Cell culture chamber with improved cell-contacting surfaces

Patent metadata

Field	Value
Publication number	US-12247188-B2
Application number	US-202017077447-A
Country	US
Kind code	B2
Filing date	Oct 22, 2020
Priority date	Oct 24, 2019
Publication date	Mar 11, 2025
Grant date	Mar 11, 2025

Abstract

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

First claim

What is claimed is:

1. A cassette for use in an automated cell engineering system as a removable and replaceable element, comprising:
a. a high temperature chamber configured for carrying out activation, transduction and/or expansion of a cell culture at a temperature of between 35° C. to 40° C., the high temperature chamber including a cell culture chamber;
b. a low temperature chamber configured for storage of a reagent at a temperature of between 4° C. to 8° C.;
c. a thermal barrier adjacent the high temperature chamber and the low temperature chamber and separating the high temperature chamber from the low temperature chamber within the cassette; and
d. one or more fluidics pathways connected to the cell culture chamber, wherein the fluidics pathways provide recirculation, removal of waste and homogenous gas exchange and distribution of nutrients to the cell culture chamber without disturbing cells within the cell culture chamber;
wherein the cell culture chamber is a flat and non-flexible chamber, having a low chamber height and a cell-contacting surface, and the cell culture chamber is maintained in a substantially planar orientation in the cassette, and
wherein at least a portion of the cell-contacting surface comprises a non-porous, gas-permeable material, and the cell-contacting surface further includes multiple structures cut or made into the cell-contacting surface to add surface area to increase cell adhesion, wherein the multiple structures are sandwiched between sheets of the non-porous, gas-permeable material.

2. The cassette of claim 1 , wherein the cell culture chamber further comprises at least one of:
i. a distal port configured to allow for the removal of air bubbles from the cell culture chamber and/or as a recirculation port;
ii. a medial port configured to function as a recirculation inlet port; and
iii. a proximal port configured to function as a drain port for cell removal.

3. The cassette of claim 1 , wherein the cell-contacting surface comprises a plurality of separate sections, each section comprising the non-porous, gas-permeable material.

4. The cassette of claim 1 , wherein at least about 50% of the cell-contacting surface comprises the non-porous, gas-permeable material.

5. The cassette of claim 1 , wherein the entire cell-contacting surface comprises the non-porous, gas-permeable material.

6. The cassette of claim 1 , wherein the non-porous, gas-permeable material comprises silicone, flouroethylenepolypropylene (FEP), or ethyl vinyl olefin (EVO).

7. The cassette of claim 1 , having a chamber height of about 0.5 cm to about 4 cm.

8. The cassette of claim 1 , having a volume of about 50 ml to about 200 ml.

9. The cassette of claim 1 , wherein the cassette does not include a centrifugation chamber.

10. The cassette of claim 1 , wherein the cell-contacting surface further comprises a surface coating on the cell-contacting surface selected from the group consisting of:
i. a surface coating that activates a cell;
ii. a surface coating that modulates a biological pathway in a cell;
iii. a surface coating that enhances growth of a cell;
iv. a surface coating that improves adhesion of a cell;
V. a surface coating that inhibits a cell;
vi. a surface coating that responds to media conditions; and
vii. a surface coating that has controlled solubility.

11. The cassette of claim 10 , wherein the surface coating comprises an adhesion molecule.

12. The cassette of claim 11 , wherein the adhesion molecule is fibronectin or a modified fibronectin.

13. The cassette of claim 1 , wherein the cassette is pre-filled with culture media, activation reagent, and optionally a vector.

14. The cassette of claim 1 , further comprising one or more of a pH sensor, a glucose sensor, an oxygen sensor, a lactate sensor, a cell counting module, a carbon dioxide sensor, and/or an optical density sensor.

15. The cassette of claim 1 , further comprising one or more sampling ports and/or injection ports.

16. The cassette of claim 1 , further comprising an access port for connecting the cassette to an external device.

17. The cassette of claim 16 , wherein the external device includes an electroporation unit or an additional media source.

18. The cassette of claim 1 , wherein one or more of the fluidic pathways comprise a silicone-based tubing component that allows oxygenation through the tubing component.

19. The cassette of claim 1 , wherein a portion of the cell-contacting surface further comprises a surface treatment.

20. The cassette of claim 1 , wherein the low temperature chamber further includes a bag or other holder for the reagent.

Assignees

• Octane Biotech Inc
• Lonza Walkersville Inc

Inventors

• Trainor Nuala
• Abraham Eytan
• Smith Timothy
• Hewitt Matthew
• Shi Yaling
• Purpura Kelly
• Mcrobie Chase

Classifications

• C12M29/24

  Recirculation of gas · CPC title

• C12M41/48 Primary

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M41/32

  of substances in solution · CPC title

• C12M41/34

  of gas · CPC title

• C12M41/12

  of temperature (controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00) · CPC title