Compositions and methods for the assessment of soil quality

Patent metadata

Field	Value
Publication number	US-12235218-B2
Application number	US-201917271768-A
Country	US
Kind code	B2
Filing date	Sep 3, 2019
Priority date	Aug 31, 2018
Publication date	Feb 25, 2025
Grant date	Feb 25, 2025

Abstract

Disclosed are methods of measuring the quantity of active organic matter in a soil sample, as well as kits for performing such measurements.

First claim

What is claimed is:

1. A method for assessing a quantity of active organic matter in a soil sample, the method comprising:
(a) contacting a soil sample with an aqueous solution comprising a permanganate salt, an inorganic flocculant, and a pH buffering agent, wherein the aqueous solution is buffered at a pH of from 5 to less than 7; and
(b) evaluating the aqueous solution to assess the quantity of active organic matter in the soil sample.

2. The method of claim 1 , wherein the permanganate salt comprises potassium permanganate.

3. The method of claim 1 , wherein the permanganate salt is present in the aqueous solution at a concentration of from 0.01 M to 0.05 M.

4. The method of claim 1 , wherein the aqueous solution has is buffered at a pH of from 5.5 to 6.5.

5. The method of claim 1 , wherein the pH buffering agent also acts as an inorganic complexing agent.

6. The method of claim 1 , wherein the pH buffering agent is present in the aqueous solution at a concentration of from 0.0005 M to 0.01 M.

7. The method of claim 1 , wherein the inorganic flocculant is present in the aqueous solution at a concentration of from 0.01 M to 0.2 M.

8. The method of claim 1 , wherein the inorganic flocculant exhibits a positive enthalpy of solvation in water.

9. The method of claim 1 , wherein step (b) comprises:
visually comparing a color of the aqueous solution with a color chart to determine the concentration of active organic matter in the soil sample, wherein the color chart comprising a plurality of colors and a concentration of active organic matter corresponding to each of the plurality of colors;
measuring an absorbance value of the aqueous solution using a spectrophotometer, and determining the concentration of active organic matter in the soil sample from the absorbance value using a standard curve that correlates absorbance values at a wavelength with concentrations of active organic matter in soil samples;
measuring an absorbance value of the aqueous solution using a colorimeter, and determining the concentration of active organic matter in the soil sample from the absorbance value using a lookup table that lists concentrations of active organic matter in soil samples for a plurality of absorbance values;
imaging the aqueous solution, obtaining one or more RGB values from an image of the aqueous solution, and determining the concentration of active organic matter in the soil sample from the one or more RGB values using a lookup table that lists concentrations of active organic matter in soil samples for a plurality of RGB values; or
imaging the aqueous solution, obtaining one or more RGB values from an image of the aqueous solution, and determining the concentration of active organic matter in the soil sample from the absorbance value using a standard curve that correlates one or more RGB values with concentrations of active organic matter in soil samples.

10. The method of claim 1 , further comprising deriving one or more additional soil quality parameters from the quantity of active organic matter in the soil sample,
wherein the one or more additional soil quality parameters are selected from the group consisting of microbial biomass, basal respiration, total nitrogen content, active nitrogen content, particulate organic carbon, aggregate stability, soil quality, or any combination thereof.

11. The method of claim 1 , further comprising deriving a projected crop yield or projected relative crop yield from the quantity of active organic matter in the soil sample.

12. The method of claim 1 , wherein the soil sample comprises an oxisol, a mollisol, an alfisol, an ultisol, an entisol, an inceptisol, or any combination thereof.

13. The method of claim 1 , wherein the pH buffering agent comprises a phosphate salt.

14. The method of claim 13 , wherein the phosphate salt comprises dipotassium phosphate.

15. The method of claim 1 , wherein the inorganic flocculant comprises a chloride salt.

16. The method of claim 15 , wherein the chloride salt is selected from the group consisting of calcium chloride, aluminum chloride, and combinations thereof.

17. The method of claim 1 , wherein step (a) comprises:
(i) combining the soil sample and the aqueous solution in a vessel;
(ii) agitating the soil sample and the aqueous solution; and
(iii) incubating the soil sample and the aqueous solution.

18. The method of claim 17 , wherein step (iii) comprises incubating the soil sample and the aqueous solution for a period of time sufficient to allow the soil sample to settle out of the aqueous solution and a color of the aqueous solution to equilibrate.

19. A method for cultivating a crop in an agricultural field, the method comprising:
planting the crop in the agricultural field;
allowing the crop to reach at least a seedling stage;
collecting a soil sample from the agricultural field;
determining a quantity of active organic matter in the soil sample using the method of claim 1 ;
deriving a quantity of active nitrogen in the soil sample from the quantity of active organic matter measured in the soil sample; and
applying a fertilizer to the agricultural field when the quantity of active nitrogen in the soil sample is less than 1600 kg/ha.

Assignees

• Ohio State Innovation Foundation

Inventors

• Islam Khandakar

Classifications

• G01N33/245

  for agricultural purposes · CPC title

• G01N33/24

  Earth materials (G01N33/42 takes precedence) · CPC title

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title

• A01C21/007

  Determining fertilization requirements · CPC title

• G01N33/243

  for determining biological parameters concerning composting, biodegradability or bioavailability · CPC title