Detection apparatus and detection method

Patent metadata

Field	Value
Publication number	US-12247975-B2
Application number	US-202117162443-A
Country	US
Kind code	B2
Filing date	Jan 29, 2021
Priority date	Jun 30, 2016
Publication date	Mar 11, 2025
Grant date	Mar 11, 2025

Abstract

Disclosed is a detection apparatus that transfers magnetic particles through a plurality of chambers in a cartridge which includes the plurality of chambers and a channel connecting between the plurality of chambers, and that causes the magnetic particles to carry a complex of a test substance and a labelling substance, to detect the test substance on the basis of the labelling substance in the complex. The detection apparatus includes: a rotation mechanism configured to rotate the cartridge about a rotation shaft; a magnet configured to collect the magnetic particles in the chambers; a movement mechanism configured to move the magnet in a direction different from a circumferential direction of a circle in which the rotation shaft is centered; a detector configured to detect the test substance; and a controller programmed to control the rotation mechanism and the movement mechanism so as to transfer the magnetic particles from one of the chambers to another one of the chambers.

First claim

What is claimed is:

1. A method comprising:
forming, on a magnetic particle, a complex of a test substance and a labeling substance in a cartridge, the cartridge comprising a plurality of chambers and a channel connecting the chambers;
attracting the magnetic particle by a magnet;
transferring the magnetic particle between the chambers, the transferring comprising:
moving the magnet attracting the magnetic particle to move the magnetic particle between one of the chambers and the channel; and
rotating the cartridge in a circumferential direction to move the magnetic particle through the channel while the magnetic particle is attracted by the magnet, wherein the magnet is moved in a direction different from the circumferential direction.

2. The method of claim 1 , wherein
the magnet attracting the magnetic particle is moved in a radial direction of a circle in which a rotation shaft supporting the cartridge is centered.

3. The method of claim 1 , wherein
the transferring comprises:
(i) moving the magnet attracting the magnetic particle in a first direction and thereby moving the magnetic particle from a first chamber to the channel;
(ii) rotating the cartridge and thereby moving the magnetic particle through the channel; and
(iii) moving the magnet attracting the magnetic particle in a second direction and thereby moving the magnetic particle from the channel to a second chamber.

4. The method of claim 1 , further comprising
detecting, with a detector, the test substance in the complex transferred from one of the chambers based on the labeling substance in the complex.

5. The method of claim 1 , wherein
the magnet is arranged movably in a vertical direction relative to the cartridge, and the attracting comprises approaching the magnet to the cartridge in the vertical direction.

6. The method of claim 5 , wherein
the magnetic particle is released from the magnet by moving away the magnet from the cartridge.

7. The method of claim 5 , wherein
the magnet is arranged movably in a vertical direction and a horizontal direction relative to the cartridge,
the attracting comprises approaching the magnet to the cartridge in the vertical direction, and
the transferring comprises moving the magnet in the horizontal direction.

8. The method of claim 1 , wherein
a plurality of complexes are formed on a plurality of magnetic particles, and
the method further comprising
dispersing the magnetic particles in one of the chambers by rotating the cartridge.

9. The method of claim 1 , wherein
the labeling substance is reacted with a reagent in one of the chambers to emit a luminescence.

10. The method of claim 1 , wherein
the complex is washed by a washing liquid in one of the chambers.

Assignees

• Sysmex Corp

Inventors

• Horii Kazuyoshi
• Nose Tomoyuki
• Fujiwara Takao
• Tomoda Sayuri
• Miida Yusuke
• Maekawa Yasunori
• Sasaki Noriaki

Classifications

• G01N35/00069

  whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• G01N35/0098

  involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C) · CPC title

• G01N33/582

  with fluorescent label · CPC title

• G01N21/76

  Chemiluminescence; Bioluminescence · CPC title