Pesticidal toxin proteins active against Lepidopteran insects

Patent metadata

Field	Value
Publication number	US-12240874-B2
Application number	US-202318319022-A
Country	US
Kind code	B2
Filing date	May 17, 2023
Priority date	Jan 12, 2017
Publication date	Mar 4, 2025
Grant date	Mar 4, 2025

Abstract

Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

First claim

What is claimed is:

1. A polynucleotide construct comprising a nucleotide sequence encoding:
an insecticidal protein having at least 95% identity to an amino acid sequence comprising amino acids 1 through 607 of SEQ ID NO:4, wherein said nucleotide sequence is operably linked to a heterologous promoter sequence.

2. A composition comprising the polynucleotide construct of claim 1 and the insecticidal protein encoded by the nucleotide sequence, wherein the insecticidal protein is toxic to black cutworm Lepidopteran species.

3. The polynucleotide construct according to claim 1 , wherein said insecticidal protein exhibits activity against Lepidopteran species selected from the group consisting of Spodoptera frugiperda, Spodoptera exigua, Spodoptera litura, Mamestra configurata, Striacosta albicosta, Trichoplusia ni, Pseudoplusia includens, Anticarsia gemmatalis, Hypena scabra, Heliothis virescens, Agrotis subterranea, Pseudaletia unipuncta, Agrotis ipsilon, Agrotis orthogonia, Ostrinia nubilalis, Amyelois transitella, Crambus caliginosellus, Herpetogramma licarsisalis, Homoeosoma electellum, Elasmopalpus lignosellus, Cydia pomonella, Endopiza viteana, Grapholita molesta, Suleima helianthana, Plutella xylostella, Pectinophora gossypiella, Lymantria dispar, Alabama argillacea, Archips argyrospila, Archips rosana, Chilo suppressalis, Cnaphalocrocis medinalis, Crambus teterrellus, Diatraea grandiosella, Diatraea saccharalis, Earias insulana, Egrias vittella, Helicoverpa armigera, Helicoverpa zea, Lobesia botrana, Pectinophora gossypiella, Phyllocnistis citrella, Pieris brassicae, Pieris rapae, Plutella xylostella , and Tuta absoluta.

4. The protein according to claim 2 further comprising bioactivity against Lepidopteran species selected from the group consisting of Spodoptera frugiperda, Spodoptera exigua, Spodoptera litura, Mamestra configurata, Striacosta albicosta, Trichoplusia ni, Pseudoplusia includens, Anticarsia gemmatalis, Hypena scabra, Heliothis virescens, Agrotis subterranea, Pseudaletia unipuncta, Agrotis ipsilon, Agrotis orthogonia, Ostrinia nubilalis, Amyelois transitella, Crambus caliginosellus, Herpetogramma licarsisalis, Homoeosoma electellum, Elasmopalpus lignosellus, Cydia pomonella, Endopiza viteana, Grapholita molesta, Suleima helianthana, Plutella xylostella, Pectinophora gossypiella, Lymantria dispar, Alabama argillacea, Archips argyrospila, Archips rosana, Chilo suppressalis, Cnaphalocrocis medinalis, Crambus teterrellus, Diatraea grandiosella, Diatraea saccharalis, Earias insulana, Egrias vittella, Helicoverpa armigera, Helicoverpa zea, Lobesia botrana, Pectinophora gossypiella, Phyllocnistis citrella, Pieris brassicae, Pieris rapae, Plutella xylostella , and Tuta absoluta.

5. A vector comprising the polynucleotide construct of claim 1 .

6. A host cell comprising the polynucleotide construct of claim 1 , wherein the host cell is selected from the group consisting of a bacterial cell, a yeast cell, and a plant cell.

7. The host cell of claim 6 , wherein said plant cell is selected from the group consisting of an alfalfa, banana, barley, bean, broccoli, cabbage, brassica, carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, citrus, coconut, coffee, corn, clover, cotton, a cucurbit, cucumber, Douglas fir, eggplant, eucalyptus, flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millets, melons, nut, oat, olive, onion, ornamental, palm, pasture grass, pea, peanut, pepper, pigeon pea, pine, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rootstocks, rye, safflower, shrub, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet corn, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat plant cell.

