Devices, systems, and methods for increasing droplet formation efficiency

Patent metadata

Field	Value
Publication number	US-12269036-B2
Application number	US-202117458757-A
Country	US
Kind code	B2
Filing date	Aug 27, 2021
Priority date	Feb 28, 2019
Publication date	Apr 8, 2025
Grant date	Apr 8, 2025

Abstract

Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

First claim

What is claimed is:

1. A device for producing droplets of a first liquid in a second liquid, the device comprising:
a) a sample inlet;
b) first, second, and third sample channels, each of which is in fluid communication with the sample inlet;
c) first and second reagent inlets;
d) first, second, and third reagent channels, wherein the first and second reagent channels are in fluid communication with the first reagent inlet; the third reagent channel is in fluid communication with the second reagent inlet; the first sample channel and the first reagent channel intersect at a first intersection, the second sample channel and the second reagent channel intersect at a second intersection, and the third sample channel and the third reagent channel intersect at a third intersection;
e) first, second, and third droplet formation regions; and
f) first and second collection reservoirs,

wherein the first droplet formation region is fluidically disposed between the first intersection and the first collection reservoir, the second droplet formation region is fluidically disposed between the second intersection and the first collection reservoir, and the third droplet formation region is fluidically disposed between the third intersection and the second collection reservoir.

2. The device of claim 1 , further comprising i) a fourth sample channel in fluid communication with the sample inlet; ii) a fourth reagent channel in fluid communication with the second reagent inlet, and iii) a fourth droplet formation region, wherein the fourth sample channel and fourth reagent channel intersect at a fourth intersection, and the fourth droplet formation region is fluidically disposed between the fourth intersection and the second collection reservoir.

3. The device of claim 1 , wherein the inlets are arranged substantially linearly.

4. The device of claim 1 , wherein the first, second, and third sample channels are co-planar.

5. The device of claim 1 , wherein the first, second, and third reagent channels are co-planar.

6. The device of claim 1 , wherein the length of the first reagent channel is at least 85% of the length of the second reagent channel.

7. The device of claim 2 , wherein the length of the third reagent channel is at least 85% of the length of the fourth reagent channel.

8. The device of claim 1 , wherein the first and second collection reservoirs and the first and second reagent inlets are disposed radially about the sample inlet.

9. The device of claim 2 , comprising a plurality of first and second reagent inlets; sample inlets; first and second collection reservoirs; first, second, third, and fourth sample channels; first, second, third, and fourth reagent channels; first, second, third, and fourth intersections; and first, second, third, and fourth droplet formation regions.

10. The device of claim 9 , wherein:
a) at least two of the plurality of sample inlets are in fluid communication with each other via a connecting channel; or
b) a sample channel in fluid communication with one of the plurality of sample inlets and one of the plurality of collection reservoirs intersects with a sample channel in fluid communication with a separate one of the plurality of sample inlets and a separate one of the plurality of collection reservoirs.

11. The device of claim 1 , wherein at least one of the droplet formation regions comprises a shelf that allows a liquid to expand in one dimension and a step that allows the liquid to expand in an orthogonal dimension.

12. The device of claim 9 , further comprising one or more troughs, wherein the one or more troughs connects the plurality of sample inlets, the plurality of first reagent inlets, and/or connects the plurality of second reagent inlets.

13. A device for producing droplets, the device comprising:
a) a sample inlet;
b) first, second, third, and fourth sample channels, each of which is in fluid communication with the sample inlet;
c) first and second reagent inlets;
d) first, second, third, and fourth reagent channels, wherein the first and second reagent channels are in fluid communication with the first reagent inlet; wherein the third and fourth reagent channels are in fluid communication with the second reagent inlet; wherein the first sample channel and the first reagent channel intersect at a first intersection, the second sample channel and the second reagent channel intersect at a second intersection, the third sample channel and the third reagent channel intersect at a third intersection, and the fourth sample channel and the fourth reagent channel intersect at a fourth intersection;
e) first, second, third, and fourth droplet formation regions; and
f) a collection reservoir,

wherein the first droplet formation region is fluidically disposed between the first intersection and the collection reservoir, the second droplet formation region is fluidically disposed between the second intersection and the collection reservoir, the third droplet formation region is fluidically disposed between the third intersection and the collection reservoir, and the fourth droplet formation region is fluidically disposed between the fourth intersection and the collection reservoir.

14. The device of claim 13 , further comprising:
g) a third reagent inlet;
h) fifth and sixth sample channels, each of which is in fluid communication with the sample inlet;
i) fifth and sixth reagent channels, each of which is in fluid communication with the third reagent inlet, wherein the fifth sample channel and the fifth reagent channel intersect at a fifth intersection, and the sixth sample channel and sixth reagent channel intersect at a sixth intersection; and
j) fifth and sixth droplet formation regions,

wherein the fifth droplet formation region is fluidically disposed between the fifth intersection and the collection reservoir, and the sixth droplet formation region is fluidically disposed between the sixth intersection and the collection reservoir.

15. The device of claim 14 , further comprising:
k) a fourth reagent inlet;
l) seventh, and eighth sample channels, each of which is in fluid communication with the sample inlet;
m) seventh, and eighth reagent channels, each of which is in fluid communication with the reagent inlet, wherein the fifth sample channel and the fifth reagent channel intersect at a fifth intersection, the sixth sample channel and sixth reagent channel intersect at a sixth intersection, the seventh sample channel and the seventh reagent channel intersect at a seventh intersection, and the eighth sample channel and the eighth reagent channel intersect at an eighth intersection
n) fifth, sixth, seventh, and eighth droplet formation regions,

wherein the seventh droplet formation region is fluidically disposed between the seventh intersection and the collection reservoir, and the eighth droplet formation region is fluidically disposed between the eighth intersection and the collection reservoir.

16. The device of claim 13 , further comprising at least one trough connecting at least two of the reagent inlets.

17. The device of claim 13 , wherein the sample inlet, first through fourth sample channels, first and second reagent inlets, first through fourth reagent channels, first through fourth intersections, and first through fourth droplet formation regions define a flow path and wherein the device comprises a plurality of flow paths disposed radially about the sample inlet.

18. The device of claim 15 , wherein the sample inlet, first through eighth sample channels, first thr

Assignees

• 10X Genomics Inc

Inventors

• Akhremichev Ivan
• Bharadwaj Rajiv
• Chen Lynna
• Rahimi Lenji Mohammad
• Salmanzadeh Alireza
• Sauzade Martin
• Wheeler Tobias Daniel
• Zhang Yiran

Classifications

• G01N15/1492

  within droplets · CPC title

• G01N15/149

  specially adapted for sorting particles, e.g. by their size or optical properties · CPC title

• B01F23/4144

  Multiple emulsions, in particular double emulsions, e.g. water in oil in water; Three-phase emulsions · CPC title

• B01F33/3011

  using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream · CPC title

• B01F25/31423

  with a plurality of perforations in the circumferential direction only and covering the whole circumference · CPC title