Methods relating to intestinal organ-on-a-chip
US-11976304-B2 · May 7, 2024 · US
Methods relating to intestinal organ-on-a-chip
US12247227B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12247227-B2 |
| Application number | US-202318242731-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 6, 2023 |
| Priority date | Sep 13, 2016 |
| Publication date | Mar 11, 2025 |
| Grant date | Mar 11, 2025 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
First claim
Opening claim text (preview).
What is claimed herein is: 1. A method of providing an in vitro intestinal model system, the method comprising: a) providing i) intestinal endothelial cells, ii) an intestinal enteroid or colonoid comprising primary intestinal epithelial cells, iii) a microfluidic culture device, the device comprising a porous membrane having a first surface and a second surface, said membrane in fluidic communication with a microchannel, said microchannel in fluidic communication with a source of fluid; b) disrupting said intestinal enteroid or colonoid comprising primary intestinal epithelial cells into enteroid or colonoid fragments; c) seeding said first surface of said porous membrane with said enteroid or colonoid fragments so as to create seeded primary intestinal epithelial cells; d) seeding said second surface of said porous membrane with said intestinal endothelial cells; e) expanding said seeded primary intestinal epithelial cells in a first media comprising Wnt-3A so as to create a monolayer of the intestinal epithelial cells; f) replacing said first media comprising Wnt-3A and culturing said intestinal epithelial cells with a second media lacking Wnt-3A; and g) culturing said intestinal endothelial cells in a third media comprising Wnt-3A. 2. The method of claim 1 , wherein said first media further comprises Noggin. 3. The method of claim 1 , wherein said first media further comprises a ROCK inhibitor. 4. The method of claim 1 , wherein said first media further comprises R-spondin.
Assignees
Inventors
Classifications
of vertebrates · CPC title
Adult fibroblasts · CPC title
Wnt; Frizzeled · CPC title
Gastrin; Cholecystokinins [CCK] · CPC title
Epidermal growth factor [EGF] · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US12247227B2 cover?
- Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
- Who is the assignee on this patent?
- Harvard College
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0697. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 11 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).