Engineered nucleic acids and methods of use thereof
US-9701965-B2 · Jul 11, 2017 · US
In vitro method of reprogramming fibroblast cells into induced pluripotent stem cells by transcription factors mRNAs co-transfected with SOCS1 mRNA wherein the mRNAs have a poly-A tail of at least 2000 adenines
US12275950B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12275950-B2 |
| Application number | US-201716099134-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 2, 2017 |
| Priority date | May 6, 2016 |
| Publication date | Apr 15, 2025 |
| Grant date | Apr 15, 2025 |
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Abstract
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The present invention provides the use of a nucleic acid encoding SOCS1 for enhancing the efficacy of introducing at least one nucleic acid of interest into a cell; a method of repeated transfection of a cell with at least one nucleic acid of interest comprising the steps of adding a) nucleic acid encoding SOCS1, and simultaneously or subsequently b) at least one nucleic acid of interest encoding at least one polypeptide of interest, wherein at least step b) is repeated at least once; and a method of electroporation of a cell with at least one nucleic acid of interest comprising the steps of adding to the cell a) a nucleic acid encoding SOCS1, and simultaneously or subsequently b) said at least one nucleic of interest. The at least one nucleic acid of interest and the nucleic acid encoding SOCS1 may be mRNAs, wherein each of said mRNAs has a poly(A) tail at its 3′ end comprising at least 200 adenines.
First claim
Opening claim text (preview).
The invention claimed is: 1. An in vitro method of repeated transfection of a fibroblast cell with at least one mRNA encoding a reprogramming factor, comprising introducing to the fibroblast cell: a) Suppressor Of Cytokine Signaling 1 (SOCS1) mRNA; and simultaneously or subsequently b) the at least one mRNA encoding a reprogramming factor; wherein the step b) is repeated at least once, and wherein the SOCS1 mRNA and the at least one mRNA encoding the reprogramming factor have a poly (A) tail at its 3′ end comprising at least 2000 adenines, respectively, wherein the reprogramming factor induces reprograming of the fibroblast cell into an induced pluripotent stem cell (iPSC), wherein the at least one mRNA encoding the reprogramming factor is an mRNA reprogramming mixture comprising mRNAs encoding for Oct3/4, Sox2, Klf4, Lin28, c-Myc, and Nanog. 2. The method according to claim 1 , wherein the step b) is repeated at least three times. 3. The method according to claim 2 , wherein the step b) is repeated within 4 hours to 48 hours between a first time and a second time. 4. The method according to claim 1 , wherein the method results in an increased expression of the mRNA encoding the reprogramming factor compared to a corresponding method wherein the SOCS1 mRNA is not co-introduced. 5. The method according to claim 4 , wherein the method results in an increased reprogramming efficiency of the fibroblast cell compared to a corresponding method wherein the SOCS1 mRNA is not co-introduced.
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Classifications
Artificially induced pluripotent stem cells, e.g. iPS · CPC title
Cells modified by introduction of foreign genetic material · CPC title
T lymphocytes · CPC title
Stem cells · CPC title
Inhibitors; Suppressors · CPC title
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- What does patent US12275950B2 cover?
- The present invention provides the use of a nucleic acid encoding SOCS1 for enhancing the efficacy of introducing at least one nucleic acid of interest into a cell; a method of repeated transfection of a cell with at least one nucleic acid of interest comprising the steps of adding a) nucleic acid encoding SOCS1, and simultaneously or subsequently b) at least one nucleic acid of interest encodi…
- Who is the assignee on this patent?
- Miltenyi Biotec Gmbh, Miltenyi Biotec Bv & Co Kg
- What technology area does this patent fall under?
- Primary CPC classification C12N15/87. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 15 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).