Sample preliminary screening chip, specimen detecting method, and screening device

Patent metadata

Field	Value
Publication number	US-12257582-B2
Application number	US-202016898516-A
Country	US
Kind code	B2
Filing date	Jun 11, 2020
Priority date	Dec 18, 2019
Publication date	Mar 25, 2025
Grant date	Mar 25, 2025

Abstract

The present disclosure discloses a sample preliminary screening chip, a specimen detecting method and a screening device. A data processor may be configured to control a sample solution containing a specimen to be added into a preliminary screening inlet of the sample preliminary screening chip, and control the sample solution in the preliminary screening inlet to enter a channel, successively to flow through a first preliminary screening area and a second preliminary screening area, and to flow out from a preliminary screening outlet so as to store a liquid with the specimen in a first preliminary screening area. In this way, the liquid containing the specimen may be screened preliminarily.

First claim

What is claimed is:

1. A specimen detecting method, comprising:
controlling, by a data processor, a sample solution containing a specimen to be added into a preliminary screening inlet of a sample preliminary screening chip; wherein the sample preliminary screening chip comprises:
a first preliminary screening substrate and a second preliminary screening substrate fitted to each other;
a groove structure of the first preliminary screening substrate facing the second preliminary screening substrate;
wherein the groove structure forms a channel encapsulated between the first preliminary screening substrate and the second preliminary screening substrate, and the channel is provided with two ends; and
the preliminary screening inlet in one of the two ends of the channel, and a preliminary screening outlet in the other one of the two ends of the channel; wherein
the preliminary screening inlet and the preliminary screening outlet run through the second preliminary screening substrate; the groove structure comprises a first groove structure and a second groove structure, the first groove structure is provided with a first depth in a direction perpendicular to a plane on which the first preliminary screening substrate is located, and the second groove structure is provided with a second depth in the direction perpendicular to the plane on which the first preliminary screening substrate is located; the channel is a preliminary screening area; in a direction from the preliminary screening inlet to the preliminary screening outlet, the preliminary screening area is divided into a first preliminary screening sub-area and a second preliminary screening sub-area; the first groove structure forms the first preliminary screening sub-area and the second groove structure forms the second preliminary screening sub-area; and
the first depth is greater than a maximum particle diameter of the specimen contained in the sample solution, and the second depth is smaller than a minimum particle diameter of the specimen;

controlling, by the data processor, the sample solution in the preliminary screening inlet to enter the channel, and controlling, by the data processor, a part of the sample solution to flow successively through the first preliminary screening sub-area and the second preliminary screening sub-area, and to flow out from the preliminary screening outlet so as to store a liquid with the specimen in the first preliminary screening sub-area;
controlling, by the data processor, the liquid in the first preliminary screening sub-area to flow into a liquid storage tank of a microfluidic chip;
controlling, by the data processor, the microfluidic chip to separate micro-droplets from a liquid in the liquid storage tank, wherein the micro-droplets are independent and micro-sized, a size of each of the micro-droplets is the sum of a maximum particle diameter of the specimen and a preset value;
acquiring, by the data processor, Raman spectra corresponding to each of the micro-droplets; and
controlling, by the data processor, the microfluidic chip to move at least one micro-droplet, among the micro-droplets, with the specimen into a specimen storage tank according to the Raman spectra corresponding to each of the micro-droplets.

2. The specimen detecting method according to claim 1 , wherein the controlling, by the data processor, the microfluidic chip to move the at least one micro-droplet with the specimen into a specimen storage tank according to the Raman spectra corresponding to each of the micro-droplets comprises:
acquiring, by the data processor, characteristic peak data in the Raman spectra corresponding to each of the micro-droplets;
determining for each of the micro-droplets, by the data processor, whether the each micro-droplet contains a specimen, by comparing the characteristic peak data corresponding to the each micro-droplet with a pre-stored corresponding relationship between a specimen and characteristic peak data; and
controlling, by the data processor, the microfluidic chip to move the each micro-droplet with the specimen into a specimen storage tank in response to the each micro-droplet containing the specimen.

3. The specimen detecting method according to claim 2 , wherein the controlling, by the data processor, the microfluidic chip to move the each micro-droplet with the specimen into a specimen storage tank in response to the each micro-droplet containing the specimen comprises:
generating, by the data processor, a first level signal in response to the each micro-droplet containing the specimen, wherein the first level signal is a signal used for controlling the microfluidic chip to move the each micro-droplet with the specimen into the specimen storage tank; and
providing, by the data processor, the first level signal to the microfluidic chip.

4. The specimen detecting method according to claim 2 , further comprising:
generating, by the data processor, a second level signal in response to the each micro-droplet not containing the specimen, wherein the second level signal is a signal used for controlling the microfluidic chip to move the each micro-droplet into a non-specimen storage tank; and
providing, by the data processor, the second level signal to the microfluidic chip.

5. The specimen detecting method according to claim 3 , further comprising:
generating, by the data processor, a second level signal in response to the each micro-droplet not containing the specimen, wherein the second level signal is a signal used for controlling the microfluidic chip to move the each micro-droplet into a non-specimen storage tank; and
providing, by the data processor, the second level signal to the microfluidic chip.

6. The specimen detecting method according to claim 1 , wherein the acquiring, by the data processor, Raman spectra corresponding to each of the micro-droplets comprises:
controlling, by the data processor, a Raman spectrometer with a set laser wavelength to scan each of the micro-droplets; and
collecting, by the data processor, the Raman spectra corresponding to each of the micro-droplets.

7. The specimen detecting method according to claim 2 , wherein the acquiring, by the data processor, Raman spectra corresponding to each of the micro-droplets comprises:
controlling, by the data processor, a Raman spectrometer with a set laser wavelength to scan each of the micro-droplets; and
collecting, by the data processor, the Raman spectra corresponding to each of the micro-droplets.

8. The specimen detecting method according to claim 3 , wherein the acquiring, by the data processor, Raman spectra corresponding to each of the micro-droplets comprises:
controlling, by the data processor, a Raman spectrometer with a set laser wavelength to scan each of the micro-droplets; and
collecting, by the data processor, the Raman spectra corresponding to each of the micro-droplets.

9. The specimen detecting method according to claim 1 , wherein after the controlling, by the data processor, the microfluidic chip to move the at least one micro-droplet with the specimen into the specimen storage tank according to the Raman spectra corresponding to each of the micro-droplets, the method further comprises:
controlling, by the data processor, a detecting device to detect a specimen in the specimen storage tank.

10. The specimen detecting method according to claim 2 , wherein after the controlling, by the data processor, the microfluidic chip to move the at least one micro-droplet with the specimen into the specimen storage tank according to the Raman spectra corresponding to each of the micro-droplets, the method further comprises:
controlling, by the data processor, a detecting device to detect a specimen

Assignees

• Beijing Boe Sensor Technology Co Ltd
• Boe Technology Group Co Ltd

Inventors

• Liao Hui
• Zhao Yingying
• Yao Wenliang
• Fan Bolin
• Zhao Nan
• Gu Le
• Gao Yongjia

Classifications

• G01N21/65

  Raman scattering · CPC title

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• B01L2200/143

  Quality control, feedback systems · CPC title

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• G01N15/149

  specially adapted for sorting particles, e.g. by their size or optical properties · CPC title