Urinary system catheter using ethanol extract of propolis as coating and preparation method thereof

Patent metadata

Field	Value
Publication number	US-12263313-B2
Application number	US-202418733907-A
Country	US
Kind code	B2
Filing date	Jun 5, 2024
Priority date	Aug 25, 2023
Publication date	Apr 1, 2025
Grant date	Apr 1, 2025

Abstract

A urinary system catheter is covered by an ethanol extract of propolis on a surface of the urinary system catheter. The EEP is used as a coating material of the urinary system catheter to prepare an antimicrobial urinary system catheter. The EEP serving as a surface coating can enhance surface hydrophilicity of the urinary system catheter, and clinical operation of the urinary system catheter is facilitated. Meanwhile, the EEP serving as the surface coating can prevent a bacterial biofilm from being formed on the surface of the urinary system catheter, thereby possessing antimicrobial and anti-infective effects, and a high anti-scab capacity. In addition, propolis is a natural substance, has the advantages of good biocompatibility and low cost, and is suitable for mass production. The urinary system catheter prepared by using the EEP as the coating has a good application prospect.

First claim

What is claimed is:

1. An antimicrobial and anti-infective urinary system catheter, comprising:
a surface of a urinary system catheter; and
a coating of a propolis alcohol extract directly covered on the surface of the urinary system catheter, to enhance hydrophilicity of the surface of the urinary system catheter, prevent stones and scabs forming on the surface of the urinary system catheter, and make the urinary system catheter antimicrobial and anti-infective;
wherein the propolis alcohol extract is an ethanol extract of propolis, and a preparation method of the ethanol extract of propolis comprises the following steps: adding ethanol into propolis to perform ultrasonic extraction to obtain an extract, and filtering the extract to obtain supernatant, thereby obtaining the ethanol extract of propolis;
wherein the ethanol is an ethanol solution with a volume percentage of 75%;
wherein a mass of the ethanol is 25 times of a mass of the propolis;
wherein a temperature of the ultrasonic extraction is 60 degrees Celsius (° C.), a frequency of the ultrasonic extraction is 37 kilohertz (kHz), a power of the ultrasonic extraction is 100 watts (W), and a time for the ultrasonic extraction is 60 minutes (min); and
wherein a method for covering the surface of the urinary system catheter with the coating of the propolis alcohol extract comprises the following steps: immersing the urinary system catheter into a solution of the ethanol extract of propolis, and then taking out and drying to obtain the antimicrobial and anti-infective urinary system catheter;
wherein a solvent of the solution of the ethanol extract of propolis is the ethanol with the volume percentage of 75%, and a concentration of the solution of the ethanol extract of propolis is in a range of 32 milligrams per milliliter (mg/mL) to 128 mg/mL;
wherein the coating of the propolis alcohol extract comprises: total flavonoids and total phenolic acid, and an average surface propolis content of the coating of the propolis alcohol extract is greater than or equal to 0.721 according to the following formula for calculating the average surface propolis content:

total
⁢

flavonoids
⁢

content
⁢

in
⁢

solution
/
274.4

+

total
⁢

phenolic
⁢

acid
⁢

content
⁢

in
⁢

solution
/
143.7

2
×
surface
⁢

area
⁢

of
⁢

segment

where total flavonoids content in solution represents content of the total flavonoids in a dissolved solution of the coating of the propolis alcohol extract, total phenolic acid content in solution represents content of the total phenolic acid in the dissolved solution of the coating of the propolis alcohol extract, surface area of segment represents an area of the surface of the urinary system catheter coated with the coating of the propolis alcohol extract; and
wherein a contact angle between the coating of the propolis alcohol extract covered on the surface of the urinary system catheter and water is less than 90°.

2. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a ureteral stent, a catheter, a urethral stent, a prostate stent, a cystostomy catheter, a nephrostomy catheter, or a urological guidewire.

3. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the contact angle between the coating of the propolis alcohol extract covered on the surface of the urinary system catheter and the water is less than 60°.

4. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the total flavonoids is 274.4±4.4 mg/g, and the total phenolic acid is 143.7±0.3 mg/g.

5. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a ureteral stent, a concentration of the propolis alcohol extract is 32 mg/mL, and the average surface propolis content is 0.721 mg/cm 2 .

6. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a ureteral stent, a concentration of the propolis alcohol extract is 64 mg/mL, and the average surface propolis content is 1.598 mg/cm 2 .

7. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a ureteral stent, a concentration of the propolis alcohol extract is 128 mg/mL, and the average surface propolis content is 4.072 mg/cm 2 .

8. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a catheter, a concentration of the propolis alcohol extract is 32 mg/mL, and the average surface propolis content is 1.163 mg/cm 2 .

9. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a catheter, a concentration of the propolis alcohol extract is 64 mg/mL, and the average surface propolis content is 4.134 mg/cm 2 .

10. The antimicrobial and anti-infective urinary system catheter as claimed in claim 1 , wherein the urinary system catheter is a catheter, a concentration of the propolis alcohol extract is 128 mg/mL, and the average surface propolis content is 6.451 mg/cm 2 .

Assignees

• Univ Sichuan

Inventors

• Yang Luo

Classifications

• B01D11/0265

  Applying ultrasound · CPC title

• B01D11/0288

  Applications, solvents · CPC title

• C09D193/04

  Rosin · CPC title

• A61L2300/30

  Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera · CPC title

• A61L2300/404

  Biocides, antimicrobial agents, antiseptic agents · CPC title