Mixtures and compositions comprising Paenibacillus strains or fusaricidins and chemical pesticides

Patent metadata

Field	Value
Publication number	US-12239136-B2
Application number	US-201716076424-A
Country	US
Kind code	B2
Filing date	Feb 6, 2017
Priority date	Feb 9, 2016
Publication date	Mar 4, 2025
Grant date	Mar 4, 2025

Abstract

The present invention relates to novel mixtures comprising, as active components, at least one isolated bacterial strain, which is a member of the genus Paenibacillus , or a cell-free extract thereof or at least one metabolite thereof, and at least one chemical pesticide. The present invention also relates to compositions comprising at least one of such bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and at least one chemical pesticide. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to mixtures of fusaricidins which are pesticidal metabolites produced by the abovementioned strains, and chemical pesticides.

First claim

The invention claimed is:

1. A mixture, comprising as active components 1) at least one Paenibacillus strain selected from the group consisting of:
a) Paenibacillus polymyxa strain Lu16774 deposited with DSMZ under Accession No. DSM 26969;
b) Paenibacillus polymyxa strain Lu17007 deposited with DSMZ under Accession No. DSM 26970;
c) Paenibacillus epiphyticus strain Lu17015 deposited with DSMZ under Accession No. DSM 26971; and
d) a mutant of strain a) or b) which comprises a nucleic acid having a nucleotide sequence having
100% nucleotide sequence identity to the DNA sequences SEQ ID NO:4 or SEQ ID NO:9; or
100% nucleotide sequence identity to the DNA sequences SEQ ID NO:5 or SEQ ID NO:10; or
100% nucleotide sequence identity to the DNA sequences SEQ ID NO:6 or SEQ ID NO:11; or
100% nucleotide sequence identity to the DNA sequences SEQ ID NO:7 or SEQ ID NO:12; or
100%-nucleotide sequence identity to the DNA sequences SEQ ID NO: 8 or SEQ ID NO:13; and

e) a mutant of strain c) which comprises a nucleic acid having a nucleotide sequence having
100%-nucleotide sequence identity to the DNA sequence SEQ ID NO: 14; and
100%-nucleotide sequence identity to the DNA sequence SEQ ID NO: 15; and
100%-nucleotide sequence identity to the DNA sequence SEQ ID NO: 16; and
100%-nucleotide sequence identity to the DNA sequence SEQ ID NO: 17; and
100%-nucleotide sequence identity to the DNA sequence SEQ ID NO: 18;

wherein said at least one Paenibacillus strain produces a compound 1A and/or a compound 1B in a growth medium comprising at least one source of carbon and one source of nitrogen:

and

2) at least one pesticide selected from the group consisting of: Pyraclostrobin, Azoxystrobin, Dimoxystrobin, Orysastrobin, Picoxystrobin, Trifloxystrobin, Benzovindiflupyr, Fluopyram, Fluxapyroxad, Bixafen, Penflufen, Penthiopyrad, Sedaxane, Silthiofam, Difenoconazole, Metconazole, Prothioconazole, Triticonazole, Mefentrifluconazole, Epoxiconazole, Ipconazole, Tebuconazole, Triadimenol, Prochloraz, Metalaxyl, metalaxyl-M, Thiabendazole, Thiophanate methyl, Ethaboxam, Pyrimethanil, Fludioxonil, Dimethomorph, Thiram, and Picarbutrazox,
wherein the mixture comprises component 1) and component 2) in a synergistically effective amount.

2. The mixture according to claim 1 , wherein said at least one Paenibacillus strain has antifungal activity against at least two plant pathogens of the plant pathogen selected from the group consisting of Alternaria spp., Botrytis cinerea, Phytophthora infestans and Sclerotinia sclerotiorum.

3. A composition comprising the mixture as defined in claim 1 and an auxiliary.

4. The composition of claim 3 , comprising a further pesticide selected from the group consisting of:
pyraclostrobin, azoxystrobin, picoxystrobin, trifloxystrobin, dimoxystrobin, enestroburin, fenaminstrobin, fluoxastrobin, kresoxim-methyl, mandestrobin, metominostrobin, orysastrobin, pyrametostrobin, pyraoxystrobin,
fluxapyroxad, boscalid, benzovindiflupyr, penflufen, penthiopyrad, sedaxane, fluopyram, bixafen, isopyrazam,
carboxin, benodanil, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, thifluzamide,
silthiofam,
ipconazole, difenoconazole, prothioconazole, prochloraz, triticonazole, flutriafol, cyproconazole, diniconazole, diniconazole-M, fluquinconazole, flusilazole, hexaconazole, imazalil, imibenconazole, metconazole, myclobutanil, simeconazole, tebuconazole, triadimenol, uniconazole, thiabendazole,
oxathiapiprolin, valifenalate, metalaxyl, metalaxyl-M, ethaboxam, dimethomorph, zoxamide, flumorph, mandipropamid, pyrimorph, benthiavalicarb, iprovalicarb,
fludioxonil,
thiophanate-methyl, carbendazim,
thiram, ziram,
fipronil, ethiprole, vaniliprole, pyrafluprole, pyriprole, 5-amino-1-(2,6-dichloro-4-methyl-phenyl)-4-sulfinamoyl-1H-pyrazole-3-carbothioic acid amide,
chlorantraniliprole, flubendiamide,
cyantraniliprole,
tefluthrin, bifenthrin, cypermethrin, alpha-cypermethrin, cyfluthrin, beta-cyfluthrin, lambda-cyhalothrin, deltamethrin, esfenvalerate, etofenprox, fenvalerate, flucythrinate, permethrin,
clothianidin, imidacloprid, thiamethoxam, dinotefuran, acetamiprid, flupyradifurone, thiacloprid, triflumezopyrim, nitenpyram,
sulfoxaflor, acephate, chlorpyrifos, thiodicarb, abamectin, spinosad, and
tioxazafen,
wherein the further pesticide is different from the pesticide of component 2).

5. A plant propagation material having a coating comprising the composition as defined in claim 3 .

Assignees

• Basf Se

Inventors

• Siepe Isabella
• Liebmann Burghard
• Jabs Thorsten
• Schuster Annette

Classifications

• A01H3/00

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title

• A01N37/46 Primary

  N-acyl derivatives · CPC title