Sample injection with fluidic connection between fluid drive unit and sample accommodation volume

Patent metadata

Field	Value
Publication number	US-12235248-B2
Application number	US-202318369739-A
Country	US
Kind code	B2
Filing date	Sep 18, 2023
Priority date	May 30, 2016
Publication date	Feb 25, 2025
Grant date	Feb 25, 2025

Abstract

An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the separation unit and further fluid driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit. A control unit controls an outlet flow rate value according to which a combination of the mobile phase and the fluidic sample is driven through the separation unit.

First claim

What is claimed is:

1. An injector for injecting a fluidic sample into a flow path between a fluid drive and a separation unit of a sample separation apparatus, the injector comprising:
a sample accommodation volume configured to accommodate the fluidic sample prior to injecting;
a sample drive configured to intake the fluidic sample into the sample accommodation volume;
a fluidic valve switchable between multiple switching states to thereby selectively couple the sample accommodation volume with the flow path or decouple the sample accommodation volume from the flow path, wherein in an injection switching state of the fluidic valve, the fluid drive, the separation unit and the sample drive are fluidically coupled by the fluidic valve so that the fluidic sample driven by the sample drive and flowing from the sample accommodation volume to the separation unit and mobile phase driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit; and
a control unit configured to adjust an actual outlet flow rate value according to which a combination of the mobile phase and the fluidic sample is driven through the separation unit to obtain a target outlet flow rate value.

2. The injector according to claim 1 , wherein the control unit is configured to adjust the actual outlet flow rate value by adjusting at least one of a flow rate value in a fluidic path operated by the fluid drive or a flow rate value in a fluidic path operated by the sample drive.

3. The injector according to claim 1 , wherein the control unit is configured to adjust the actual outlet flow rate value by adjusting a volume over time displacement characteristic of a piston of at least one of the fluid drive or the sample drive.

4. The injector according to claim 1 , wherein the control unit is configured to adjust the actual outlet flow rate value by synchronizing respective operations of the fluid drive and the sample drive.

5. The injector according to claim 1 , wherein the control unit is configured to adjust an actual mixing ratio between the mobile phase driven by the fluid drive and the fluidic sample driven by the sample drive at the fluidic connection to obtain a target mixing ratio.

6. The injector according to claim 1 , wherein the fluidic connection is defined by a port of the fluidic valve.

7. The injector according to claim 1 , wherein the fluidic connection is located in an interior of the fluidic valve.

8. The injector according to claim 1 , wherein the fluidic valve is a rotatable fluidic valve having a rotor and a stator being rotatable relative to one another to bring different fluid ports of the stator in alignment with one or more fluid conduits of the rotor.

9. The injector according to claim 8 , wherein the fluidic connection is at least partially defined by one fluid port of the different fluid ports being fluidically coupled to one fluid conduit of the one or more fluid conduits at a central position of the one fluid conduit in the injection switching state, wherein the one fluid port is further fluidically connected to a capillary guiding towards the separation unit.

10. The injector according to claim 1 , wherein the control unit is configured to control a pressure at the fluidic connection during injection.

11. The injector according to claim 1 , wherein the control unit is configured to adjust an actual total pressure value according to which the combination of the mobile phase and the fluidic sample is driven through the separation unit to obtain a target total pressure value.

12. The injector according to claim 1 , wherein the fluidic valve comprises a stator and a rotor being rotatable relative to one another, and the fluidic valve comprises one of the following features:
the stator comprises a plurality of ports and at least one fluid conduit in permanent fluid communication with at least part of the plurality of ports, and the rotor comprises at least one fluid conduit;
the stator comprises a plurality of ports but no fluid conduits, and the rotor comprises at least one fluid conduit.

13. The injector according to claim 1 , wherein the sample drive is operable and the fluidic valve is switchable into a pressure adjustment switching state in which a predefined overpressure for injection is adjustable in the sample accommodation volume before switching the fluidic valve for injecting the fluidic sample towards the separation unit.

14. The injector according to claim 13 , wherein the sample drive is operable and the fluidic valve is switchable so that the predefined overpressure for injection triggers injection of the fluidic sample from the sample accommodation volume towards the separation unit by pressure equilibration, without piston motion and/or exclusively by pressure equilibration.

15. The injector according to claim 1 , wherein the fluidic valve is configured to be switchable into another injection switching state in which the fluidic sample is injected towards the separation unit driven by the fluid drive while the sample accommodation volume is located downstream of the fluid drive and upstream of the separation unit.

16. A sample separation apparatus for separating a fluidic sample, wherein the sample separation apparatus comprises:
the injector according to claim 1 ;
the fluid drive; and
the separation unit configured to separate the fluidic sample in the mobile phase.

17. The sample separation apparatus of claim 16 , further comprising at least one of the following features:
a detector configured to detect separated fractions of the fluidic sample;
a fractionating unit configured to collect separated fractions of the fluidic sample;
a degassing apparatus configured to degas the mobile phase;
the sample separation apparatus is configured as a chromatography sample separation apparatus.

18. A method of injecting a fluidic sample into a flow path between a fluid drive and a separation unit of a sample separation apparatus, the method comprising:
intaking the fluidic sample into a sample accommodation volume of an injector;
switching a fluidic valve of the injector into an injection switching state in which the fluidic valve fluidically couples the fluid drive, the sample drive and the separation unit so that the fluidic sample driven by the sample drive and flowing from the sample accommodation volume to the separation unit and mobile phase driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit to thereby inject the fluidic sample from the sample accommodation volume into the flow path in the injection switching state; and
adjusting an actual outlet flow rate value according to which a combination of the mobile phase and the fluidic sample is driven through the separation unit to obtain a target outlet flow rate value.

19. The method according to claim 18 , wherein the adjusting of the actual flow rate value comprises adjusting at least one of a flow rate value in a fluidic path operated by the fluid drive or a flow rate value in a fluidic path operated by the sample drive.

20. The method according to claim 18 , comprising adjusting an actual mixing ratio between the mobile phase driven by the fluid drive and the fluidic sample driven by the sample drive at the fluidic connection to obtain a target mixing ratio.

Assignees

• Agilent Technologies Inc

Inventors

• Thielsch Daniel
• Ortmann Thomas
• Wouters Sam

Classifications

• G01N2030/027

  Liquid chromatography · CPC title

• G01N2030/201

  multiport valves, i.e. having more than two ports · CPC title

• G01N2030/328

  valves, e.g. check valves of pumps · CPC title

• G01N2030/202

  rotary valves · CPC title

• G01N30/32

  of pressure or speed (G01N30/36 takes precedence) · CPC title