1 , wherein the quantity of blood is approximately 400-500 mL in volume, the intermediate red blood cell product has a hematocrit of at least 60%, and the volume of additive solution added to the red blood cell concentrate is between 50 and 500 mL.

3. The system of claim 1 , wherein the period of time for storing the intermediate red blood cell product is not greater than 42 days.

4. The system of claim 1 , wherein the step of separating the quantity of blood into concentrated red blood cells and a supernatant component is performed by at least one of a centrifuge and spinning membrane.

5. The system of claim 1 , wherein extracellular component levels within the washed intermediate red blood cell product is substantially similar to the levels within the unwashed intermediate red blood cell product.

6. The system of claim 1 , wherein the additive solution comprises glucose, mannitol, and adenine.

7. A system for processing blood cells comprising:
a flow controller configured to add a selected volume of additive solution to a quantity of blood;
a separator in communication with the flow controller configured to separate the quantity of blood into concentrated red blood cells and a supernatant component, wherein the supernatant component comprises at least plasma and additive solution, and wherein the concentrated red blood cell component comprises red blood cells and a volume of remaining supernatant;
wherein the flow controller is configured to wash the concentrated red blood cell component with a wash media, and remove substantially all of the wash media and remaining supernatant to provide a treated red blood cell concentrate;
wherein a percentage of red cells that have undergone hemolysis during washing and removal of wash media is less than 0.3% of the red cells from the concentrated red blood cell component.

8. The system of claim 7 , wherein the quantity of blood is approximately 400-500 mL in volume, the intermediate red blood cell product has a hematocrit of at least 60%, and the volume of additive solution added to the red blood cell concentrate is between 50 and 500 mL.

9. The system of claim 7 , wherein the separation of the quantity of blood into concentrated red blood cells and a supernatant component is performed by at least one of a centrifuge and spinning membrane.

10. The system of claim 7 , wherein extracellular component levels within the treated red blood cell concentrate is substantially similar to the levels within the quantity of blood.

11. The system of claim 7 , wherein the additive solution comprises glucose, mannitol, and adenine.

12. A method for processing blood cells comprising:
adding a selected volume of additive solution to a quantity of blood;

separating the quantity of blood into concentrated red blood cells and a supernatant component, wherein the supernatant component comprises at least plasma and additive solution, and wherein the concentrated red blood cell component comprises red blood cells and a volume of remaining supernatant;
washing the concentrated red blood cell component with a wash media;

removing substantially all of the wash media and remaining supernatant to provide a treated red blood cell concentrate;
wherein the percentage of red cells that have undergone hemolysis during washing and removal of wash media is less than 0.3% of the red cells from the concentrated red blood cell component.

13. The method of claim 12 , wherein the quantity of blood is approximately 400-500 mL in volume, the intermediate red blood cell product has a hematocrit of at least 60%, and the volume of additive solution added to the red blood cell concentrate is between 50 and 500 mL.

14. The method of claim 12 , wherein the separation of the quantity of blood into concentrated red blood cells and a supernatant component is performed by at least one of a centrifuge and spinning membrane.

15. The method of claim 12 wherein extracellular component levels within the treated red blood cell concentrate is substantially similar to the levels within the quantity of blood.

16. The method of claim 12 , wherein the additive solution comprises glucose, mannitol, and adenine.

17. A method for washing red blood cells in the system of claim 1 comprising establishing flow communication between a source of said quantity of blood and said separator.

18. The method of claim 17 comprising collecting said intermediate red blood cell product in an intermediate product container of said processing set.

19. The method of claim 18 comprising rinsing said processing set after said collecting of said intermediate red blood cell product.

20. The method of claim 19 comprising removing said supernatant by compressing said intermediate container in a blood component extractor.

Assignees

• Fenwal Inc

Inventors

• Wegener Christopher J

Classifications

• C12N5/0634

  Cells from the blood or the immune system · CPC title

• A61M1/3693

  using separation based on different densities of components, e.g. centrifuging · CPC title

• A61K35/14

  Blood; Artificial blood (perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42) · CPC title

• A61M1/3692

  Washing or rinsing blood or blood constituents · CPC title

• A61M1/3609

  Physical characteristics of the blood, e.g. haematocrit, urea · CPC title