Resin film formed by scaffold material for cell culture and container for cell culture

Patent metadata

Field	Value
Publication number	US-12234485-B2
Application number	US-202017608587-A
Country	US
Kind code	B2
Filing date	May 27, 2020
Priority date	May 28, 2019
Publication date	Feb 25, 2025
Grant date	Feb 25, 2025

Abstract

Provided is a resin film having excellent cell extensibility in cell culture. The resin film formed of a scaffold material for cell culture containing a synthetic resin, the resin film having a compressive modulus of 5.5 GPa or more at a frequency of 1 Hz as measured using a nanoindenter device in ion-exchanged water in accordance with ISO14577-1 after being immersed in the ion-exchanged water at 37° C. for 24 hours.

First claim

The invention claimed is:

1. A method of culturing a cell, comprising a step of culturing a cell on a resin film formed of a scaffold material containing a synthetic resin,
the resin film having a compressive modulus of 5.5 GPa or more and 15 GPa or less at a frequency of 1 Hz as measured using a nanoindenter device in ion-exchanged water in accordance with ISO14577-1 after being immersed in the ion-exchanged water at 37° C. for 24 hours, and
the resin film having a contact angle in water of less than 145° and 100° or more in ion-exchanged water at 16° C.

2. The method according to claim 1 , wherein the resin film has a contact angle in water of less than 140° and 100° or more in ion-exchanged water at 16° C.

3. The method resin film according to claim 1 , wherein the synthetic resin has a number average molecular weight of 4.0×10 4 or more and 150×10 4 or less.

4. The method according to claim 3 , wherein the synthetic resin contains a polyvinyl alcohol derivative or a poly (meth) acrylic ester derivative.

5. The method according to claim 4 , wherein the polyvinyl alcohol derivative is a polyvinyl acetal resin X,
the polyvinyl acetal resin X is a synthetic resin having a polyvinyl acetal skeleton.

6. The method according to claim 5 , wherein the polyvinyl acetal skeleton is a polyvinyl butyral skeleton.

7. The method according to claim 5 , wherein the polyvinyl acetal resin X has a structural unit having an imine structure, a structural unit having an imide structure, a structural unit having an amine structure, a structural unit having an amide structure or any combinations thereof.

8. The method according to claim 5 , wherein the synthetic resin has a polyvinyl acetal skeleton and a poly (meth) acrylic ester skeleton.

9. The method according to claim 1 , wherein the cell is a stem cell.

10. The method according to claim 9 , wherein the stem cell is an iPS cell.

11. The method according to claim 1 , wherein the resin film is arranged on a surface of a container main body.

Assignees

• Sekisui Chemical Co Ltd

Inventors

• Arai Yuuhei
• Ishii Ryoma
• Iguchi Hiroki
• Haneda Satoshi

Classifications

• C12N2533/30

  Synthetic polymers · CPC title

• C12N2513/00

  3D culture · CPC title

• C12M3/00

  Tissue, human, animal or plant cell, or virus culture apparatus · CPC title

• C12M1/22

  Petri dishes · CPC title

• C12M1/00

  Apparatus for enzymology or microbiology · CPC title