Methods for predicting the risk of developing pulmonary colonization/infection by Pseudomonas aeruginosa

Patent metadata

Field	Value
Publication number	US-12276000-B2
Application number	US-201917043100-A
Country	US
Kind code	B2
Filing date	Mar 28, 2019
Priority date	Mar 29, 2018
Publication date	Apr 15, 2025
Grant date	Apr 15, 2025

Abstract

The present invention relates to methods for predicting the risk of developing pulmonary colonization/infection by P. aeruginosa . The inventors analyzed the respiratory tract microbiota from 65 patients sputum samples and compared microbiota data. The inventors found that patients that will remain uninfected from P. aeruginosa exhibited 3-fold higher abundance of Porphyromonas catoniae compared to the other groups. In particular, the present invention relates to a method for predicting the risk of developing pulmonary colonization/infection by P. aeruginosa in a subject suffering from cystic fibrosis (CF) comprising measuring the abundance of Porphyromonas catoniae in a biological sample obtained from said subject.

First claim

The invention claimed is:

1. A method of preventing pulmonary colonization/infection by P. aeruginosa (Pa) in a subject suffering from cystic fibrosis (CF) comprising:
predicting the risk of developing pulmonary colonization/infection by P. aeruginosa by measuring the absolute abundance of Porphyromonas catoniae at different times in biological samples obtained from said subject, wherein no bacterial species other than Porphyromonas catoniae is measured;
detecting a decrease over time in the absolute abundance of Porphyromonas catoniae in the subject, thus the subject having a high risk of developing pulmonary colonization/infection by Pseudomonas aeruginosa ; and
administering to the subject a therapeutically effective amount of P. aeruginosa specific antibiotics.

2. The method of claim 1 , wherein the subject is a child under 12 months of age.

3. The method of claim 1 , wherein the biological sample is bronchoalveolar lavage (BAL) or sputum.

4. The method of claim 1 , wherein the abundance of Porphyromonas genus bacteria is measuring using 16S rRNA deep-sequencing.

Assignees

• Inst Nat Sante Rech Med
• Centre Hospitalier Regional Et Univ De Brest
• Univ Bretagne Occidentale
• Institut National De Rech Pour Lagriculture Lalimentation Et Lenvironnement
• Etablissement Francais Du Sang Efs
• Univ Bretagne Occidentale
• Institut National De Rech Pour Lagriculture Lalimentation Et Lenvironnment

Inventors

• Hery-Arnaud Geneviève
• Mounier Jérôme
• Guilloux Charles-Antoine
• Lepage Patricia
• Mondot Stanislas
• Keravec Marlène

Classifications

• C12Q2600/118

  Prognosis of disease development · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12Q1/689 Primary

  for bacteria · CPC title

• C12Q1/6883 Primary

  for diseases caused by alterations of genetic material · CPC title