8. A plant comprising the polynucleotide construct of claim 1 .

9. A seed produced from the plant of claim 8 , wherein said seed comprises a detectable amount of said polynucleotide construct.

10. The plant of claim 8 , wherein seed, pollen, progeny, plant cells, plant tissue and commodity products produced from said plant comprise a detectable amount of said polynucleotide construct.

11. A biological sample comprising a detectable amount of the polynucleotide construct of claim 1 .

12. The composition of claim 2 further comprising:
(a) an agent different from said insecticidal protein and also toxic to the same Lepidopteran species, wherein said agent is selected from the group consisting of a polypeptide having an amino acid sequence different from said protein, an RNA molecule, and a chemical compound; or
(b) an agent selected from the group of toxin proteins consisting of: Cry1A, Cry1Ab, Cry1Ac, Cry1A.105, Cry1Ae, Cry1B, Cry1C, Cry1D, Cry1E, Cry1F, Cry1G, Cry1H, Cry1I, Cry1J, Cry1K, Cry1L, Cry2A, Cry2Ab, Cry2Ae, Cry4B, Cry6, Cry7, Cry8, Cry9, Cry15, Cry43A, Cry43B, ET66, TIC400, TIC800, TIC807, TIC834, TIC853, TIC1415, VIP3A, VIP3Ab, AXMI insecticidal proteins, DIG insecticidal proteins, eHIPs, and VIP proteins.

13. The composition of claim 12 , further comprising an additional pesticidal agent, wherein said additional agent is selected from the group of toxin proteins consisting of a Cry1C, a Cry3A, a Cry3B, a Cry34, a Cry35, Cry51Aa1, ET29, ET34, ET70, TIC407, TIC417, TIC431, TIC901, TIC1201, TIC3131, 5307, DIG-10, Axmi184, Axmi205 and AxmiR1.

14. A method of producing seed comprising the polynucleotide construct of claim 1 , said method comprising:
(b) planting seed comprising said polynucleotide construct;
(c) growing plants from said seed; and
(d) harvesting a crop of seed from said plants, wherein said crop of seed comprises said polynucleotide construct.

15. The polynucleotide construct of claim 1 , wherein said nucleotide sequence encodes an insecticidal polypeptide exhibiting at least 96% identity to an amino acid sequence comprising amino acids 1 through 607 of SEQ ID NO:4.

16. The polynucleotide construct of claim 1 , wherein said nucleotide sequence encodes an insecticidal polypeptide exhibiting at least 97% identity to an amino acid sequence comprising amino acids 1 through 607 of SEQ ID NO:4.

17. The polynucleotide construct of claim 1 , wherein said nucleotide sequence encodes an insecticidal polypeptide exhibiting at least 99% identity to an amino acid sequence comprising amino acids 1 through 607 of SEQ ID NO:4.

18. The polynucleotide construct of claim 1 , wherein said nucleotide sequence encodes an insecticidal polypeptide having the amino acid sequence from position 7 through 607 as set forth in SEQ ID NO:4.

19. The polynucleotide construct of claim 1 , wherein said nucleotide sequence encodes an insecticidal polypeptide having the amino acid sequence from position 1 through 607 as set forth in SEQ ID NO:4.

Assignees

• Monsanto Technology Llc

Inventors

• Baum James A
• Bowen David J
• Chay Catherine A
• Chi David J
• Clinton William P
• Dart Crystal L
• English Leigh
• Flasinski Stanislaw
• Guzov Victor M
• Jarrell Kevin A
• Kesanapalli Uma R
• Malvar Thomas M
• Mccarroll Robert M
• Milligan Jason S
• Morgenstern Jay P
• Rucker Deborah G
• Salvador Sara A
• Smith Temple F
• Soto Carlos E
• Stultz Collin M
• Turczyk Brian M
• Vaughn Ty T
• Von Rechenberg Moritz W F

Classifications

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• A01P7/04

  Insecticides · CPC title