Compositions and methods for controlling plant pests and improving plant health

Patent metadata

Field	Value
Publication number	US-12239137-B2
Application number	US-201917288786-A
Country	US
Kind code	B2
Filing date	Oct 29, 2019
Priority date	Oct 30, 2018
Publication date	Mar 4, 2025
Grant date	Mar 4, 2025

Abstract

Compositions and methods for controlling plant pests and/or improving at least one agronomic trait of interest in a plant are provided. Such compositions and methods comprise a bacterial strain that can be used as an inoculant for plants. Therefore, methods for growing a plant susceptible to a plant pest and/or plant disease caused by a plant pest and methods for controlling plant pests and/or plant disease on a plant susceptible to the plant pest and/or plant disease are also provided.

First claim

What is claimed is:

1. A method for controlling a plant pest population comprising contacting said population with a composition comprising an effective amount of a biocontrol agent, wherein the biocontrol agent comprises a Pseudomonas chlororaphis bacterial strain AIP059286 (Northern Region Research Laboratory (NRRL) No. B-67653), wherein said composition controls said plant pest.

2. The method of claim 1 , wherein the bacterial strain is present in the composition at about 10 5 colony forming units per gram (CFU/gram) to about 10 12 CFU/gram or in about 10 5 CFU/ml to about 10 12 CFU/ml.

3. The method of claim 2 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.

4. The method of claim 1 , wherein the composition further comprises a pesticide, a fungicide, an insecticide, or a herbicide.

5. The method of claim 4 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.

6. A method for growing a plant susceptible to a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:
(a) an effective amount of a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein said effective amount controls the plant pest.

7. The method of claim 6 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.

8. A method of controlling a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:
(a) an effective amount of a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein said effective amount controls the plant pest.

9. The method of claim 8 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.

10. The method of claim 8 , wherein the biocontrol agent is applied to the plant or plant part after harvest.

11. A method of treating or preventing a plant disease comprising applying to a plant or plant part having a plant pest or plant disease or at risk of developing a plant pest or plant disease:
(a) an effective amount of a biocontrol agent comprising a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein the effective amount controls the plant pest.

12. A stable spray-dried formulation comprising a biocontrol agent, wherein the biocontrol agent comprises
(a) a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653.

13. The stable spray-dried formulation of claim 12 , wherein said spray-dried formulation is dried to a water activity of 0.3 or less.

14. The stable spray-dried formulation of claim 12 , wherein the formulation is a wettable powder or a granule.

15. The stable spray-dried formulation of claim 12 , wherein the biocontrol agent is present at about 10 5 CFU/gram to about 10 12 CFU/gram or at about 10 5 CFU/ml to about 10 12 CFU/ml.

16. The stable spray-dried formulation of claim 12 , wherein the formulation further comprises a pesticide, a fungicide, an insecticide or an herbicide.

17. The stable spray-dried formulation of claim 16 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.

18. A coated seed comprising a seed and a coating on the seed, wherein the coating comprises a spray-dried formulation comprising a biocontrol agent, wherein the biocontrol agent comprises a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653).

19. The coated seed of claim 18 , wherein the coating further comprises a pesticide, a fungicide, an insecticide or an herbicide.

20. The coated seed of claim 19 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.

Assignees

• Agbiome Inc
• Ginkgo Bioworks Inc

Inventors

• Gachango Esther

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• A01P3/00

  Fungicides · CPC title

• A01P7/04

  Insecticides · CPC title

• A01N63/22

  Bacillus · CPC title

• A01N63/23

  B. thuringiensis · CPC title