Crosslinker comprising genipin for use in preparation of sensing film or diffusion control film of electrochemical sensor

Patent metadata

Field	Value
Publication number	US-12228542-B2
Application number	US-201916972540-A
Country	US
Kind code	B2
Filing date	May 9, 2019
Priority date	Jun 8, 2018
Publication date	Feb 18, 2025
Grant date	Feb 18, 2025

Abstract

The present disclosure relates to a composition for a crosslinking agent for the preparation of a sensing layer or a diffusion control layer of an electrochemical biosensor comprising genipin or a derivative thereof, in which due to the properties of genipin that can be extracted and used from plants, not only it has high biocompatibility compared to conventional crosslinking agents with high toxicity, but also it can easily confirm the progress of the reaction by measuring UV or measuring the amount of amine groups present when reacting with a compound having an amine group, for example, an electron transport medium, and additionally, it has the advantage of suppressing a rapid decrease in sensor life due to high concentration of glucose while maintaining an appropriate sensitivity to glucose.

First claim

The invention claimed is:

1. A sensing layer of an electrochemical sensor, comprising:
(i) a crosslinking agent for preparation of an electrochemical sensor comprising genipin represented by the following Chemical Formula 1, and
(ii) a polymer having N atoms selected from the group consisting of polyvinyl pyrrolidone (PVP) and polyvinyl imidazole:

2. The sensing layer of an electrochemical sensor according to claim 1 , further comprising an electron transport medium or an oxidoreductase.

3. The sensing layer of an electrochemical sensor according to claim 2 , wherein the oxidoreductase is one or more selected from the group consisting of dehydrogenase, oxidase, and esterase.

4. The sensing layer of an electrochemical sensor according to claim 2 , comprising, together with the oxidoreductase, one or more cofactors selected from the group consisting of flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), and pyrroloquinoline quinone (PQQ).

5. A method for preparing an electrochemical biosensor, comprising preparing the sensing layer according to claim 1 using genipin as a crosslinking agent.

6. The method according to claim 5 , wherein use of genipin of the following Chemical Formula 1 as a crosslinking agent for the preparation of an electrochemical sensor:

7. An electrochemical sensor, comprising the sensing layer according to claim 1 .

8. The electrochemical biosensor according to claim 7 , further comprising two or more electrode, an insulator and a substrate.

9. The electrochemical sensor according to claim 7 , wherein the sensor is for measuring blood glucose levels.

10. The electrochemical sensor according to claim 9 , wherein the sensor is a continuous blood glucose monitoring sensor.

11. A diffusion control layer of an electrochemical sensor comprising:
(i) a crosslinking agent for the preparation of an electrochemical sensor, comprising genipin represented by the following Chemical Formula 1, and
(ii) a polymer having N atoms selected from the group consisting of polyvinyl pyrrolidone (PVP) and polyvinyl imidazole:

12. An electrochemical sensor, comprising the diffusion control layer according to claim 11 .

13. The electrochemical biosensor according to claim 12 , further comprising two or more electrode, an insulator and a substrate.

14. The electrochemical sensor according to claim 12 , wherein the sensor is for measuring blood glucose levels.

15. The electrochemical sensor according to claim 14 , wherein the sensor is a continuous blood glucose monitoring sensor.

16. A method for preparing an electrochemical biosensor, comprising preparing a diffusion control layer according to claim 11 using genipin as a crosslinking agent.

17. The method according to claim 16 , wherein use of genipin of the following Chemical Formula 1 as a crosslinking agent for the preparation of an electrochemical sensor:

Assignees

• I Sens Inc

Inventors

• Jeong In Seok
• Yang Hyunhee
• Kang Young Jea

Classifications

• G01N27/3271

  Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001) · CPC title

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• C08K5/1545 Primary

  Six-membered rings · CPC title

• C07D311/94

  condensed with rings other than six-membered or with ring systems containing such rings · CPC title

• C12Q1/006 Primary

  for glucose · CPC title