Limiting yeast-produced trehalose in fermentation

Patent metadata

Field	Value
Publication number	US-12258606-B2
Application number	US-202318183669-A
Country	US
Kind code	B2
Filing date	Mar 14, 2023
Priority date	Nov 6, 2015
Publication date	Mar 25, 2025
Grant date	Mar 25, 2025

Abstract

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

First claim

What is claimed is:

1. A method of increasing the production of a fermentation product during a fermentation, said method comprising contacting a medium with (i) a recombinant yeast host cell comprising a first genetic modification for expressing a heterologous glucose-inactivated glycerol proton symporter STL1 (STL1) protein and (ii) a trehalase, wherein the production of the fermentation product is increased when compared to a control method with a control yeast host cell lacking the first genetic modification.

2. The method of claim 1 , wherein the recombinant yeast host cell comprises a further genetic modification for reducing the production of one or more native enzymes that function to produce glycerol.

3. The method of claim 2 , wherein the recombinant yeast host cell expresses less of a native glycerol-3-phosphate dehydrogenase 1 (GPD1) polypeptide than a control yeast host cell lacking the further genetic modification.

4. The method of claim 2 , wherein the recombinant yeast host cell lacks the ability to produce a native GPD1 polypeptide.

5. The method of claim 2 , wherein the recombinant yeast host cell expresses less of a native glycerol-3-phosphate dehydrogenase 2 (GPD2) polypeptide than a control yeast host cell lacking the further genetic modification.

6. The method of claim 2 , wherein the recombinant yeast host cell lacks the ability to produce a native GPD2 polypeptide.

7. The method of claim 1 , wherein the recombinant yeast host cell is from the genus Saccharomyces sp.

8. The method of claim 7 , wherein the recombinant yeast host cell is from the species Saccharomyces cerevisiae.

9. The method of claim 1 , wherein the trehalase is extracellular from the recombinant yeast host cell.

10. The method of claim 1 , wherein the trehalase is provided in a purified form.

11. The method of claim 1 , wherein the trehalase belongs to E.C. 3.1.2.1.28.

12. The method of claim 1 , wherein the trehalase is an acid trehalase.

13. The method of claim 1 , wherein the trehalase is a neutral trehalase.

14. The method of claim 1 , wherein the medium comprises trehalose.

15. The method of claim 1 , wherein the medium comprises starch.

16. The method of claim 15 , wherein the starch is provided in a gelatinized or a raw form.

17. The method of claim 1 , wherein the medium is derived from corn.

Assignees

• Lallemand Hungary Liquidity Man Llc
• Danstar Ferment Ag

Inventors

• Rice Charles F
• Skinner Ryan
• Barrett Trisha
• Argyros Aaron

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P19/12

  Disaccharides · CPC title

• C12P7/20

  Glycerol · CPC title

• C12N15/81

  for yeasts · CPC title

• C12N1/18

  Baker's yeast; Brewer's yeast · CPC title